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Analyzing rainforest ciliate diversity in Panama and Ecuador using nascent sequencing technologies and qRT-PCR.

使用新兴测序技术和 qRT-PCR 分析巴拿马和厄瓜多尔的雨林纤毛虫多样性。

基本信息

批准号：
211157732
负责人：
Dr. Micah Dunthorn, Ph.D.
金额：
--
依托单位：
Department of Biology
依托单位国家：
德国
项目类别：
Independent Junior Research Groups
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/211157732?language=en
关键词：
Analyzing rainforest ciliate diversity Panama

项目摘要

Communities of animal and plant species in Neotropical forests are known to be hyper-diverse, and species composition can change dramatically among communities because of varying levels of endemism. The aim here is to test if similar patterns of biodiversity and biogeography occur at the microbial level in the same forests. Soil ciliates in Panama and Ecuador will be used as models, and will be examined from multiple aspects using nascent sequencing and amplification technologies. 1) Deep sequencing of RNA over four years will allow for observations of total diversity, as well as any changes over distance and time. 2) Expression levels of RNA will be measured in soil ciliates to evaluate the appropriateness of using the RNA sequences from the Neotropical soils to estimate abundances. 3) Sexlinked genes will be inventoried in the putatively asexual colpodean ciliates to evaluate if their Neptropical soil sequences should be best compared to other sexual microbial and macro-organismic eukaryotic species, or to asexual strains of bacteria and archaea. Together these three aspects to observing soil ciliates and interpreting their sequences will help in establishing a microbial perspective that will broaden our view of the endangered Neotropical forests.

新热带森林的动植物物种群落是高度多样化的，由于地方特有程度的不同，群落之间的物种组成可能发生巨大变化。这里的目的是测试在同一森林的微生物水平上是否会出现类似的生物多样性和生物地理模式。巴拿马和厄瓜多尔的土壤纤毛虫将被用作模型，并将使用新兴的测序和扩增技术从多个方面进行研究。对RNA进行为期四年的深度测序，可以观察到总的多样性，以及随着距离和时间的变化。2)测定土壤纤毛虫RNA的表达水平，评价利用新热带土壤RNA序列估算其丰度的适宜性。3)对推测为无性生殖的阴头纤毛虫的性连锁基因进行盘点，以评估其热带土壤序列是否应该与其他有性生殖的微生物和大型真核生物物种，或与无性生殖的细菌和古细菌菌株进行比较。将这三个方面结合起来观察土壤纤毛虫并解释它们的序列将有助于建立一个微生物的视角，这将扩大我们对濒危新热带森林的看法。