CAREER: Effects of nutrients on antibiotic resistance and antibiotic subsistence

职业：营养物质对抗生素耐药性和抗生素生存的影响

• 批准号: 1351676
• 负责人: Xu Li
• 金额: $ 40万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1351676&HistoricalAwards=false
• 关键词: CAREER; Effects; nutrients; antibiotic; resistance

1351676LiMany people have become aware of the issue of pharmaceuticals in water and wastewater and natural waters. With the pharmaceutical market worldwide estimated to be $1 trillion dollars in 2015 and with an aging population (which uses more pharmaceuticals) and water and wastewater treatment plants never designed to deal with extremely low concentrations of highly biologically active compounds, the problem will likely get worse. One of the biggest, yet very subtle is the issue of antibiotic resistance in bacteria in natural waters.Antibiotic resistance constitutes a national and global public health threat. Evidence suggests that in addition to coming from hospitals, antibiotic resistant bacteria also emerge in the environment due to prolonged exposure to antibiotics originating in livestock and human wastes. On the other hand, many antibiotic resistant bacteria can subsist on antibiotics by using them as carbon and energy sources. Because nutrients influence microbial metabolisms and vary in quantity and composition across environmental compartments, they represent an important environmental factor that can affect antibiotic resistance and antibiotic subsistence in the environment. However, how nutrients affect the emergence of antibiotic resistance and the occurrence of antibiotic subsistence in microbial communities is poorly understood. The overall goal of this project is to build an integrated research and education/outreach program to minimize the negative impacts of antibiotics and antibiotic resistance to environmental and public health. The research goal of the project is to understand the interactions between antibiotics and microbial (bacterial) communities under various nutrient levels and types (i.e., labile vs. recalcitrant). The research approach will investigate the responses of bacteria to both nutrient starvation and antibiotic presence. The results of these studies will allow implementation in municipal and agricultural wastewater treatment to control the emergence and proliferation of antibiotic resistant bacteria. The proposed project will use a novel analytical tool, quantitative (meta)proteomics, which can offer new and valuable perspectives to environmental engineering studies. The use of quantitative (meta)proteomics in the mechanistic studies on antibiotic resistance and antibiotic subsistence will lay the groundwork for exploring further application of this novel technology in other environmental engineering fields such as exposure and biodegradation studies.One major source of antibiotics in the environment is livestock manure. The education goal of the project is to promote behavioral changes in livestock producers on antibiotic administration and manure management and to educate a new generation of environmental engineers specializing in solving agriculture-related environmental challenges. This will be accomplished by:1.) Raising the awareness of antibiotics and antibiotic resistance as environmental contaminants among livestock producers through outreach and education activities2.) Develop an integrated course module to stimulate interest in environmental science among high school students in rural areas, and,3.) Encourage rural students to pursue a graduate degree in environmental engineering

[1351676]许多人已经意识到水、废水和天然水中的药物问题。据估计，2015年全球制药市场将达到1万亿美元，随着人口老龄化（使用更多的药物），以及水和废水处理厂从未设计过处理极低浓度的高生物活性化合物，问题可能会变得更糟。其中一个最大但又非常微妙的问题是天然水体中细菌的抗生素耐药性问题。抗生素耐药性对国家和全球公共卫生构成威胁。有证据表明，除了来自医院之外，耐抗生素细菌也由于长期接触源自牲畜和人类废物的抗生素而出现在环境中。另一方面，许多耐药细菌可以利用抗生素作为碳和能源来生存。由于营养物质影响微生物代谢，并且在不同的环境隔间中数量和组成不同，因此它们是影响抗生素耐药性和抗生素在环境中的生存的重要环境因素。然而，营养物质如何影响抗生素耐药性的出现和微生物群落中抗生素生存的发生尚不清楚。该项目的总体目标是建立一个综合研究和教育/推广计划，以尽量减少抗生素和抗生素耐药性对环境和公共卫生的负面影响。该项目的研究目标是了解不同营养水平和类型（即不稳定与顽固）下抗生素与微生物（细菌）群落之间的相互作用。该研究方法将研究细菌对营养饥饿和抗生素存在的反应。这些研究的结果将允许在城市和农业废水处理中实施，以控制抗生素耐药细菌的出现和增殖。该项目将使用一种新的分析工具，定量（元）蛋白质组学，它可以为环境工程研究提供新的和有价值的视角。定量（元）蛋白质组学在抗生素耐药性和抗生素生存机制研究中的应用将为进一步探索该新技术在其他环境工程领域如暴露和生物降解研究中的应用奠定基础。环境中抗生素的一个主要来源是牲畜粪便。该项目的教育目标是促进牲畜生产者在抗生素使用和粪便管理方面的行为改变，并培养新一代环境工程师，专门解决与农业有关的环境挑战。这将通过以下方式实现：通过外联和教育活动，提高牲畜生产者对抗生素和抗生素耐药性作为环境污染物的认识。2 .开发一个综合课程模块，以激发农村地区高中生对环境科学的兴趣；鼓励农村学生攻读环境工程研究生学位

项目成果

Predicting antibiotic resistance gene abundance in activated sludge using shotgun metagenomics and machine learning

使用鸟枪宏基因组学和机器学习预测活性污泥中抗生素抗性基因的丰度

• DOI: 10.1016/j.watres.2021.117384
• 发表时间: 2021
• 期刊: Water Research
• 影响因子: 12.8
• 作者: Sun, Yuepeng;Clarke, Bertrand;Clarke, Jennifer;Li, Xu
• 通讯作者: Li, Xu