SBIR Phase II: High-Power and High-Energy-Density Enzymatic Fuel Cell through an In Vitro Synthetic Enzymatic Pathway

SBIR 第二阶段：通过体外合成酶途径的高功率和高能量密度酶燃料电池

• 批准号: 1353266
• 负责人: Zhiguang Zhu
• 金额: $ 75万
• 依托单位: Cell-Free Bioinnovations Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353266&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Power; Energy

This Small Business Innovation Research Phase II project seeks the further development of metal-free enzymatic fuel cells (EFCs) that can completely oxidize sugar (maltodextrin) to yield electricity. Sugar-powered EFCs have received increasing interest as next-generation, environmentally friendly bio-batteries. These bio-batteries, which will be fully biodegradable, non toxic, and easily refillable, are envisioned as next-generation green power sources, particularly for portable electronic devices (e.g., smartphones, portable computers, tablets, and GPS systems to name a few). These Sugar-powered EFCs with a feed of 20% maltodextrin utilizing a proprietary synthetic cocktail of enzyme catalysts for sugar oxidation, have an energy storage density of approximately 800 Ah/kg, nearly 20-times that of lithium batteries. The technical objectives of this project are the following: (i) to further increase their power density to 10 mW/cm2, (ii) to prolong their lifetimes to months, and (iii) to develop a commercial cost competitive product. The use of a low-cost chemical ingredient along with the use of engineered redox enzymes in sugar biobatteries, could increase their power output, prolong their lifetime, and decrease their production cost. In this SBIR II project, Cell Free Bioinnovations Inc. will make prototype high energy density power banks that could charge smartphones directly by integrating synthetic biology, protein engineering, nanobiotechnology, and electrochemistry.The broader impact/commercial potential of this project is the demonstration of the prototype bio-inspired sugar-powered biobatteries featuring high-energy storage density (e.g., 800 Ah electricity/kg of 20% sugar solution, nearly twenty times that of lithium ion batteries), fast refilling by the addition of a sugar solution, non toxic and safe to handle and use. Because sugar-powered EFCs are based on low-cost biocatalysts and do not require costly or rare metals, they are disposable and biodegradable devices. High-energy storage density EFCs would have broad potential applications as rechargeable battery chargers (e.g., cellular phone chargers for outdoor use or for portable military devices), educational toy kits, and disposable (primary) batteries. In the future, sugar-powered EFCs could potentially replace some secondary (rechargeable) batteries and primary batteries. In addition, the innovation of EFCs equipped with this cell-free synthetic pathway would greatly promote the concept of cell-free biomanufacturing composed of synthetic enzymatic pathways.

这个小企业创新研究第二阶段项目寻求进一步开发无金属酶燃料电池（EFC），该电池可以完全氧化糖（麦芽糖糊精）来发电。以糖为动力的 EFC 作为下一代环保生物电池越来越受到人们的关注。这些生物电池完全可生物降解、无毒且易于充电，被视为下一代绿色电源，特别是便携式电子设备（例如智能手机、便携式计算机、平板电脑和 GPS 系统等）。这些以糖为动力的 EFC 以 20% 麦芽糖糊精为原料，利用专有的酶催化剂合成混合物进行糖氧化，其能量存储密度约为 800 Ah/kg，几乎是锂电池的 20 倍。该项目的技术目标如下：(i) 将其功率密度进一步提高至 10 mW/cm2，(ii) 将其使用寿命延长至数月，以及 (iii) 开发具有商业成本竞争力的产品。在糖生物电池中使用低成本化学成分以及工程氧化还原酶可以增加其功率输出，延长其使用寿命并降低其生产成本。在这个 SBIR II 项目中，Cell Free Bioinnovations Inc. 将通过集成合成生物学、蛋白质工程、纳米生物技术和电化学来制造原型高能量密度移动电源，可以直接为智能手机充电。该项目更广泛的影响/商业潜力是展示具有高能量存储密度（例如 800 Ah）的仿生糖动力生物电池原型 电量/kg 20%糖液，近20倍于锂离子电池），通过添加糖液快速补充，无毒，操作使用安全。由于糖动力 EFC 基于低成本生物催化剂，不需要昂贵或稀有金属，因此它们是一次性且可生物降解的设备。高能量存储密度 EFC 将具有广泛的潜在应用，如可充电电池充电器（例如，户外使用或便携式军事设备的手机充电器）、教育玩具套件和一次性（一次）电池。未来，糖动力 EFC 可能会取代一些二次（可充电）电池和一次电池。此外，配备这种无细胞合成途径的EFC的创新将极大地促进由合成酶途径组成的无细胞生物制造的概念。