Modulation of ABA signaling by Ca2+-dependent posttranslational regulation

该项目旨在研究干旱激素脱落酸（ABA）对snf1相关激酶2 （SNRK2）激活的复杂调控网络，并鉴定SNRK2靶蛋白。总的来说，本项目主要关注磷酸化事件和蛋白-蛋白相互作用，以及与通过SNRK2激酶紧密控制信号传导的其他植物信号通路的相互作用。具体而言，该项目旨在：(a)通过上游事件分析SNRK2激酶的调控，包括CALCINEURIN B LIKE （CBL）蛋白和CBL相互作用蛋白激酶（CIPK）活性、茉莉酸信号传导和细菌效应物；(B)在蛋白质组水平上表征aba诱导的磷酸化事件，并通过比较激酶突变体和野生型植物的磷酸化模式来鉴定SNRK2激酶底物。该项目将有助于深入了解ABA与其他激素和病原体激活的信号通路在气孔调节中的复杂相互作用。此外，它将为非生物信号事件的动力学分析提供新的途径，并旨在确定病原体控制气孔调节事件的分子基础。

The proposed project aims to investigate the complex regulatory network that modulates activation of SNF1-RELATED KINASES 2 (SNRK2) in response to the drought hormone Abscisic acid (ABA) and to identify SNRK2 target proteins. In general, this project is focused on phosphorylation events and protein-protein interactions as well as interaction with other plant signaling pathways that tightly control signaling through SNRK2 kinases. Specifically this project aims to: (a) analyze the regulation of SNRK2 kinases via upstream events, including CALCINEURIN B LIKE (CBL) proteins and CBL-INTERACTING PROTEIN KINASE (CIPK) activity, Jasmonic acid signaling and bacterial effectors, (b) characterize ABA-induced phosphorylation events on a proteome level and identify SNRK2 kinase substrates by comparison of phosphorylation patterns in kinase mutants and wildtype plants. This project will generate insight into the complex interactions between ABA and other hormone and pathogen-activated signaling pathways in stomatal regulation. Moreover, it will provide a new approach to kinetic analysis of abiotic signaling events and aims to identify the molecular basis of pathogen control of stomatal regulation events.