CSBR: Transfer of Ownership: Transfer of Lachance Yeast Collection to UC Davis

CSBR:所有权转让:将 Lachance 酵母收藏品转让给加州大学戴维斯分校

基本信息

  • 批准号:
    2053253
  • 负责人:
  • 金额:
    $ 57.92万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-15 至 2024-07-31
  • 项目状态:
    已结题

项目摘要

The goal of this project is to transfer a large research collection of yeasts from Ontario, Canada to UC Davis. Yeasts (single-celled fungi) have great importance for life science research, industry, and natural ecosystems. They serve as degraders, nutrients, mutualists, competitors, parasites & pathogens. These important aspects of yeast ecology were elucidated in recent decades by several key researchers, including Herman Phaff (1913-2001, UC Davis) and Marc-André Lachance (University of Western Ontario). Lachance isolated, characterized, and preserved thousands of yeast strains between 1977 and 2014. He retired in June 2020 and seeks to transfer the collection to a public repository to make them available to the scientific community. Several aspects of this collection make it particularly valuable. It contains 330 type strains including the original type specimens of the 150 species that Lachance described, dozens of undescribed species, and strains that originated from 48 countries. Extensive characterization data was gathered. 5,000 yeast strains comprising the Lachance research collection will be transferred to the Phaff Yeast Culture Collection at the University of California Davis, the fourth largest of its kind in the world, with over 9,000 strains in the public catalog. This transfer will make the Lachance yeasts and associated data available to the research community for the first time, enabling use in a broad range of research fields such as taxonomy, ecology, physiology, comparative and functional genomics, and biogeography. Collection maintenance protocols of the Phaff Yeast Culture Collection follow relevant best practice guidelines to ensure the yeast strains meet the needs of the users: they are viable, pure, of the declared species, are backed up remotely, their characterization data are available to the user community, and handling, distribution and use comply with regulatory requirements such as biosafety, biosecurity, and Nagoya Protocol. Yeasts from the Lachance collection will therefore be accessioned into the Phaff collection under the collection’s standard protocols. Specifically, strains will be inventoried then shipped to UC Davis. Strains will be cryopreserved in triplicate: working and backup stocks at UC Davis, and a third copy cryopreserved off-site at the USDA National Laboratory for Genetic Resource Preservation in Fort Collins, Colorado. The viability, purity and species ID of each strain will be validated. Each strain will be identified by ribotyping using next generation sequencing by our collaborator Novozymes, Inc. at their research facility in Davis, CA. Strains that pass quality control criteria will be added to the Phaff collection public catalog and the Global Catalog of Microorganisms. DNA sequences will be deposited in GenBank. In addition to the already databased geographic and host material information, over 40 years of unpublished physiological data (up to 80 descriptors per strain) will be entered into the BioloMICS database and published in the Phaff collection online strain catalog. This multi-step process ensures that Lachance’s yeasts will continue to spark discoveries by future generations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
这个项目的目标是将大量的酵母研究收集从加拿大安大略省转移到加州大学戴维斯分校。酵母(单细胞真菌)在生命科学研究、工业和自然生态系统中具有重要意义。它们是降解者、营养物、共生者、竞争者、寄生虫和病原体。最近几十年,包括Herman Phaff(1913-2001,加州大学戴维斯分校)和marc - andr<s:1> Lachance(西安大略大学)在内的几位重要研究人员阐明了酵母生态学的这些重要方面。1977年至2014年间,Lachance分离、鉴定并保存了数千株酵母菌。他于2020年6月退休,并试图将这些藏品转移到一个公共存储库,以便向科学界提供。这个收藏品的几个方面使它特别有价值。它包含330种类型菌株,包括Lachance描述的150种的原始类型标本,数十种未描述的物种以及源自48个国家的菌株。收集了大量的表征数据。Lachance研究收藏的5000株酵母菌将被转移到加州大学戴维斯分校的Phaff酵母菌培养收藏,这是世界上第四大酵母菌培养收藏,公共目录中有9000多株酵母菌。这一转移将使Lachance酵母和相关数据首次提供给研究界,使其能够用于广泛的研究领域,如分类学、生态学、生理学、比较和功能基因组学以及生物地理学。法夫酵母菌培养试剂盒的收集维护方案遵循相关的最佳实践指南,以确保酵母菌株满足用户的需求:它们是有活力的、纯的、申报的品种,远程备份,其特性数据可供用户社区使用,处理、分发和使用符合生物安全、生物安保和名古屋议定书等监管要求。因此,根据收集的标准协议,Lachance收集的酵母将加入Phaff收集。具体来说,菌株将被清点,然后运往加州大学戴维斯分校。菌株将被冷冻保存为三份:工作和备用库存在加州大学戴维斯分校,第三份副本在科罗拉多州柯林斯堡的美国农业部遗传资源保存国家实验室冷冻保存。对每个菌株的生存力、纯度和菌种ID进行验证。每个菌株将由我们的合作伙伴Novozymes, Inc.在其位于加利福尼亚州戴维斯的研究设施通过下一代测序进行核糖分型鉴定。通过质量控制标准的菌株将被添加到Phaff收集公共目录和全球微生物目录中。DNA序列将存入GenBank。除了已经建立的地理和宿主材料信息外,超过40年未发表的生理数据(每个菌株多达80个描述符)将被输入BioloMICS数据库并发表在Phaff馆藏在线菌株目录中。这个多步骤的过程确保了Lachance的酵母将继续激发后代的发现。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

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Kyria Boundy-Mills其他文献

難培養性微生物研究の最新技術III ―微生物の生き様に迫り課題解決へ― 第12章「昆虫と酵母の共生」
难培养微生物研究最新技术Ⅲ——走近微生物生存方式,解决问题——第十二章“昆虫与酵母的共生”
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Marizeth Groenewald;Kyria Boundy-Mills;Neza Cadez;Rikiya Endoh;Sasitorn Jindamorakot;Carolina Pohl-Albertyn;Carlos A. Rosa;Benedetta Turchetti;Andrey Yurkov;遠藤 力也
  • 通讯作者:
    遠藤 力也
Chapter 15: Census of Yeasts Isolated from Natural Ecosystem and Conserved in Worldwide Collections. In: Yeasts in Natural Ecosystems: Diversity.
第 15 章:从自然生态系统中分离出来并在全球收藏中保存的酵母的普查。
  • DOI:
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Marizeth Groenewald;Kyria Boundy-Mills;Neza Cadez;Rikiya Endoh;Sasitorn Jindamorakot;Carolina Pohl-Albertyn;Carlos A. Rosa;Benedetta Turchetti;Andrey Yurkov
  • 通讯作者:
    Andrey Yurkov

Kyria Boundy-Mills的其他文献

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{{ truncateString('Kyria Boundy-Mills', 18)}}的其他基金

CSBR: Ownership Transfer: Rescue of two orphaned yeast collections
CSBR:所有权转让:拯救两个孤立的酵母收藏品
  • 批准号:
    1561580
  • 财政年份:
    2016
  • 资助金额:
    $ 57.92万
  • 项目类别:
    Continuing Grant
CSBR: Living Stocks: Species validation, enhanced data access and remote archiving of the Phaff Yeast Culture Collection
CSBR:活畜:Phaff 酵母菌种保藏的物种验证、增强的数据访问和远程存档
  • 批准号:
    1349395
  • 财政年份:
    2014
  • 资助金额:
    $ 57.92万
  • 项目类别:
    Continuing Grant

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  • 批准号:
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  • 批准年份:
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合作研究:CSBR:所有权转让:活畜:丛枝菌根真菌国际菌种保藏中心 (INVAM)
  • 批准号:
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Collaborative Research: CSBR: Ownership Transfer: Living Stocks: International Culture Collection of Arbuscular Mycorrhizal Fungi (INVAM)
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