Collaborative Research: EAGER: The Role of Cell-Cell Forces in the Cadherin Switch Model
合作研究:EAGER:细胞-细胞力在钙粘蛋白开关模型中的作用
基本信息
- 批准号:1264988
- 负责人:
- 金额:$ 8.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-02-01 至 2014-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The research objective of this EArly-Concept Grant for Exploratory Research (EAGER) is to establish the link between the changing cadherin expression, or the cadherin switch model, and the evolving mechanical microenvironment that differentiating mesenchymal stem cells (MSCs) encounter during bone formation. MSCs experience both cell-matrix, or integrin-mediated, and cell-cell, or cadherin-mediated, forces during differentiation. However, the role of cell-cell forces through cadherins has been largely neglected despite that cadherin regulation is essential to normal bone formation. The "cadherin switch" specifies that as MSCs differentiate in to bone forming cells, one cadherin is up regulated and another is down regulated. The switch parallels the changing mechanical environment during bone formation: MSCs begin in soft marrow (~1kPa) and differentiate into bone matrix-producing cells in stiff, unmineralized matrix (10s of kPa). This work aims to relate the cadherin switch to the changing mechanical microenvironment for MSCs. The approach uses a combination of two tools: Electrohydrodynamic (E-Jet) and 3D, full-field traction force microscopy (3D TFM). E-Jet will pattern both proteins and stiffness on polyacrylamide substrates and enable manipulation cell-cell and cell-substrate forces. 3D TFM will measure the forces thus quantitatively establishing the relationship. The influence of cell-cell forces in differentiation and bone formation has largely been neglected. Results from this work could lead to new treatments for bone diseases and a more complete understanding of tissue development both for bone and for other tissues. Through the work the relatively new techniques, 3D TFM and E-Jet patterning of cell culture substrates, will be made more accessible to a broader scientific community. The educational component focuses on graduate student training and research experiences through interactions between institutions and across disciplines. The research will also be used to highlight the breadth of the field of Mechanical Engineering (ME) specifically for the Campus Middle School (CMS) for Girls, with the goal of making ME more accessible to these and other K-12 students who otherwise may not be interested in ME based on preconceptions of the field.
EARLY概念探索性研究资助(EAGER)的研究目标是建立钙粘蛋白表达变化或钙粘蛋白开关模型与分化间充质干细胞(MSC)在骨形成过程中遇到的不断变化的机械微环境之间的联系。 MSC在分化过程中经历细胞-基质或整合素介导的力和细胞-细胞或钙粘蛋白介导的力。 然而,尽管钙粘蛋白调节对正常骨形成至关重要,但通过钙粘蛋白的细胞-细胞力的作用在很大程度上被忽视了。 “钙粘蛋白开关”规定,当MSC分化成骨形成细胞时,一种钙粘蛋白上调,另一种钙粘蛋白下调。 这种转换与骨形成过程中不断变化的机械环境平行:MSC开始于软骨髓(~ 1 kPa)中,并在坚硬的未矿化基质(10 s kPa)中分化为骨基质生成细胞。 这项工作的目的是将钙粘蛋白开关与MSC不断变化的机械微环境联系起来。 该方法使用两种工具的组合:电流体动力学(E-Jet)和3D,全场牵引力显微镜(3D TFM)。 E-Jet将在聚丙烯酰胺基质上对蛋白质和硬度进行图案化,并能够操纵细胞-细胞和细胞-基质力。 3D TFM将测量力,从而定量地建立关系。细胞-细胞力在分化和骨形成中的影响在很大程度上被忽视了。 这项工作的结果可能会导致骨骼疾病的新治疗方法,以及对骨骼和其他组织的组织发育的更完整的理解。 通过这项工作,相对较新的技术,细胞培养基质的3D TFM和E-Jet图案化,将更容易为更广泛的科学界所接受。 教育部分侧重于研究生培训和研究经验,通过机构之间和跨学科的互动。 该研究还将用于突出机械工程(ME)领域的广度,特别是针对校园中学(CMS)的女孩,目的是使我更容易接近这些和其他K-12学生,否则他们可能对ME不感兴趣基于该领域的先入之见。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Amy Wagoner Johnson其他文献
An Indentation-Based Framework for Probing the Glycosaminoglycan-Mediated Interactions of Collagen Fibrils
用于探测糖胺聚糖介导的胶原纤维相互作用的基于压痕的框架
- DOI:
10.2139/ssrn.4279323 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
A. Ostadi Moghaddam;M. Arshee;Zixi Lin;M. Sivaguru;Heidi Phillips;Barbara McFarlin;Kimani Toussaint;Amy Wagoner Johnson - 通讯作者:
Amy Wagoner Johnson
Transcriptomic profiling of fetal membranes in a mouse model of preterm birth
- DOI:
10.1016/j.placenta.2017.07.123 - 发表时间:
2017-09-01 - 期刊:
- 影响因子:
- 作者:
Priyadarshini Pantham;Don Armstrong;Carmen Valero;Owen Haupt;Lori Underhill;Renato Iozzo;Amy Wagoner Johnson;Beatrice Lechner;Derek Wildman - 通讯作者:
Derek Wildman
Bone-Like Behavior of Brittle, Porous Hydroxyapatite Implants With Microporosity
- DOI:
10.1016/j.joms.2006.06.091 - 发表时间:
2006-09-01 - 期刊:
- 影响因子:3.5
- 作者:
Amy Wagoner Johnson - 通讯作者:
Amy Wagoner Johnson
Amy Wagoner Johnson的其他文献
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{{ truncateString('Amy Wagoner Johnson', 18)}}的其他基金
Collaborative Research: ECO-CBET: From Molecules to Sustainable Reef Platforms: Engineering Ecosystems for Coral Recruitment and Survival
合作研究:ECO-CBET:从分子到可持续珊瑚礁平台:珊瑚招募和生存的工程生态系统
- 批准号:
2133675 - 财政年份:2021
- 资助金额:
$ 8.6万 - 项目类别:
Continuing Grant
Convergence: RAISE: Engineering Coral Reef Recovery
融合:RAISE:工程珊瑚礁恢复
- 批准号:
1848671 - 财政年份:2018
- 资助金额:
$ 8.6万 - 项目类别:
Continuing Grant
Collaborative Research: Bone Adaptation-Driven Design of Scaffolds with Spatially-Varying Architecture for Enhanced Growth
协作研究:具有空间变化架构的骨骼适应驱动支架设计,以促进生长
- 批准号:
1727381 - 财政年份:2017
- 资助金额:
$ 8.6万 - 项目类别:
Standard Grant
Collaborative Research: Regulators of cellular microenvironment and multiscale osteointegration
合作研究:细胞微环境调节剂和多尺度骨整合
- 批准号:
1106165 - 财政年份:2011
- 资助金额:
$ 8.6万 - 项目类别:
Continuing Grant
Customized Load-Bearing Scaffolds Using Multiscale Porosity and Multi-Material Domains
使用多尺度孔隙度和多材料域的定制承重脚手架
- 批准号:
0900184 - 财政年份:2009
- 资助金额:
$ 8.6万 - 项目类别:
Standard Grant
A New Approach for Structure-Property Relations in Scaffold Design for Bone Tissue Engineering
骨组织工程支架设计中结构-性能关系的新方法
- 批准号:
0728246 - 财政年份:2007
- 资助金额:
$ 8.6万 - 项目类别:
Standard Grant
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