Dairy bioactive nutrients synergistic effects against inflammation: nutrigenomics and metabolomics approaches

乳制品生物活性营养素对抗炎症的协同作用：营养基因组学和代谢组学方法

• 批准号: RGPIN-2016-05999
• 负责人: Rudkowska, Iwona
• 金额: $ 1.82万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680416
• 关键词: Dairy; bioactive; nutrients; synergistic; effects

Current evidences support a benefit for dairy bioactive nutrients against inflammatory reactions. Although the specific mechanisms underlying these effects have not been fully elucidated, convincing evidences support the hypothesis that dairy components, such as fat and proteins, actively impact the release of pro-inflammatory molecules. Further, earlier mechanistic studies have investigated the beneficial effects of individual dairy fat and proteins on inflammation. However, the possibility that these bioactive molecules synergise together has never been studied. The long-term objective of the proposed research program is to characterize the combined actions of novel bioactive nutrients on inflammation in mammals and to define the required food enrichment. The next five-year objective is to determine whether macronutrient components, present specifically in dairy fats and proteins/amino acids, can individually, additively or synergistically, regulate inflammatory reactions. We will use genomics and metabolomics technologies to identify the molecular signatures and the mechanism of action of components of dairy products on inflammation indicators in cultured cells. Based on our pilot work, this research initiative may lead to the identification of highly protective fat/protein combination(s), and we anticipate that future directions will involve in vivo validations steps. Together, these in vitro studies will help to better understand the mechanisms of action of dairy bioactive molecules on inflammatory reactions. The targeted suppression of various pro-inflammatory cytokines in relevant tissues represents an exciting new opportunity for supplements or functional ingredients for animal and human intake. Further, the use of innovative OMICs technologies will increase our knowledge on the beneficial molecular effects of bioactive molecules. Taken together, this NSERC program will allow us to bring innovative and competitive products with optimal combination of bioactive components to the marketplace. Such a research program will promote the development and expansion of research in the field of nutrigenomics and food science in the country and will enhance the training of HQPs who will go on to positively impact the Canadian economy and Canadian-based research and discovery.

目前的证据支持乳制品生物活性营养素对炎症反应的益处。尽管这些影响的具体机制尚未完全阐明，但令人信服的证据支持乳制品成分（如脂肪和蛋白质）积极影响促炎分子释放的假设。此外，早期的机制研究已经调查了个别乳制品脂肪和蛋白质对炎症的有益作用。然而，这些生物活性分子协同作用的可能性从未被研究过。拟议研究计划的长期目标是表征新型生物活性营养素对哺乳动物炎症的联合作用，并确定所需的食物富集。下一个五年的目标是确定常量营养素成分，特别是存在于乳制品脂肪和蛋白质/氨基酸中，是否可以单独、加和或协同调节炎症反应。我们将使用基因组学和代谢组学技术来鉴定乳制品成分对培养细胞炎症指标的分子特征和作用机制。基于我们的试点工作，这项研究计划可能会导致高度保护性的脂肪/蛋白质组合的鉴定，我们预计未来的方向将涉及体内验证步骤。总之，这些体外研究将有助于更好地了解乳制品生物活性分子对炎症反应的作用机制。对相关组织中各种促炎细胞因子的靶向抑制为动物和人类摄入的补充剂或功能性成分提供了令人兴奋的新机会。此外，使用创新的组学技术将增加我们对生物活性分子有益分子效应的认识。总的来说，这个NSERC项目将使我们能够将具有最佳生物活性成分组合的创新和有竞争力的产品推向市场。这样一个研究项目将促进加拿大营养基因组学和食品科学领域研究的发展和扩大，并将加强对hqp的培训，这些hqp将继续对加拿大经济和加拿大的研究和发现产生积极影响。