CSBR: Transfer of Ownership: Transfer of Lachance Yeast Collection to UC Davis

CSBR：所有权转让：将 Lachance 酵母收藏品转让给加州大学戴维斯分校

• 批准号: 2053253
• 负责人: Kyria Boundy-Mills
• 金额: $ 57.92万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2053253&HistoricalAwards=false
• 关键词: CSBR; Transfer; Ownership; Lachance; Yeast

The goal of this project is to transfer a large research collection of yeasts from Ontario, Canada to UC Davis. Yeasts (single-celled fungi) have great importance for life science research, industry, and natural ecosystems. They serve as degraders, nutrients, mutualists, competitors, parasites & pathogens. These important aspects of yeast ecology were elucidated in recent decades by several key researchers, including Herman Phaff (1913-2001, UC Davis) and Marc-André Lachance (University of Western Ontario). Lachance isolated, characterized, and preserved thousands of yeast strains between 1977 and 2014. He retired in June 2020 and seeks to transfer the collection to a public repository to make them available to the scientific community. Several aspects of this collection make it particularly valuable. It contains 330 type strains including the original type specimens of the 150 species that Lachance described, dozens of undescribed species, and strains that originated from 48 countries. Extensive characterization data was gathered. 5,000 yeast strains comprising the Lachance research collection will be transferred to the Phaff Yeast Culture Collection at the University of California Davis, the fourth largest of its kind in the world, with over 9,000 strains in the public catalog. This transfer will make the Lachance yeasts and associated data available to the research community for the first time, enabling use in a broad range of research fields such as taxonomy, ecology, physiology, comparative and functional genomics, and biogeography. Collection maintenance protocols of the Phaff Yeast Culture Collection follow relevant best practice guidelines to ensure the yeast strains meet the needs of the users: they are viable, pure, of the declared species, are backed up remotely, their characterization data are available to the user community, and handling, distribution and use comply with regulatory requirements such as biosafety, biosecurity, and Nagoya Protocol. Yeasts from the Lachance collection will therefore be accessioned into the Phaff collection under the collection’s standard protocols. Specifically, strains will be inventoried then shipped to UC Davis. Strains will be cryopreserved in triplicate: working and backup stocks at UC Davis, and a third copy cryopreserved off-site at the USDA National Laboratory for Genetic Resource Preservation in Fort Collins, Colorado. The viability, purity and species ID of each strain will be validated. Each strain will be identified by ribotyping using next generation sequencing by our collaborator Novozymes, Inc. at their research facility in Davis, CA. Strains that pass quality control criteria will be added to the Phaff collection public catalog and the Global Catalog of Microorganisms. DNA sequences will be deposited in GenBank. In addition to the already databased geographic and host material information, over 40 years of unpublished physiological data (up to 80 descriptors per strain) will be entered into the BioloMICS database and published in the Phaff collection online strain catalog. This multi-step process ensures that Lachance’s yeasts will continue to spark discoveries by future generations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目的目标是将大量酵母研究收藏品从加拿大安大略省转移到加州大学戴维斯分校。酵母（单细胞真菌）对于生命科学研究、工业和自然生态系统具有重要意义。它们充当降解者、营养素、互利共生者、竞争者、寄生虫和病原体。近几十年来，包括 Herman Phaff（1913-2001，加州大学戴维斯分校）和 Marc-André Lachance（西安大略大学）在内的几位关键研究人员阐明了酵母生态学的这些重要方面。 Lachance 在 1977 年至 2014 年间分离、表征并保存了数千种酵母菌株。他于 2020 年 6 月退休，并寻求将这些菌株转移到公共存储库，以便科学界可以使用它们。该藏品的几个方面使其特别有价值。它包含 330 个模式菌株，包括 Lachance 描述的 150 个物种的原始模式标本、数十个未描述的物种以及源自 48 个国家的菌株。收集了广泛的表征数据。 Lachance 研究保藏中的 5,000 株酵母菌株将被转移到加州大学戴维斯分校的 Phaff 酵母培养物保藏中心，这是世界上第四大酵母菌保藏中心，公共目录中有 9,000 多个菌株。此次转让将使 Lachance 酵母菌和相关数据首次向研究界开放，从而能够在分类学、生态学、生理学、比较和功能基因组学以及生物地理学等广泛的研究领域中使用。 Phaff酵母培养物保藏中心的保藏维护方案遵循相关的最佳实践指南，以确保酵母菌株满足用户的需求：它们是申报物种的活菌、纯品、远程备份、其特征数据可供用户社区使用，并且处理、分发和使用符合生物安全、生物保安和名古屋协议等监管要求。因此，Lachance 收藏品中的酵母将根据该收藏品的标准方案加入 Phaff 收藏品中。具体来说，菌株将被清点，然后运往加州大学戴维斯分校。菌株将一式三份冷冻保存：工作库存和备用库存在加州大学戴维斯分校，第三份副本在位于科罗拉多州柯林斯堡的美国农业部国家遗传资源保存实验室进行异地冷冻保存。每个菌株的活力、纯度和物种 ID 将得到验证。我们的合作者 Novozymes, Inc. 将在其位于加利福尼亚州戴维斯的研究机构中使用下一代测序技术通过核糖分型来鉴定每种菌株。通过质量控制标准的菌株将被添加到 Phaff 收藏公共目录和全球微生物目录中。 DNA序列将存入GenBank。除了已经数据库化的地理和宿主材料信息外，超过 40 年未发表的生理数据（每个菌株最多 80 个描述符）将被输入 BioloMICS 数据库，并在 Phaff 收集在线菌株目录中发布。这一多步骤过程确保 Lachance 的酵母将继续激发子孙后代的发现。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。