MRI: HPLC/ESI-MS System for the Analysis of Strategic Chemical Species

该奖项由化学研究仪器和设施（CRIF）项目共同资助，来自北卡罗来纳大学夏洛特分校的Craig Ogle教授及其同事Olya Keen、John Daniels、Jerry Troutman和Pinku Mukherjee将获得高压液相色谱-串联质谱（HPLC/ESI-MS）系统和相关设备。该提案旨在加强各级的研究和教育，特别是在以下领域：(a)环境中微量有机污染物（troc）的量化；(b)表征糖聚合物生物合成系统的多糖产物和体内生产的类异戊二烯糖；(c)阐明和表征控制致癌信号通路的蛋白质-蛋白质相互作用；(d)阐明与赋予土壤和工业副产品疏水性有关的机制；(e)康乐用水的水质评估（游泳池、喷水台及水上公园）；(f) Ni（II）、Al（III）和Si（IV）与几种双吡啶吩嗪配体的合成配合物的表征；(g)使用低功率交流电和直流电（AC/DC）对有毒有机化学品进行电化学击穿；(h)偶联混合取代基卟啉化合物合成的卟啉二元体、聚合物和纳米材料的表征。质谱（MS）是用于鉴定和表征嵌入在复杂基质中的少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成母离子和母离子的碎片离子，并测量它们的质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。具有串联能力的仪器通过在光谱仪中产生的离子进一步破碎提供额外的结构识别能力。该奖项提供的仪器将为多个部门的教师和学生提供机会，使用该机构迄今未提供的现代仪器进行研究项目。拟议的仪器的可用性将增加现有的合作，并促进新的伙伴关系，这将使本科生和研究生接触到比他们原本经历的更多样化的研究环境，从而为他们提供更好地指导他们的科学抱负的机会。它将通过能源生产和基础设施中心（EPIC）和区域分析化学实验室（RACheL）的活动影响更广泛的社区，前者的任务是促进大学与企业的关系，后者为大夏洛特地区及其他地区的150多家客户公司进行化学分析。

With this award from the Chemistry Major Research Instrumentation Program that is co-funded by the Chemistry Research Instrumentation and Facilities (CRIF) Program, Professor Craig Ogle from University of North Carolina Charlotte and colleagues Olya Keen, John Daniels, Jerry Troutman and Pinku Mukherjee will acquire a high-pressure liquid chromatography-tandem mass spectrometry (HPLC/ESI-MS) system and associated equipment. The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) quantification of trace organic contaminants (TrOCs) in the environment; (b) characterization of polysaccharide products of sugar polymer biosynthesis systems and of isoprenoid-linked sugars produced in vivo; (c) elucidation and characterization of defined protein-protein interactions governing oncogenic signaling pathways; (d) elucidatation of the mechanisms associated with imparting hydrophobicity on soils and industrial byproducts; (e) evaluation of recreational water quality (in swimming pools, spray pads, and water parks); (f) characterization of synthesized complexes of Ni(II), Al(III), and Si(IV) with several dipyridophenazine ligands; (g) electrochemical breakdown of toxic organic chemicals using low power alternating and direct currents (AC/DC); and (h) characterization of porphyrin based dyads, polymers, and nanomaterials synthesized by coupling mixed-substituent porphyrin compounds.Mass Spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with tandem capability provides additional structural identification power through further fragmentation of ions produced in the spectrometer. The instrumentation provided by this award will provide faculty and students in several departments the opportunity to pursue research projects using modern instrumentation not heretofore available at the institution. Availability of the proposed instrumentation will increase existing collaborations and foster new partnerships that will expose undergraduate and graduate students to a more diverse research environment than they would have otherwise experienced, thus providing them a chance to better direct their scientific ambitions. It will impact the broader community through the activities of the Energy Production and Infrastructure Center (EPIC), which has a mandate to foster university-business relationships, and the Regional Analytical Chemistry Laboratory (RACheL), which carries out chemical analyses for over 150 client companies in the greater Charlotte region and beyond.