SBIR Phase II: A Rapid Foodborne Pathogen Analyzer

该小型企业创新研究（SBIR）第二阶段项目建议开发一种原型食源性病原体分析仪，该分析仪将采用新型信用卡大小的采样设备，以所需的灵敏度（10 - 100 cfu/g）在1 - 2小时内提取，检测，识别和量化食品基质中特定病原体的存在。 目标是将菠菜中104 cfu/g鼠伤寒沙门氏菌在2小时内成功的I期测量延长至10-100 cfu/g S。设备和奶酪中的鼠伤寒沙门氏菌、哈密瓜中的单核细胞增生李斯特菌、绞细牛肉中的大肠杆菌O 157：H7以及家禽中的空肠弯曲杆菌。 食源性疾病每年影响美国多达5000万人，导致13万人住院治疗和3,000多人死亡。 不幸的是，目前用于检测这些病原体的方法依赖于耗时1 - 4天的漫长的生长富集步骤，从而无法有效预防受污染的食品分配和消费。如果成功，该项目更广泛的影响/商业潜力将是开发一种平台技术，在1 - 2小时内检测食品处理设备或食品中的病原体。 这将有利于食品行业提高生产力，最大限度地减少撤回和召回，最重要的是，最大限度地减少疾病爆发并可能挽救生命。 该分析仪占地面积小，可以在加工厂和支持实验室进行测量，最终可以在食品来源、港口和检查站进行测量。 它还可以加快确定爆发源的过程，帮助减少疾病和死亡。 通过开发拟议的采样系统获得的知识将允许开发类似的系统，可以监测供水中的病原体，检测空气中的生物制剂，以及医院患者中的传染性病原体（例如检测金黄色葡萄球菌，人类免疫缺陷病毒，结核分枝杆菌）。

This Small Business Innovation Research (SBIR) Phase II project proposes to develop a prototype foodborne pathogen analyzer that will employ a novel credit card sized sampling device to extract, detect, identify, and quantify the presence of specific pathogens in food matrices in 1 - 2 hours at the required sensitivity (10 - 100 cfu/g). The goal is to extend the successful Phase I measurements of 104 cfu/g Salmonella typhimurium in spinach within 2 hours to 10-100 cfu/g S. typhimurium on equipment and in cheese, Listeria monocytogenes in cantaloupe, Escherichia coli O157:H7 in ground beef, and Campylobacter jejuni in poultry. Foodborne diseases affect as many as 50 million people in the United States each year, resulting in 130,000 hospitalizations and over 3,000 deaths. Unfortunately, current methods used to detect these pathogens rely on lengthy growth enrichment steps that take 1 - 4 days, negating effective prevention of contaminated food distribution and consumption. The broader impact/commercial potential of this project, if successful, will be the development of a platform technology to detect pathogens on food handling equipment or in food in 1 - 2 hours. This will benefit the food industry by increasing productivity, minimizing withdrawals and recalls, and most importantly, minimizing illness outbreaks and potentially saving lives. The small footprint of the analyzer will allow measurements in process plants and supporting labs, and eventually, at food sources, ports, and inspection stations. It can also speed the process of identifying the source of an outbreak, helping minimize illnesses and deaths. The knowledge gained by developing the proposed sampling system will allow developing similar systems that can monitor pathogens in water supplies, detect bioagents in air, and infectious pathogens in hospital patients (e.g. detection of Staphylococcus aureus, human immunodeficiency virus, Mycobacterium tuberculosis).