CSBR: Transfer of Ownership: Transfer of Lachance Yeast Collection to UC Davis

该项目的目标是将加拿大安大略省的大量酵母研究集合转移到加州大学戴维斯分校。酵母(单细胞真菌)对生命科学研究、工业和自然生态系统具有重要意义。它们是降解者、营养物、互惠者、竞争者、寄生虫和病原体。近几十年来，包括Herman Phaff(1913-2001，加州大学戴维斯分校)和Marc-AndréLachance(西安大略大学)在内的几位关键研究人员阐明了酵母生态的这些重要方面。在1977至2014年间，Lachance分离、鉴定和保存了数千株酵母菌株。他于2020年6月退休，并寻求将这些藏品转移到公共储存库，供科学界使用。这个收藏品的几个方面使它特别有价值。它包含330个模式菌株，包括Lachance描述的150个物种的原始模式标本，数十个未描述的物种，以及来自48个国家的菌株。收集了大量的表征数据。包括Lachance研究收藏的5,000个酵母菌株将被转移到加州大学戴维斯分校的Phaff酵母培养收藏中，这是世界上第四大酵母培养收藏，公共目录中有超过9,000个菌株。这项转移将首次向研究界提供Lachance酵母和相关数据，使其能够在广泛的研究领域使用，如分类学、生态学、生理学、比较和功能基因组学以及生物地理学。Phaff酵母培养物品库的收集维护协议遵循相关的最佳实践指南，以确保酵母菌株满足用户的需求：它们是有活力的、纯净的、符合申报物种的、远程备份的、其特征数据可供用户社区使用，并且处理、分配和使用符合法规要求，如生物安全、生物安全和名古屋协议。因此，来自Lachance集合的酵母将根据集合的标准协议被加入Phaff集合。具体来说，菌株将被清点，然后运往加州大学戴维斯分校。菌株将被冷冻保存一式三份：工作库存和备份库存在加州大学戴维斯分校，第三份在科罗拉多州科罗拉多州柯林斯堡的美国农业部遗传资源保存国家实验室异地冷冻保存。将对每个菌株的活力、纯度和物种ID进行验证。我们的合作者Novozymes，Inc.将在他们位于加利福尼亚州戴维斯的研究机构通过核糖体测序来鉴定每个菌株。通过质量控制标准的菌株将被添加到Phaff集合公共目录和全球微生物目录中。DNA序列将保存在GenBank中。除了已经建立数据库的地理和宿主材料信息外，40多年来未公布的生理数据(每个菌株最多80个描述符)将被输入BioloMICS数据库，并在Phaff收集的在线菌株目录中发布。这一多步骤的过程确保了Lachance的酵母将继续激发未来几代人的发现。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The goal of this project is to transfer a large research collection of yeasts from Ontario, Canada to UC Davis. Yeasts (single-celled fungi) have great importance for life science research, industry, and natural ecosystems. They serve as degraders, nutrients, mutualists, competitors, parasites & pathogens. These important aspects of yeast ecology were elucidated in recent decades by several key researchers, including Herman Phaff (1913-2001, UC Davis) and Marc-André Lachance (University of Western Ontario). Lachance isolated, characterized, and preserved thousands of yeast strains between 1977 and 2014. He retired in June 2020 and seeks to transfer the collection to a public repository to make them available to the scientific community. Several aspects of this collection make it particularly valuable. It contains 330 type strains including the original type specimens of the 150 species that Lachance described, dozens of undescribed species, and strains that originated from 48 countries. Extensive characterization data was gathered. 5,000 yeast strains comprising the Lachance research collection will be transferred to the Phaff Yeast Culture Collection at the University of California Davis, the fourth largest of its kind in the world, with over 9,000 strains in the public catalog. This transfer will make the Lachance yeasts and associated data available to the research community for the first time, enabling use in a broad range of research fields such as taxonomy, ecology, physiology, comparative and functional genomics, and biogeography. Collection maintenance protocols of the Phaff Yeast Culture Collection follow relevant best practice guidelines to ensure the yeast strains meet the needs of the users: they are viable, pure, of the declared species, are backed up remotely, their characterization data are available to the user community, and handling, distribution and use comply with regulatory requirements such as biosafety, biosecurity, and Nagoya Protocol. Yeasts from the Lachance collection will therefore be accessioned into the Phaff collection under the collection’s standard protocols. Specifically, strains will be inventoried then shipped to UC Davis. Strains will be cryopreserved in triplicate: working and backup stocks at UC Davis, and a third copy cryopreserved off-site at the USDA National Laboratory for Genetic Resource Preservation in Fort Collins, Colorado. The viability, purity and species ID of each strain will be validated. Each strain will be identified by ribotyping using next generation sequencing by our collaborator Novozymes, Inc. at their research facility in Davis, CA. Strains that pass quality control criteria will be added to the Phaff collection public catalog and the Global Catalog of Microorganisms. DNA sequences will be deposited in GenBank. In addition to the already databased geographic and host material information, over 40 years of unpublished physiological data (up to 80 descriptors per strain) will be entered into the BioloMICS database and published in the Phaff collection online strain catalog. This multi-step process ensures that Lachance’s yeasts will continue to spark discoveries by future generations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.