CSBR: Living Stocks: The Escherichia Coli Genetic Stock Center

该奖项将有助于E。大肠杆菌遗传储备中心（CGSC）在耶鲁大学。大肠杆菌是一种重要的模式生物，从遗传学和生物化学的角度来看，它可以说是最完整的细胞生物。这种高度的理解使E.大肠杆菌不仅是一个重要的研究课题，也是研究各种生物的实验室主力，也是生物技术的宝贵工具。CGSC的使命是作为转基因大肠杆菌菌株的中央储存库。大肠杆菌中创建的，以便他们可以随时提供给其他研究人员和教育工作者。为此，CGSC保存了大约28，000个E的遗传定义衍生物培养物。大肠杆菌K-12。在该菌株集合中有超过8，000种不同的突变，在测序的E. coli K-12基因组。这些培养物被分发给美国和全球65个国家的研究人员和教育工作者。除了文化的分布，CGSC也是一个信息资源，并提供有关菌株，突变，基因，基因产物和遗传图谱的信息，在网上完全可搜索的数据库。重要的是，CGSC还为那些对使用E.大肠杆菌菌株的收集。CGSC将继续维护和扩大其关于菌株基因型、表型、历史和谱系的公开数据库，这是库存中心独有的。信息系统将得到加强，以增加与其他电子商务系统的兼容性。大肠杆菌网络资源，包括EcoliWiki项目，EcoGene和EcoCyc。CGSC正在与美国其他菌种保藏中心和世界微生物数据中心（WDCM）合作，以便这些菌种可以被全球菌株数据库索引，并创建国家微生物菌株数据库。在此期间，我们的新菌株构建服务的能力将扩大到那些需要转基因菌株但缺乏自行构建能力的用户，特别是传统细菌遗传学社区以外的用户。这是CGSC独有的服务，并显示出巨大的增长潜力。 访问应变收集数据库，包括基因型，基因功能和地图位置，以及广泛的参考书目可在线（cgsc.biology.yale.edu）。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

This award will contribute to the operation of the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is arguably the most completely understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The mission of the CGSC is to act as a central repository for genetically modified strains of E. coli created by the research community, so that they may be readily available to other researchers and educators. To that end, the CGSC maintains a collection of about 28,000 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in this strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators throughout the US and in over 65 countries worldwide. In addition to the distribution of cultures, the CGSC is also an information resource, and provides information about strains, mutations, genes, gene products and genetic maps, in a fully searchable database on the web. Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using the E. coli strains in the collection. The CGSC will continue to maintain and expand its publicly available database on strain genotypes, phenotypes, histories and pedigrees, which is unique to the Stock Center. The information systems will be enhanced to increase compatibility with other E. coli web resources, including the EcoliWiki project, EcoGene and EcoCyc. The CGSC is collaborating with other US culture collections and the World Data Centre for Microorganisms (WDCM) so that the collection can be indexed by global strain databases and to create a national microbial strain database. Over the period of this award, capabilities for our new strain construction services will be expanded for those users who require a genetically modified strain, but lack the capability of constructing it themselves, particularly to those outside of the traditional bacterial genetics community. This is a service unique to the CGSC and has shown great growth potential. Access to the strain collection database, including genotypes, gene function and map location, as well as an extensive bibliography are available online (cgsc.biology.yale.edu).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.