Antibiotic Resistance Genes in the Soil-Plant Ecosystem

土壤-植物生态系统中的抗生素抗性基因

• 批准号: 1805990
• 负责人: Xu Li
• 金额: $ 33万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1805990&HistoricalAwards=false
• 关键词: Antibiotic; Resistance; Genes; Soil; Plant

Antibiotic resistance genes (ARGs) make bacteria resistant to antibiotics, and disease-causing pathogens possessing ARGs can make antibiotic treatment of infectious deceases less effective in treating pathogens, and hence, threaten public health. Soil is a major environmental reservoir of ARGs, and edible crops grown in ARG-containing soils have the potential to serve as a carrier of ARGs to humans and livestock that feed on the crops. After being introduced to the soil in croplands, some ARG-carrying bacteria will reside in the surface soil, and may transfer to plant surfaces where they may be taken up by the plant. This project aims to address the knowledge gap on how ARGs are distributed in soil following the land application of fertilizer, and whether bacteria carrying ARGs survive in and on plants. This research can help determine the significance of plants as a vector for the transport of ARGs to humans. This knowledge can guide the development of management practices to prevent or minimize the uptake of ARGs to edible plants, thus protecting the Nation's food supply for the benefit of public health.The goal for this project is to improve our understanding of the mechanisms that govern the fate and transport of antibiotic resistance genes (ARGs) in soil-plant ecosystems under field conditions. Three research objectives are established for the project: 1) characterize genetic resistance genes (resistome) in surface and rhizosphere soils following manure application and determine the correlation of the resistome with root exudates and antibiotics; 2) identify the source and measure the diversity of the resistome in the endophytic and epiphytic microbial communities in and on plants; 3) elucidate the metabolic adaptations that ARG-carrying bacteria use to survive in and on plants. Shotgun metagenomics will be used to study the resistome of the microbial communities in soil and plant samples, and quantitative proteomics will be used to study the protein expression of ARG-carrying bacteria living on and in plants. The research team will disseminate research findings directly to farmers and crop producers through agricultural extension education materials. The project education plan will focus on enriching student learning experiences with interdisciplinary knowledge of engineering, agriculture, and environmental science. Undergraduate students from underrepresented groups will be recruited to the project, and a new course module will be developed to show students the behaviors of ARGs in the environment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

抗生素耐药基因(ARGs)使细菌对抗生素产生耐药性，而携带ARGs的致病病原菌可使抗生素治疗感染性死亡的效果降低，从而威胁公众健康。土壤是ARG的主要环境储存库，在含有ARG的土壤中种植的可食用作物有可能成为以ARG为食的人和牲畜的ARG载体。一些携带ARG的细菌被引入农田土壤后，会停留在表层土壤中，并可能转移到植物表面，在那里它们可能被植物吸收。该项目旨在解决在土地施肥后ARGs如何在土壤中分布的知识差距，以及携带ARGs的细菌是否在植物体内和植物身上存活。这项研究有助于确定植物作为向人类运送ARG的媒介的重要性。这些知识可以指导管理实践的发展，以防止或最大限度地减少ARGs对可食用植物的吸收，从而保护国家的食品供应，以造福公众健康。该项目的目标是提高我们对田间条件下土壤-植物生态系统中抗生素抗性基因(ARGs)命运和运输机制的理解。该项目确定了三个研究目标：1)表征施用有机肥后表层和根际土壤中的遗传抗性基因(抗性组)，并确定抗性组与根分泌物和抗生素的相关性；2)鉴定植物内生和附生微生物群落中抗性组的来源并测量其多样性；3)阐明携带ARG的细菌在植物体内和植物上生存所使用的代谢适应。鸟枪式元基因组学将用于研究土壤和植物样品中微生物群落的抗药性，定量蛋白质组学将用于研究生活在植物和植物中的携带精氨酸的细菌的蛋白质表达。研究小组将通过农业推广教育材料直接向农民和作物生产者传播研究成果。该项目教育计划将侧重于通过工程、农业和环境科学的跨学科知识来丰富学生的学习经验。来自代表性不足群体的本科生将被招募到该项目中，并将开发一个新的课程模块，向学生展示ARGs在环境中的行为。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Predicting antibiotic resistance gene abundance in activated sludge using shotgun metagenomics and machine learning

使用鸟枪宏基因组学和机器学习预测活性污泥中抗生素抗性基因的丰度

• DOI: 10.1016/j.watres.2021.117384
• 发表时间: 2021
• 期刊: Water Research
• 影响因子: 12.8
• 作者: Sun, Yuepeng;Clarke, Bertrand;Clarke, Jennifer;Li, Xu
• 通讯作者: Li, Xu

Biotransformation of doxycycline by Brevundimonas naejangsanensis and Sphingobacterium mizutaii strains

• DOI: 10.1016/j.jhazmat.2021.125126
• 发表时间: 2021-01-22
• 期刊: JOURNAL OF HAZARDOUS MATERIALS
• 影响因子: 13.6
• 作者: He, Ting;Bao, Jianguo;Li, Xu
• 通讯作者: Li, Xu

Effects of Nutrient Level and Growth Rate on the Conjugation Process That Transfers Mobile Antibiotic Resistance Genes in Continuous Cultures

• DOI: 10.1128/aem.01121-22
• 发表时间: 2022-09
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: Mohammadreza Shafieifini;Yuepeng Sun;Zachery R. Staley;Jean‐Jack Riethoven;Xu Li