MRI: Acquisition of Ion Torrent Personal Genome Machine to establish high-throughput sequencing capability for ecological and environmental biology

MRI：收购Ion Torrent个人基因组机，建立生态与环境生物学高通量测序能力

• 批准号: 1335772
• 负责人: Oleg Paliy
• 金额: $ 12.33万
• 依托单位: Wright State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1335772&HistoricalAwards=false
• 关键词: MRI; Acquisition; Ion; Torrent; Personal

An award is made to Wright State University to establish a next generation sequencing (NGS) capability at Wright State University based on the Ion Torrent Personal Genome Machine (PGM). Ion Torrent PGM Sequencer is a third-generation sequencer that is based on the use of a massively parallel array of proprietary semiconductor sensors to perform direct real-time measurements of the hydrogen ions produced during DNA replication. The unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high quality data at low costs. An unprecedented level of genetic information will thus be acquired in each experiment, which permits molecular studies in the areas of molecular ecology and genome investigation, where large amounts of nucleotide sequence data are required for a comprehensive analysis. Acquisition of this instrumentation will have an immediate and profound impact on the research at Wright State University. A variety of specific projects will be enabled through the establishment of the WSU NGS instrumentation that include microbial community structure determination, genome typing and sequencing, metagenomics, and gene expression.The broader impacts of this project focus on the integration of research, education, and student diversity. Directly, the acquisition of the requested equipment will greatly enhance the research infrastructure at Wright State University and will improve the quality of scientific research carried out at the university. NGS instrumentation will allow faculty to pursue research programs not possible with current resources, and will foster collaborative and interdisciplinary research efforts among participating faculty in the biological and biocomputational fields. Establishment of the NGS instrumentation at Wright State will also help the university expand its current graduate and undergraduate research and training programs and will help attract talented students to the university to participate in cutting edge research. NGS techniques will be incorporated into several graduate and undergraduate courses that will allow students to learn about high-throughput sequencing and get hands-on experience with this technology. New WSU NGS instrumentation will similarly allow many of the students with disabilities and those underrepresented in science, who are interested in obtaining research experience, to be trained in state-of-the-art techniques through several existing programs. The instrumentation will promote science to minorities and other underrepresented groups, and will make these students more competitive on the job market.

授予赖特州立大学一个奖项，以在赖特州立大学建立基于离子激流个人基因组机器（PGM）的下一代测序（NGS）能力。Ion Torrent PGM测序仪是第三代测序仪，基于使用专有半导体传感器的大规模并行阵列来对DNA复制过程中产生的氢离子进行直接实时测量。射流、微机械加工和半导体技术的独特组合使遗传信息（DNA）能够直接转化为数字信息（DNA序列），以低成本快速生成大量高质量数据。因此，在每次实验中将获得前所未有的遗传信息，这使得在分子生态学和基因组调查领域进行分子研究成为可能，因为在这些领域需要大量的核苷酸序列数据进行综合分析。获得这种仪器将对赖特州立大学的研究产生直接而深远的影响。通过建立WSU NGS仪器，将实现各种具体项目，包括微生物群落结构测定，基因组分型和测序，宏基因组学和基因表达。该项目的更广泛影响集中在研究，教育和学生多样性的整合上。直接而言，获得所要求的设备将大大加强赖特州立大学的研究基础设施，并将提高该大学开展的科学研究的质量。NGS仪器将允许教师追求研究计划不可能与当前的资源，并将促进参与生物和生物计算领域的教师之间的合作和跨学科的研究工作。在赖特州立大学建立NGS仪器也将有助于该大学扩大其目前的研究生和本科生研究和培训计划，并将有助于吸引有才华的学生到该大学参加尖端研究。NGS技术将被纳入几个研究生和本科生课程，这将使学生了解高通量测序，并获得这项技术的实践经验。新的WSU NGS仪器同样将允许许多残疾学生和那些在科学中代表性不足的学生，他们有兴趣获得研究经验，通过几个现有的方案接受最先进技术的培训。该仪器将向少数民族和其他代表性不足的群体推广科学，并将使这些学生在就业市场上更具竞争力。