Generation of composites from borides with tuneable electrical conductivities using peptides optimized by genetic engineering; characterization of the bio-solid interactions by modelling and AFM

使用通过基因工程优化的肽从硼化物生成具有可调电导率的复合材料；

• 批准号: 210503983
• 负责人: Professorin Dr. Barbara Albert
• 金额: --
• 依托单位: Rektorat
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/210503983?language=en
• 关键词: Generation; composites; borides; tuneable; electrical

In collaboration with the ITB group, an interaction of inorganic materials with peptides shall be created in a chemobiological composite of phages and boride particles. Binding properties are to be optimized by genetic engineering which will employ a screening system based on biopanning specific peptides with preferences for different borides with different physical properties. Two model systems are used: 1) for a start a metallic, magnetic boride in form of wet-chemically synthesized nanoparticles 2) later on a boron-rich ceramic which is very high-temperature stable and inert.The key factors determining the preference of these peptides for a certain material shall be determined by molecular simulation. With the help of quantum chemical calculations, force fields for solid surfaces and solvents and peptides in contact with these surfaces are to be developed. Quantification will be performed experimentally by atomic force microscopy. The peptide-modified borides will be analysed for their magnetism and electrical conductivity, as well as thermal stability. This requires the modification and adjustment of established methods of physical properties measurements to composite samples. Later, the system will be modified for ceramic borides and specific peptides shall be developed for these as well. Bifunctional phages with differently optimized peptides might be used to combine different metallic and semiconducting borides or both metallic and ceramic inorganics into nano- and micro-structured composites with unforeseeable properties. We also envision small proteins consisting of different peptide sequences each optimized for a specific inorganic material. This way a well-defined spatial orientation of functional materials may be achieved. Basic knowledge gained from a joint experimental and theoretical study of bonding interactions between peptides and boride materials is crucial for further development of smart materials based on non-oxide solids. It can potentially pave the way for a wealth of applications such as thermoelectric devices, field effect transistors or biomedical systems. The project builds on the strong interaction between researchers from molecular biology and solid state chemists, as well as theoretically working groups and experts on atomic force microscopy.

与ITB小组合作，将在噬菌体和硼化物颗粒的化学生物学复合材料中创建无机材料与肽的相互作用。结合特性将通过基因工程进行优化，该系统将采用基于生物筛选的特定肽的筛选系统，对具有不同物理性质的不同硼化物具有偏好。使用了两种模型系统：1)首先是一种金属磁性硼化物，以湿化学合成的纳米颗粒的形式；2)然后是一种富含硼的陶瓷，这种陶瓷在高温下是非常稳定和惰性的。决定这些多肽对某种物质的偏好的关键因素应通过分子模拟来确定。在量子化学计算的帮助下，固体表面和与这些表面接触的溶剂和肽的力场将被开发出来。定量将通过原子力显微镜进行实验。将分析肽修饰的硼化物的磁性和导电性，以及热稳定性。这需要修改和调整现有的复合样品物理性质测量方法。随后，该系统将针对陶瓷硼化物进行改进，并将针对这些化合物开发特定的肽。具有不同优化肽的双功能噬菌体可用于将不同的金属和半导体硼化物或金属和陶瓷无机物结合成具有不可预见性能的纳米和微结构复合材料。我们还设想由不同肽序列组成的小蛋白质，每个肽序列针对特定的无机材料进行优化。通过这种方式，功能材料的空间取向可以得到明确的定义。从多肽和硼化物材料之间键合相互作用的联合实验和理论研究中获得的基础知识对于进一步开发基于非氧化物固体的智能材料至关重要。它可能为热电器件、场效应晶体管或生物医学系统等大量应用铺平道路。该项目建立在分子生物学和固态化学家的研究人员以及原子力显微镜的理论工作组和专家之间的强烈互动之上。