CAREER: Integrative Physiology of Anoxia Tolerance in Turtles

职业：海龟耐缺氧的综合生理学

• 批准号: 1253939
• 负责人: Daniel Warren
• 金额: $ 90.39万
• 依托单位: Saint Louis University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2022-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1253939&HistoricalAwards=false
• 关键词: CAREER; Integrative; Physiology; Anoxia; Tolerance

All animals are subject to environmental stress during their lives and oxygen availability may be a particular problem for animals in aquatic habitats, where solubility is low and competition for it is high. One animal, the Western painted turtle has evolved the capacity to survive complete oxygen deprivation, also called anoxia, for over 30 hours at 20°C and for more than three months at 3°C, which is longer than any other tetrapod. This ability allows the painted turtle to overwinter in oxygen-depleted, ice-covered ponds in the wild. To understand the underlying mechanisms, this research uses the recently sequenced painted turtle genome to determine the gene expression changes that occur during anoxia and to relate them to the physiological changes that are already known to occur. It focuses on the turtle's heart, which, in other vertebrates, is extremely sensitive to oxygen deprivation, including humans. It also focuses on development, because hatchling turtles are less tolerant of anoxia than adults. This research also explores processes related to the handling of the massive lactic acid loads, which accumulate to levels higher than seen in any other animal. This includes studies of how lactic acid affects the turtle shell and heart and how that lactic acid metabolized by the body. There is also educational component involving the St. Louis Zoo, which will enhance one of its existing educational apprenticeship program representing mostly underserved and underrepresented minorities. It also supports an annual workshop to promote civic science, which will provide resources and opportunities for St. Louis area scientists to connect with high school and college students looking to gain scientific research experience. The broader impacts of this project will be to provide important insights into an economically, ecologically, and clinically relevant problem, namely why some animals are more tolerant of oxygen deprivation than others. It will also increase student participation in the sciences, generally, and comparative animal physiology, specifically.

所有动物在其生命中都会受到环境压力的影响，对于水生生境中的动物来说，氧气的可用性可能是一个特别的问题，因为水生生境中的溶解度低，对氧气的竞争很大。一种动物，西方彩龟已经进化出完全缺氧的生存能力，也称为缺氧，在20°C下超过30小时，在3°C下超过三个月，这比任何其他四足动物都要长。这种能力使彩龟能够在野外缺氧、冰封的池塘中过冬。为了了解潜在的机制，这项研究使用最近测序的彩绘龟基因组来确定缺氧期间发生的基因表达变化，并将其与已知发生的生理变化联系起来。它关注的是海龟的心脏，在其他脊椎动物中，这对缺氧非常敏感，包括人类。它也关注发展，因为幼龟比成年龟更不耐缺氧。这项研究还探索了与处理大量乳酸负荷相关的过程，这些乳酸负荷积累到比任何其他动物都高的水平。这包括研究乳酸如何影响龟壳和心脏，以及乳酸如何被身体代谢。圣路易斯动物园也有教育部分，这将加强其现有的教育学徒计划之一，代表大多数服务不足和代表性不足的少数民族。 它还支持每年举办一次促进公民科学的研讨会，这将为圣路易斯地区的科学家提供资源和机会，使他们能够与希望获得科学研究经验的高中生和大学生建立联系。该项目的更广泛的影响将是对经济，生态和临床相关问题提供重要的见解，即为什么有些动物比其他动物更能忍受缺氧。它还将增加学生对科学的参与，一般来说，特别是比较动物生理学。

项目成果

The metabolic consequences of repeated anoxic stress in the western painted turtle, Chrysemys picta bellii

西方锦龟反复缺氧应激的代谢后果

• DOI: 10.1016/j.cbpa.2016.07.012
• 发表时间: 2017
• 期刊: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• 作者: Warren, Daniel E.;Jackson, Donald C.
• 通讯作者: Jackson, Donald C.

Changes in the material properties of the shell during simulated aquatic hibernation in the anoxia-tolerant painted turtle

• DOI: 10.1242/jeb.176990
• 发表时间: 2018-09-01
• 期刊: JOURNAL OF EXPERIMENTAL BIOLOGY
• 影响因子: 2.8
• 作者: Odegard, Dean T.;Sonnenfelt, Michael A.;Warren, Daniel E.
• 通讯作者: Warren, Daniel E.