CSBR: Living Stocks: Species validation, enhanced data access and remote archiving of the Phaff Yeast Culture Collection

CSBR：活畜：Phaff 酵母菌种保藏的物种验证、增强的数据访问和远程存档

• 批准号: 1349395
• 负责人: Kyria Boundy-Mills
• 金额: $ 40.44万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1349395&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Species; validation

The Phaff Yeast Culture Collection, at the University of California Davis, is particularly useful for basic research such as yeast physiology, yeast ecology (especially yeast-insect associations), comparative genomics, and taxonomy including discovery of new yeast species. The Phaff collection of yeasts are also used for applied research such as food and beverage fermentation, food spoilage, biofuels exploration, and the development of improved methods to identify yeasts that cause human infections. Through the activities supported in this project undergraduate students will receive training in taxonomy and molecular biology. In addition, middle school students including underrepresented groups will learn about microbiology and culture collections through presentations in the UC Davis Bio Boot Camp. The Phaff Yeast Culture Collection is the fourth largest yeast collection in the world. It contains over 7,000 strains belonging to approximately 800 yeast species. Most strains are unique and not available from any other source. This project proposes a major overhaul of the collection to make it more useful, visible, and valuable to the worldwide yeast research and education community. This project will align the Phaff collection with comparable collections in Europe and Asia. Aims include: 1) Validate and update yeast species ID by DNA sequencing, 2) Upgrade to a more powerful database, website and online strain catalog, 3) Expand global accessibility by plugging into the Global Catalog of Microorganisms, GenBank and MycoBank, and 4) Complete the archival storage of yeasts at a remote cryopreservation facility. By posting DNA sequences of accessions in GenBank and expanding information available on the Phaff collection online catalog the collection will be more visible and valuable for all users. Lessons learned through this project will be shared with other US culture collection curators through the NSF-funded US Culture Collection Network. More information is publically available at: http://phaffcollection.ucdavis.edu.

加州大学戴维斯分校的法夫酵母培养物保藏中心对于酵母生理学、酵母生态学（特别是酵母-昆虫关联）、比较基因组学和分类学（包括发现新酵母物种）等基础研究特别有用。 Phaff 酵母系列还用于应用研究，例如食品和饮料发酵、食品腐败、生物燃料探索以及开发识别引起人类感染的酵母的改进方法。通过该项目支持的活动，本科生将接受分类学和分子生物学方面的培训。此外，包括代表性不足群体在内的中学生将通过加州大学戴维斯分校生物训练营的演示来了解微生物学和培养物收藏。 Phaff 酵母菌保藏中心是世界第四大酵母菌保藏中心。它包含大约 800 种酵母菌的 7,000 多个菌株。大多数菌株都是独特的，无法从任何其他来源获得。该项目提议对馆藏进行重大改革，使其对全球酵母研究和教育界更有用、更可见、更有价值。该项目将使 Phaff 系列与欧洲和亚洲的同类系列保持一致。目标包括：1) 通过 DNA 测序验证和更新酵母物种 ID，2) 升级到更强大的数据库、网站和在线菌株目录，3) 通过插入全球微生物目录、GenBank 和 MycoBank 扩大全球可访问性，4) 在远程低温保存设施中完成酵母的档案存储。通过在 GenBank 中发布收藏品的 DNA 序列并扩展 Phaff 馆藏在线目录上的可用信息，该馆藏对于所有用户来说将更加可见且更有价值。通过该项目获得的经验教训将通过 NSF 资助的美国菌种保藏网络与其他美国菌种保藏馆馆长分享。更多信息可公开访问：http://phaffcollection.ucdavis.edu。