Optimization of Calcium and RNA multiplexed activity imaging for highly parallelized evaluation of cell type functions in deep-brain structures

钙和 RNA 多重活性成像的优化,用于高度并行评估深部脑结构中的细胞类型功能

基本信息

  • 批准号:
    10401603
  • 负责人:
  • 金额:
    $ 84.28万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-09-01 至 2026-07-31
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY A central goal of neuroscience is to understand animal behavior in the context of the information processing properties of neuron ensembles. Neurons are circuit nodes in the brain information processing network, but they are also cells that express hundreds of genes that may make important contributions to determining their activity patterns. The relationship between gene expression, neuron projections, neuron activity, and behavior have been, individually, major avenues for investigation in both molecular and systems neuroscience. Integrating these levels of investigation would greatly advance understanding the influence of molecular properties on neuron information processing characteristics in healthy brains and disease states. In this project, which we refer to as CaRMA 2.0, we propose to develop and democratize methodologies to integrate measurements of transcriptomics, projectomics, calcium dynamics, and behavior in deep-brain structures. This proposal builds on our past success to develop and implement Calcium and RNA Multiplexed Activity (CaRMA) imaging, which combines these levels of investigation into a single large-scale experiment. This is achieved by deep-brain two-photon calcium imaging in a behaving mouse, followed by removing and sectioning the brain, aligning the ex vivo image volume with the in vivo-imaged neurons, and then performing multiple rounds of RNA-fluorescence in situ hybridization (FISH) on the same tissue. To reliably produce these high dimensional, multi-modal datasets requires significant advancements for “Optimization of Transformative Technologies for Large Scale Recording and Modulation in the Nervous System” as indicated in this RFA-NS-18-019 program announcement. We will develop improved technologies for deep-brain calcium imaging as well as highly multiplexed gene expression analysis on the same cells and apply this pipeline to investigate the mPFC, hindbrain, and the role of the lateral hypothalamus in hunger, thirst, and fear.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Meng Cui其他文献

Meng Cui的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Meng Cui', 18)}}的其他基金

Optical gearbox for high speed neural recording
用于高速神经记录的光学齿轮箱
  • 批准号:
    10157026
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
Optical gearbox for high speed neural recording
用于高速神经记录的光学齿轮箱
  • 批准号:
    10385852
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
Optimization of Clear Optically Matched Panoramic Access Channel Technique (COMPACT) for large-scale deep-brain neurophotonic interface
大规模深脑神经光子接口的清晰光学匹配全景访问通道技术(COMPACT)的优化
  • 批准号:
    10267684
  • 财政年份:
    2020
  • 资助金额:
    $ 84.28万
  • 项目类别:
Super-resolution deep tissue imaging of dendritic spines
树突棘的超分辨率深层组织成像
  • 批准号:
    9269018
  • 财政年份:
    2015
  • 资助金额:
    $ 84.28万
  • 项目类别:

相似海外基金

Wireless CMOS device for observing real-time brain activity and animal behavior
用于观察实时大脑活动和动物行为的无线 CMOS 设备
  • 批准号:
    23K06786
  • 财政年份:
    2023
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Animal, Behavior and Tissue Core
动物、行为和组织核心
  • 批准号:
    10496282
  • 财政年份:
    2023
  • 资助金额:
    $ 84.28万
  • 项目类别:
Landscapes of fear in the Anthropocene: Linking predation risk and human disturbance to animal behavior and ecological outcomes
人类世的恐惧景观:将捕食风险和人类干扰与动物行为和生态结果联系起来
  • 批准号:
    RGPIN-2022-03096
  • 财政年份:
    2022
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Discovery Grants Program - Individual
The role of biological interactions in the evolution of animal behavior
生物相互作用在动物行为进化中的作用
  • 批准号:
    RGPIN-2019-06689
  • 财政年份:
    2022
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Discovery Grants Program - Individual
Development of Semi-Supervised Learning Method using Compressed Video for Real-Time Animal Behavior Analysis
使用压缩视频进行实时动物行为分析的半监督学习方法的开发
  • 批准号:
    22H03637
  • 财政年份:
    2022
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Landscapes of fear in the Anthropocene: Linking predation risk and human disturbance to animal behavior and ecological outcomes
人类世的恐惧景观:将捕食风险和人类干扰与动物行为和生态结果联系起来
  • 批准号:
    DGECR-2022-00323
  • 财政年份:
    2022
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Discovery Launch Supplement
Neural and molecular mechanisms of microbe-sensing in the control of animal behavior - Resubmission - 1
微生物传感控制动物行为的神经和分子机制 - 重新提交 - 1
  • 批准号:
    10315486
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
REU Site: Animal Behavior in Context
REU 网站:背景下的动物行为
  • 批准号:
    2050311
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
    Standard Grant
Neural and molecular mechanisms of microbe-sensing in the control of animal behavior - Resubmission - 1
微生物传感控制动物行为的神经和分子机制 - 重新提交 - 1
  • 批准号:
    10412977
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
Molecular recording to predict cell fate decisions and animal behavior
分子记录预测细胞命运决定和动物行为
  • 批准号:
    10260139
  • 财政年份:
    2021
  • 资助金额:
    $ 84.28万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了