Composition and perception of cytosolic complexes involved in precursor delivery to chloroplast membranes

胞质伴侣参与叶绿体功能所需的前体蛋白的转移。最近的观察对递送过程中的直接作用提出了挑战，因为发现了对前蛋白稳定性更直接的影响。这些原理只是基本的理解，必须探索才能理解前体转移到叶绿体的调节。在这个项目中，我们想要探索伴侣介导的降解是如何整合到细胞信号中的，以及它是如何与叶绿体的光合作用表现联系起来的。此外，我们的目的是分析mRNA分布和蛋白质易位之间的关系，这是一个主要讨论内质网和线粒体易位的主题，但尚未对叶绿体进行探索。

Cytosolic chaperones participate in the transfer of precursor proteins required for chloroplast function. Recent observations challenge the direct function in the delivery as a more direct influence on the preprotein stability is discovered. These principles are only rudimentary understood and have to be explored to understand the regulation of precursor transfer into chloroplasts. In this project we want to explore how the chaperone mediated degradation is integrated into the cellular signaling and how it is linked to photosynthetic performance of the chloroplasts. In addition, we aim at analyzing the relation between mRNA distribution and protein translocation, a theme largely discussed for the translocation into ER and mitochondria, but not yet explored for chloroplasts.