Ethylene signaling of hypoxic stress adaptation in Arabidopsis

拟南芥缺氧应激适应的乙烯信号传导

• 批准号: 211162206
• 负责人: Professorin Dr. Margret Sauter (†)
• 金额: --
• 依托单位: Botanisches Institut und Botanischer Garten
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/211162206?language=en
• 关键词: Ethylene; signaling; hypoxic; stress; adaptation

Plants are aerobic organisms. Too little oxygen (hypoxia) renders energy supply difficult and results in impaired plant metabolism. Common causes for hypoxic or even anoxic (lack of oxygen) conditions in plants are soil water logging and submergence. Depending on their ecological niche, plants are more or less well adapted to cope with low oxygen stress. The gaseous hormone ethylene rapidly accumulates in plant tissues upon flooding. Ethylene controls many adaptations that improve plant survival during flooding. In the model plant Arabidopsis thaliana ethylene regulates hyponastic petiole growth which helps to move the leaf above the water surface. Ethylene signaling in concert with hypoxia signaling furthermore regulates metabolic adaptation to low oxygen stress. AP2/ERF (apetala2/ethylene response factor) transcription factors were identified as key mediators of plant responses to flooding. The Arabidopsis thaliana ERFs RAP2.2 and ERF73 were previously shown to be regulated by hypoxia and by ethylene and both increase tolerance to low oxygen stress when overexpressed. The proposed project aims to elucidate the contribution of ethylene signaling to hypoxia adaptation via RAP2.2 and ERF73 in Arabidopsis thaliana. To this end, we will clarify if RAP2.2 and ERF73 have overlapping and/or unique functions. We will identify genes that are targeted by these transcription factors at normoxic and hypoxic conditions or when exposed to ethylene. And we will ask if RAP2.2 and ERF73 mediate the full ethylene response in hypoxic stress adaptation in Arabidopsis. We expect that our study will provide a major contribution to our understanding of ethylene signaling of hypoxic stress tolerance.

植物是需氧生物。氧气太少（缺氧）会导致能量供应困难并导致植物新陈代谢受损。植物缺氧甚至缺氧（缺氧）状况的常见原因是土壤水涝和淹没。根据其生态位，植物或多或少能够很好地适应低氧胁迫。洪水过后，气态激素乙烯会在植物组织中迅速积聚。乙烯控制着许多提高植物在洪水期间生存的适应性。在模型植物拟南芥中，乙烯调节低叶柄生长，有助于将叶子移到水面以上。乙烯信号传导与缺氧信号传导进一步调节代谢适应低氧应激。 AP2/ERF（apetala2/乙烯响应因子）转录因子被确定为植物对洪水响应的关键介质。拟南芥 ERF RAP2.2 和 ERF73 先前已被证明受缺氧和乙烯调节，并且在过度表达时都会增加对低氧胁迫的耐受性。该项目旨在通过拟南芥中的 RAP2.2 和 ERF73 阐明乙烯信号传导对缺氧适应的贡献。为此，我们将澄清 RAP2.2 和 ERF73 是否具有重叠和/或独特的功能。我们将鉴定在常氧和低氧条件下或暴露于乙烯时这些转录因子靶向的基因。我们将询问 RAP2.2 和 ERF73 是否介导拟南芥低氧胁迫适应中的完整乙烯反应。我们期望我们的研究将为我们理解缺氧应激耐受的乙烯信号传导做出重大贡献。