MRI: Acquisition of a High Resolution LC-MS/MS System for Research and Education

MRI：获取用于研究和教育的高分辨率 LC-MS/MS 系统

• 批准号: 1337497
• 负责人: Christopher Mulligan
• 金额: $ 40万
• 依托单位: Board of Trustees of Illinois State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337497&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; LC; MS

With this award from the Chemistry Major Research Instrumentation Program, Professor Christopher Mulligan from Illinois State University (ISU) and colleagues Michael Byrns, T. Mitchell, Brian Brennan and Rachel Bowden will acquire a high resolution liquid chromatograph-tandem mass spectrometer (LC-MS/MS). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) embryonic regulation of the endocrine environment via the sulfotransferase-sulfatase pathway in an oviparous vertebrate; (b) detection of steroid hormones in treated sewage flowing through a constructed wetland; (c) natural products inspired organic synthesis; (d) peptidic and peptidomemetric therapeutics; (e) studies of the enzyme phosphocholine cytidylyltransferase; (f) molecular mechanisms regulating cytoskeleton organization in plant cells; (g) roles of microtubule-associated proteins in plant cell expansion; and (h) biomimetic studies of gold-based metallopharmaceuticals.Mass Spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with tandem capability provides additional structural identification power through further fragmentation of ions produced in the spectrometer. Several project personnel have secondary interests in chemical education research and, through on-site LC-MS/MS access, will develop discovery-based laboratory curricula incorporating concepts in proteomics and natural product-inspired organic synthesis. Extensive, hands-on experiences provided to undergraduates from this acquisition will better prepare these students for careers in science and further graduate studies. The ISU Department of Chemistry also regularly participates and directs nationally-funded undergraduate education initiatives. Examples of recent/current efforts include the nationally-recognized American Chemical Society Project SEED (Summer Experience for Economically Disadvantaged high school students) program, an NSF-funded Undergraduate Research Center, which provided summer research experiences for City Colleges of Chicago students with ISU Chemistry mentors, and faculty participation in the ISU chapter of the NSF-sponsored Lewis Stokes Alliance for Minority Participation (LSAMP) Program.

来自伊利诺伊州州立大学（ISU）的Christopher Mulligan教授和他的同事Michael Byrns，T。米切尔，布赖恩布伦南和雷切尔鲍登将获得一个高分辨率液相色谱串联质谱仪（LC-MS/MS）。该提案旨在加强各级的研究和教育，特别是在以下领域：（a）卵生脊椎动物通过磺基转移酶-硫酸酯酶途径对内分泌环境进行胚胎调节;（B）检测流经人工湿地的经处理污水中的类固醇激素;（c）天然产物激发的有机合成;（d）肽和肽计量疗法;（e）磷酸胆碱胞苷酰转移酶的研究;（f）调节植物细胞中细胞骨架组织的分子机制;（g）微管相关蛋白在植物细胞扩展中的作用;（h）金基金属药物的仿生研究。质谱（MS）是用于识别和表征嵌入在复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。具有串联能力的仪器通过进一步碎裂光谱仪中产生的离子提供额外的结构鉴定能力。几个项目人员在化学教育研究的次要利益，并通过现场LC-MS/MS访问，将开发发现为基础的实验室课程，结合蛋白质组学和天然产物启发的有机合成的概念。从这次收购提供给本科生的广泛的实践经验将更好地为这些学生在科学和进一步研究生学习的职业生涯做好准备。ISU化学系还定期参与和指导国家资助的本科教育计划。最近/目前的努力的例子包括国家认可的美国化学学会项目种子（夏季经验经济困难的高中生）计划，NSF资助的本科研究中心，提供夏季研究经验的芝加哥学生与ISU化学导师城市学院，并在NSF赞助的刘易斯斯托克斯联盟少数民族参与（LSAMP）计划ISU章教师参与。