EAGER: Multifunctional Nanobiointerfaces for Sensing Applications

Simonian（1317635）.该提案探索了一种使用CNT和酶进行生物反应传感的新方法，以在单个电极上实现级联层的LbL构造。以前，用于多分析物检测和多路复用的常用方法使用多电极阵列的组合，使用复杂的微流体、多点生物识别元件和通过繁重计算的信号处理。简化的方法应能提高效率、可靠性和扩大规模的潜力。在每一层存在不同的酶将提供识别级联反应下游的底物的独特能力。还可以基于每种酶的个体氧化电位的差异来检测中间产物，使得能够从一个电极同时检测和区分几种目标分析物。

Simonian(1317635). This proposal explores a new approach to cascading-reaction sensing using CNTs and enzymes to enable a LbL construction of the cascading layers on a single electrode. Previously, the common method for multianalyte detection and multiplexing used a combination of multi-electrode arrays, using complicated microfluidics, multi-spot biorecognition elements, and signal processing via heavy computing. The simplified approach should enable greater efficiencies, reliability, and scale-up potential. The presence of different enzymes at each layer will provide a unique capability to recognize substrates that are down-stream of cascade reactions. The intermediate products may also be detected, based on differences in individual oxidation potentials for each enzyme, enabling simultaneous detection and discrimination of several target analytes from one electrode.