CSBR: Living Stocks - Enhancement of the Caenorhabditis Natural Diversity Resource

CSBR：活畜 - 增强秀丽隐杆线虫自然多样性资源

• 批准号: 1930382
• 负责人: Erik Andersen
• 金额: $ 70.81万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1930382&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Enhancement; Caenorhabditis

Studies of conserved biological processes require model organisms which share attributes with humans. Most model organisms are easily grown in the laboratory and enable experimental manipulations. Over the last forty years, the nematode Caenorhabditis elegans has been instrumental in numerous fundamental advances in genetics, developmental biology, and neurobiology. However, all of these discoveries come from studies of a single strain in the species. This limitation is similar to the argument that we can understand all of human biology by the study of a single person in our species. This project seeks to expand the C. elegans Natural Diversity Resource (CeNDR), which comprises nearly 1000 genetically distinct strains from around the world. This genetic diversity facilitates discoveries about the evolution of physiological and behavioral traits, including responses to chemical and biotic stresses. Because humans share many of the same genes and processes as this nematode species, studies using this organism can teach us a great deal about how the human population differs.CeNDR has three major goals: (1) Collect, organize, and disseminate wild C. elegans strains; (2) Organize and disseminate whole-genome sequence and variant data for wild strains; and (3) Provide a genetics portal to facilitate mappings and interpretations of results. This project will address critical improvements to the methodology and technology of each of those goals. These improvements will enable future discoveries with CeNDR and will enable the planned expansion to include strains, whole-genome data, and mappings for two related nematode species: C. briggsae and C. tropicalis. This expansion to the Caenorhabditis Natural Diversity Resource (CaeNDR) will build unparalleled new capacity for comparative evolution approaches in nematodes and likely other organisms. Most research groups do not have the combination of experimental, computational, and statistical training to easily perform studies of natural variation. This project will make these tools available and usable by research laboratories across diverse model organism communities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

保守的生物过程的研究需要与人类共享属性的模式生物。 大多数模式生物很容易在实验室中生长，并能够进行实验操作。 在过去的40年里，秀丽隐杆线虫在遗传学、发育生物学和神经生物学的许多基础性进展中发挥了重要作用。 然而，所有这些发现都来自对该物种中单一菌株的研究。 这种局限性类似于我们可以通过研究我们物种中的一个人来理解所有人类生物学的论点。 该项目旨在扩展C。线虫自然多样性资源（CeNDR），包括来自世界各地的近1000种遗传上不同的菌株。 这种遗传多样性有助于发现生理和行为特征的进化，包括对化学和生物应激的反应。 由于人类与这种线虫有许多相同的基因和过程，使用这种生物体的研究可以告诉我们很多关于人类种群如何不同的信息。elegans菌株;（2）整理和传播野生菌株的全基因组序列和变异数据;以及（3）提供遗传学门户网站，以促进绘制和解释结果。 该项目将解决对每个目标的方法和技术的重大改进。 这些改进将使CeNDR的未来发现成为可能，并将使计划的扩展包括菌株，全基因组数据和两个相关线虫物种的映射：C。briggsae和C.热带植物 这种对小杆线虫自然多样性资源（CaeNDR）的扩展将为线虫和其他可能的生物体的比较进化方法建立无与伦比的新能力。 大多数研究小组没有实验，计算和统计训练的组合，可以轻松地进行自然变异的研究。 该项目将使这些工具可供不同模式生物社区的研究实验室使用。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。

项目成果

Evaluating the power and limitations of genome-wide association studies in Caenorhabditis elegans.

• DOI: 10.1093/g3journal/jkac114
• 发表时间: 2022-07-06
• 期刊: G3 (Bethesda, Md.)