IDBR: TYPE A: Highly Efficient and Rapid Viral Aerosol Detection System (HERVADS)

IDBR：A 型：高效快速病毒气溶胶检测系统 (HERVADS)

• 批准号: 1353423
• 负责人: Chang-Yu Wu
• 金额: $ 38.25万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353423&HistoricalAwards=false
• 关键词: IDBR; TYPE; Highly; Efficient; Rapid

An award is made to the University of Florida to develop a bioaerosol instrument whose purpose is to enable highly efficient and rapid detection of airborne viruses. Existing bioaerosol sampling systems are designed for bacterial aerosol collection/detection, which are very ineffective for capturing viral aerosols because viruses are much smaller than bacteria. In addition, conventional detection methods for viruses are time consuming and therefore do not allow determination in field investigation. The novel Highly Efficient and Rapid Viral Aerosol Detection System (HERVADS) utilizes cooling and mixing methods to enable particle size amplification so that the nanosized viruses can be efficiently collected from air (90% of the new device compared to 5-10% of the existing device). Integrated with a paper-based microfluidic device with selective and sensitive immunoassay, the instrument allows detection of sampled viruses in minutes, orders of magnitude faster than the current method (in days), thus enabling its uses in the field. Incorporation of electrostatic mechanism into the system further enables detection of viral aerosols even in low concentration. By working closely with an industrial partner, the research team will have frequent and in-time feedbacks that allow incorporation of critical features for successful commercial development in the future. The University of Florida?s Office of Technology Licensing will also assist the research team in feasibility assessment, dissemination and outreach. Successful development and implementation of the novel HERVADS will have significant societal impacts. The HERVADS will impact environmental field studies, enabling the sampling of viruses of environmental and agricultural importance and furthering our basic understanding of the natural diversity of viruses associated with a variety of environmental niches. The HERVADS can also be used to help monitor air quality in critical environments such as emergency rooms, detecting biological pathogen threats, and would be useful for monitoring respiratory pathogens in mass gatherings. In addition, the cohesive collaboration among mechanical engineering, environmental science and virology will train graduate students with multi- and inter-disciplinary knowledge. Involvement of undergraduate researchers will motivate and better prepare them for pursuit of advanced education. YouTube videos created for this project will be a vehicle to reach the public, for course materials, as well as to promote the interest in utilizing the HERVADS.This award is being made jointly by two Programs- (1) Instrument Development for Biological Research, in the Division of Biological Infrastructure (Biological Sciences Directorate), and (2) the Division of Chemical, Bioengineering, Environmental and Transport Systems (Engineering Directorate).

佛罗里达大学被授予一项开发生物气溶胶仪器的奖项，其目的是实现对空气传播病毒的高效和快速检测。现有的生物气溶胶采样系统是为收集/检测细菌气溶胶而设计的，这对捕获病毒气溶胶非常无效，因为病毒比细菌小得多。此外，传统的病毒检测方法非常耗时，因此不能在现场调查中进行确定。新型高效快速病毒气溶胶检测系统(HERVADS)利用冷却和混合方法实现颗粒尺寸放大，从而可以有效地从空气中收集纳米尺寸的病毒(新设备的90%，而现有设备的5%-10%)。该仪器与具有选择性和灵敏免疫分析的纸基微流控设备集成在一起，可以在几分钟内检测到采样的病毒，比目前的方法(以天为单位)快几个数量级，从而使其能够在现场使用。将静电机制结合到系统中，即使在低浓度下也能检测到病毒气溶胶。通过与行业合作伙伴的密切合作，研究团队将获得频繁和及时的反馈，使其能够纳入未来成功商业开发的关键功能。佛罗里达大学S技术许可办公室也将协助研究团队进行可行性评估、传播和推广。新型HERVADS的成功开发和实施将产生重大的社会影响。HERVADS将影响环境实地研究，使对环境和农业具有重要意义的病毒采样成为可能，并加深我们对与各种环境生态位相关的病毒自然多样性的基本了解。HERVADS还可用于帮助监测急诊室等关键环境的空气质量，检测生物病原体威胁，并将有助于监测大规模集会中的呼吸道病原体。此外，机械工程、环境科学和病毒学之间的紧密合作将培养具有多学科和跨学科知识的研究生。本科生研究人员的参与将激励他们，并为他们追求高等教育做好更好的准备。为这个项目制作的YouTube视频将成为一种接触公众的工具，提供课程材料，并促进人们对使用HERVADS的兴趣。该奖项由两个项目联合颁发-(1)生物基础设施司(生物科学局)的生物研究仪器开发，(2)化学、生物工程、环境和运输系统司(工程局)。