CSBR: Transfer of Ownership: Transfer of Lachance Yeast Collection to UC Davis

CSBR：所有权转让：将 Lachance 酵母收藏品转让给加州大学戴维斯分校

• 批准号: 2053253
• 负责人: Kyria Boundy-Mills
• 金额: $ 57.92万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2053253&HistoricalAwards=false
• 关键词: CSBR; Transfer; Ownership; Lachance; Yeast

The goal of this project is to transfer a large research collection of yeasts from Ontario, Canada to UC Davis. Yeasts (single-celled fungi) have great importance for life science research, industry, and natural ecosystems. They serve as degraders, nutrients, mutualists, competitors, parasites & pathogens. These important aspects of yeast ecology were elucidated in recent decades by several key researchers, including Herman Phaff (1913-2001, UC Davis) and Marc-André Lachance (University of Western Ontario). Lachance isolated, characterized, and preserved thousands of yeast strains between 1977 and 2014. He retired in June 2020 and seeks to transfer the collection to a public repository to make them available to the scientific community. Several aspects of this collection make it particularly valuable. It contains 330 type strains including the original type specimens of the 150 species that Lachance described, dozens of undescribed species, and strains that originated from 48 countries. Extensive characterization data was gathered. 5,000 yeast strains comprising the Lachance research collection will be transferred to the Phaff Yeast Culture Collection at the University of California Davis, the fourth largest of its kind in the world, with over 9,000 strains in the public catalog. This transfer will make the Lachance yeasts and associated data available to the research community for the first time, enabling use in a broad range of research fields such as taxonomy, ecology, physiology, comparative and functional genomics, and biogeography. Collection maintenance protocols of the Phaff Yeast Culture Collection follow relevant best practice guidelines to ensure the yeast strains meet the needs of the users: they are viable, pure, of the declared species, are backed up remotely, their characterization data are available to the user community, and handling, distribution and use comply with regulatory requirements such as biosafety, biosecurity, and Nagoya Protocol. Yeasts from the Lachance collection will therefore be accessioned into the Phaff collection under the collection’s standard protocols. Specifically, strains will be inventoried then shipped to UC Davis. Strains will be cryopreserved in triplicate: working and backup stocks at UC Davis, and a third copy cryopreserved off-site at the USDA National Laboratory for Genetic Resource Preservation in Fort Collins, Colorado. The viability, purity and species ID of each strain will be validated. Each strain will be identified by ribotyping using next generation sequencing by our collaborator Novozymes, Inc. at their research facility in Davis, CA. Strains that pass quality control criteria will be added to the Phaff collection public catalog and the Global Catalog of Microorganisms. DNA sequences will be deposited in GenBank. In addition to the already databased geographic and host material information, over 40 years of unpublished physiological data (up to 80 descriptors per strain) will be entered into the BioloMICS database and published in the Phaff collection online strain catalog. This multi-step process ensures that Lachance’s yeasts will continue to spark discoveries by future generations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目的目标是将加拿大安大略的大量酵母研究收集转移到加州大学戴维斯分校。酵母（单细胞真菌）在生命科学研究、工业和自然生态系统中具有重要意义。它们充当降解者、营养物、互惠者、竞争者、寄生虫和病原体。近几十年来，酵母生态学的这些重要方面被几位重要研究人员阐明，包括赫尔曼·法夫（1913-2001，加州大学戴维斯分校）和马克-安德烈·拉钱斯（西安大略大学）。Lachance在1977年至2014年间分离、鉴定和保存了数千种酵母菌株。他于2020年6月退休，并寻求将收藏品转移到公共存储库，以供科学界使用。这一系列的几个方面使它特别有价值。它包含330个模式菌株，包括Lachance描述的150个物种的原始模式标本，数十个未描述的物种，以及来自48个国家的菌株。收集了大量的表征数据。5,000酵母菌株组成的Lachance研究收集将被转移到法夫酵母培养物收集在加州戴维斯大学，第四大的同类在世界上，超过9,000株在公共目录。这一转让将使拉昌酵母和相关数据首次提供给研究界，使其能够用于广泛的研究领域，如分类学，生态学，生理学，比较和功能基因组学以及植物地理学。法夫酵母菌菌种保藏中心的菌种保藏维护方案遵循相关最佳实践指南，以确保酵母菌菌株满足用户的需求：它们是可存活的、纯净的、属于已申报的菌种、有远程备份、其特征数据可供用户社区使用，并且处理、分配和使用符合生物安全、生物安保和名古屋议定书等监管要求。因此，来自Lachance收集的酵母将根据收集的标准协议进入Phaff收集。具体而言，菌株将被库存，然后运往加州大学戴维斯分校。菌株将一式三份冷冻保存：工作和备用贮备液在UC Davis，第三份副本在柯林斯堡（科罗拉多）的USDA国家遗传资源保存实验室非现场冷冻保存。将验证每种菌株的活力、纯度和菌种ID。每种菌株将由我们的合作者Novozymes，Inc.使用下一代测序通过核糖分型进行鉴定。在加州戴维斯的研究机构通过质量控制标准的菌株将被添加到Phaff收集公共目录和全球微生物目录中。DNA序列将存入GenBank。除了已经建立数据库的地理和宿主材料信息外，超过40年未发表的生理数据（每个菌株最多80个描述符）将被输入BioloMICS数据库，并在Phaff collection在线菌株目录中发布。这个多步骤的过程确保了Lachance的酵母将继续激发后代的发现。这个奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。