Autophagy and ER stress in plants

植物自噬和内质网应激

• 批准号: 1353867
• 负责人: Diane Bassham
• 金额: $ 43.78万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-15 至 2017-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353867&HistoricalAwards=false
• 关键词: Autophagy; ER; stress; plants

Diane C BasshamProposal number IOS- 1353867, Autophagy and ER stress in plants.Tolerance of changing environmental conditions is critical for plant growth and survival as they are unable to seek refuge from the environment. A key unresolved issue is how plants adapt their cellular functions to respond to stress conditions such as heat and drought. Two pathways required for stress tolerance are autophagy, in which damaged cell components are broken down and recycled, and the unfolded protein response, in which misfolded proteins activate the expression of genes that mitigate stress damage. In this project, the relationship between these two pathways will be examined. Additional proteins that are required for the regulation of both of these pathways will be identified and their functions analyzed by a combination of biochemical, cell biology and genetic approaches. The mechanism by which cells select substrates for recycling by autophagy will also be determined. This will provide insight into how plants sense adverse environmental conditions, how stress response pathways are then activated and how different responses work together to produce stress tolerance. The results of this project could provide information leading to the production of stress tolerant varieties of agriculturally important plants. The project will provide research training in plant genetics, cell and molecular biology for graduate and undergraduate students. In addition, the results of the research will be incorporated into a module of the Meta!Blast educational video game to help undergraduate and high school students learn cell biology. Seed stocks and plasmids generated in the course of the research will be donated to the Arabidopsis Biological Resource Center for distribution to the scientific community.

Diane C Bassham建议编号IOS- 1353867，植物中的自噬和内质网应激。植物对环境条件变化的耐受性对于植物生长和存活至关重要，因为它们无法从环境中寻求庇护。一个尚未解决的关键问题是植物如何调整其细胞功能以应对高温和干旱等胁迫条件。胁迫耐受所需的两种途径是自噬，其中受损的细胞成分被分解和回收，以及未折叠的蛋白质反应，其中错误折叠的蛋白质激活减轻胁迫损伤的基因的表达。在这个项目中，这两个途径之间的关系将被检查。这两种途径的调节所需的其他蛋白质将被确定，并通过生物化学，细胞生物学和遗传学方法的组合分析其功能。细胞通过自噬选择底物进行再循环的机制也将被确定。这将提供深入了解植物如何感知不利的环境条件，如何激活应激反应途径，以及不同的反应如何共同作用以产生胁迫耐受性。该项目的结果可以提供信息，导致农业上重要植物的耐胁迫品种的生产。该项目将为研究生和本科生提供植物遗传学、细胞和分子生物学方面的研究培训。此外，研究结果将被纳入一个模块的Meta！爆炸教育视频游戏，以帮助本科生和高中生学习细胞生物学。研究过程中产生的种子和质粒将捐赠给拟南芥生物资源中心，以分发给科学界。