CAREER: Dynamic and Intrinsic Profiling of Membrane Microdomain Composition, Function and Regulation

职业：膜微域组成、功能和调节的动态和内在分析

• 批准号: 1149452
• 负责人: Susan Daniel
• 金额: $ 40万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1149452&HistoricalAwards=false
• 关键词: CAREER; Dynamic; Intrinsic; Profiling; Membrane

CBET 1149452/ DanielThis NSF CAREER award by the Biotechnology, Biochemical and Biomass Engineering program supports a research and education program focused on discovering the function of species that comprise cell membrane microdomains and promoting the advancement of women in STEM disciplines through professional development. Membrane species are essential for regulating myriad cellular processes and vital to proper function of bioanalytical assays and sensors that employ these species as transducer elements. Yet our understanding of the links between protein function and the impact of the local membrane environment of that protein on its function is lacking. In this work, we will employ a novel microfluidic device comprised of patterned, two-phase co-existent membranes to assess biomolecule partitioning into different membrane phases; identify the stimuli that trigger changes in partitioning; and quantify how partitioning impacts, and possibly regulates, protein function. Knowing how protein function depends on the local membrane composition is vital to understanding membrane biology and appreciating how the membrane environment impacts drug-protein interactions is necessary for assessing therapeutic efficacy in mitigating diseases such as Diabetes, Alzheimer's disease, and infertility, where changes in cell membrane composition are implicated in the disease manifestation. Thus the broader impacts of this research are filling a technological gap in membrane proteomic research that will be used to study how partitioning depends on membrane properties and external stimuli (chemical, biological, environmental) to ultimately monitor direct interactions between species in heterogeneous membranes and determine protein activity changes after interaction. Coincident to the research plan, educational efforts on coaching graduate and post doctoral women to become successful leaders and educators will be implemented through a new multi-layered professional development program. The education plan will build leadership skills and educational competency through peer mentoring, teaching leadership skills through outreach programs, and career networking workshops. The broader impacts of this education plan are filling the gap between undergraduate advancement programs and professional development programs for young female professionals in the STEM disciplines; creating a model for female graduate student education that combines technical education in engineering with leadership and management skills to yield well-rounded, technically competent graduates; and ultimately increasing the number of women choosing advanced technical careers after graduate school.

CBET 1149452/ DanielThis NSF CAREER award by the Biotechnology，Biochemical and Biomass Engineering program supports a research and education program focused on discovering the function of species that comprise cell membrane microdomains and promoting the advancement of women in STEM disciplines through professional development.膜物种对于调节无数细胞过程是必不可少的，并且对于采用这些物种作为换能器元件的生物分析测定和传感器的正常功能至关重要。然而，我们对蛋白质功能与该蛋白质的局部膜环境对其功能的影响之间的联系缺乏了解。在这项工作中，我们将采用一种新型的微流体装置，由图案化的两相共存膜组成，以评估生物分子分配到不同的膜相;识别触发分配变化的刺激;并量化分配如何影响，并可能调节蛋白质功能。 了解蛋白质功能如何取决于局部膜组成对于理解膜生物学和了解膜环境如何影响药物-蛋白质相互作用对于评估减轻疾病如糖尿病、阿尔茨海默病和不育症的治疗功效是必要的，其中细胞膜组成的变化与疾病表现有关。因此，这项研究的更广泛影响正在填补膜蛋白质组学研究的技术空白，该研究将用于研究分配如何取决于膜特性和外部刺激（化学，生物，环境），以最终监测异质膜中物种之间的直接相互作用，并确定相互作用后的蛋白质活性变化。与研究计划相一致的是，将通过一个新的多层次专业发展方案，开展教育工作，指导研究生和博士后妇女成为成功的领导者和教育工作者。该教育计划将通过同伴指导、通过外联方案教授领导技能和职业网络讲习班来培养领导技能和教育能力。这一教育计划的更广泛影响是，填补了科学、技术、工程和数学学科中年轻女性专业人员的本科进修方案与专业发展方案之间的差距;创建了一个女性研究生教育模式，将工程技术教育与领导和管理技能相结合，以培养全面发展、技术能力强的毕业生;并最终增加了研究生毕业后选择高级技术职业的女性人数。