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Regulation of outer membrane biogenesis components LptD and Omp85 in cyanobacteria Anabaena sp. PCC 7120

蓝藻鱼腥藻外膜生物合成成分 LptD 和 Omp85 的调节。

基本信息

批准号：
210961441
负责人：
Professor Dr. Enrico Schleiff
金额：
--
依托单位：
Präsident der Goethe-Universität
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/210961441?language=en
关键词：
Regulation outer membrane biogenesis components

项目摘要

Cyanobacteria are photoautotrophic bacteria surrounded by an outer membrane in addition to the plasma membrane and the peptidoglycan layer. The biogenesis of this highly functional membrane is a complex phenomenon involving distinct machineries for the assembly of the outer membrane specific lipids and outer membrane proteins. The major outer membrane localized factors involved in this process are proteins of the polypeptide-transporting beta-barrel family and proteins for lipid A export. Both protein families found in cyanobacteria are phylogenetically related to the respective machineries involved in chloroplast biogenesis. We aim to understand the function and the interplay of these essential factors for outer membrane biogenesis in the cyanobacterium Anabaena sp. PCC 7120 as a cyanobacterial model system. We will explore the functional diversity of the three identified Omp85 proteins. This includes substrate definition as well as the analysis of the complexes formed by these three proteins. In parallel, we will analyze the complex architecture of the lipid A transport system as well as the functional mode of the transfer of the lipid molecule across the periplasm. The results will yield an understanding of the functional mode of the outer membrane, which is important for cell survival as it forms the outer barrier to the environment. In addition, our results will allow conclusions on the function of the proteins in chloroplasts, which contain both protein families in their outer membrane with function in protein and lipid transport.

蓝细菌是一种光合自养细菌，除了质膜和肽聚糖层外，还被一层外膜包围。这种高功能膜的生物发生是一种复杂的现象，涉及用于组装外膜特异性脂质和外膜蛋白的不同机制。参与这一过程的主要外膜定位因子是多肽转运β-桶家族的蛋白质和脂质A输出蛋白质。在蓝藻中发现的两种蛋白质家族与叶绿体生物发生中涉及的各自机制在遗传学上相关。我们的目的是了解的功能和这些重要因素的相互作用外膜生物合成的蓝藻鱼腥藻属PCC 7120作为蓝藻模型系统。我们将探讨三个确定Omp 85蛋白的功能多样性。这包括底物定义以及对这三种蛋白质形成的复合物的分析。同时，我们将分析脂质A转运系统的复杂结构以及脂质分子跨周质转运的功能模式。结果将产生外膜的功能模式的理解，这是重要的细胞生存，因为它形成了外部屏障的环境。此外，我们的研究结果将允许在叶绿体中的蛋白质的功能，其中包含在其外膜的蛋白质和脂质运输功能的两个蛋白质家族的结论。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Structure and Conservation of the Periplasmic Targeting Factor Tic22 Protein from Plants and Cyanobacteria*

植物和蓝细菌周质靶向因子 Tic22 蛋白的结构和保守*

DOI：
10.1074/jbc.m112.341644
发表时间：
2012
期刊：
The Journal of Biological Chemistry
影响因子：
0
作者：
Tripp J;Hahn A;Koenig P;Flinner N;Bublak D;Brouwer EM;Ertel F;Mirus O;Sinning I;Tews I;Schleiff E
通讯作者：
Schleiff E

LptC from Anabaena sp. PCC 7120: Expression, purification and crystallization.

来自鱼腥藻 PCC 7120 的 LptC：表达、纯化和结晶