REGULATORY NETWORKS THAT CONTROL AUTOPHAGY TO ENABLE ENVIRONMENTAL STRESS RESILIENCE IN PLANTS

控制自噬以实现植物环境应激恢复的调控网络

• 批准号: 2040582
• 负责人: Diane Bassham
• 金额: $ 79.6万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2040582&HistoricalAwards=false
• 关键词: REGULATORY; NETWORKS; CONTROL; AUTOPHAGY; ENABLE

This research will identify cellular mechanisms by which plants respond to and survive adverse environmental conditions; these mechanisms are key to their survival and productivity. Plants frequently encounter environmental stresses, such as drought, heat and flooding, both in their natural environment and in the field in agricultural settings. A major pathway for cell survival during stress is called autophagy, in which damaged cell components are digested and recycled, preventing their toxic accumulation. Substantial information about the autophagy process itself is available, but how autophagy is activated by environmental stress in plants is not yet clear. This research will address this problem by identifying factors that control autophagy and determining their function in stress tolerance. This potentially will lead to new approaches to improve stress tolerance in crop plants, thus enhancing their growth and yield. The project will train a postdoctoral scientist, a graduate student, and undergraduate students in research skills and scientific communication. It will also increase the accessibility of the cell biology scientific literature to undergraduates by annotation of research papers, which can be used in cell biology classes and will be shared broadly online with the scientific community. The effectiveness of this annotation will be assessed using validated pre- and post-intervention surveys to measure student increases in self-efficacy, competence and motivational beliefs.The activation of autophagy is a key component of plant responses to environmental stress, and is thought to be regulated via complex pathways that are coordinated with other stress responses to optimize plant growth and survival. Despite this critical role, very few regulatory factors for autophagy have yet been identified and characterized in plants. The goal of this project is to identify previously undescribed pathways for the regulation of autophagy in plants that act both transcriptionally and post-translationally. We will assess the mechanisms by which phosphorylation cascades involving the protein kinase SnRK1 control autophagy under different conditions, and determine the function of a suite of transcription factors that control autophagy gene expression, some of which are potentially regulated by SnRK1. This will be accomplished by genetic approaches to assess function in autophagy and epistasis among factors under different stress conditions, mutation of potential phosphorylation sites, phosphoproteomic analysis, and finally the construction of regulatory networks that integrate both phosphorylation and transcriptional activation events. The regulatory network analysis will in turn allow us to begin addressing how these pathways integrate and cooperate with other stress signaling pathways to enable plant resilience and survival in the face of adverse and changing conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项研究将确定植物对不利环境条件作出反应并生存下来的细胞机制;这些机制是它们生存和生产力的关键。植物在其自然环境中和在农业环境中的田间都经常遇到环境胁迫，例如干旱、高温和洪水。细胞在压力下存活的一个主要途径被称为自噬，其中受损的细胞成分被消化和回收，防止它们的毒性积累。关于自噬过程本身的大量信息是可用的，但植物中自噬如何被环境胁迫激活尚不清楚。这项研究将通过确定控制自噬的因素并确定它们在应激耐受中的功能来解决这个问题。这可能会导致新的方法来提高作物的抗逆性，从而提高其生长和产量。该项目将在研究技能和科学交流方面培训一名博士后科学家、一名研究生和一名本科生。它还将通过研究论文的注释增加本科生对细胞生物学科学文献的可访问性，这些论文可用于细胞生物学课程，并将与科学界广泛在线共享。 这种注释的有效性将通过有效的干预前和干预后调查来评估，以衡量学生自我效能，能力和动机信念的增加。自噬的激活是植物对环境胁迫反应的关键组成部分，并被认为是通过与其他胁迫反应协调的复杂途径来调节，以优化植物生长和生存。尽管有这种关键作用，但在植物中很少有自噬的调节因子被鉴定和表征。该项目的目标是确定以前未描述的调节植物自噬的途径，这些途径在转录和转录后都起作用。我们将评估涉及蛋白激酶SnRK 1的磷酸化级联反应在不同条件下控制自噬的机制，并确定一套控制自噬基因表达的转录因子的功能，其中一些可能受SnRK 1调控。这将通过遗传学方法来评估不同应激条件下因子之间自噬和上位性的功能，潜在磷酸化位点的突变，磷酸化蛋白质组学分析，以及最后整合磷酸化和转录激活事件的调控网络的构建来实现。调控网络分析反过来将使我们开始解决这些途径如何整合和合作与其他压力信号途径，使植物的弹性和生存在面对不利和不断变化的条件。这个奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

A positive feedback regulation of SnRK1 signaling by autophagy in plants

植物中自噬对 SnRK1 信号的正反馈调节

• DOI: 10.1016/j.molp.2023.07.001
• 发表时间: 2023
• 期刊: Molecular Plant
• 影响因子: 27.5
• 作者: Yang, Chao;Li, Xibao;Yang, Lianming;Chen, Shunquan;Liao, Jun;Li, Kailin;Zhou, Jun;Shen, Wenjin;Zhuang, Xiaohong;Bai, Mingyi
• 通讯作者: Bai, Mingyi

PROTAC for agriculture: learning from human medicine to generate new biotechnological weed control solutions

PROTAC 用于农业：学习人类医学以产生新的生物技术杂草控制解决方案

• DOI: 10.1002/ps.7741
• 发表时间: 2023
• 期刊: Pest Management Science
• 影响因子: 4.1
• 作者: Leon, Ramon G.;Bassham, Diane C.
• 通讯作者: Bassham, Diane C.

Studying plant autophagy: challenges and recommended methodologies

• DOI: 10.1007/s44307-023-00002-8
• 发表时间: 2023-10
• 期刊: Advanced Biotechnology
• 作者: Hua Qi;Yao Wang;Yan Bao;D. Bassham;Liang Chen;Qin-Fang Chen;Suiwen Hou;Inhwan Hwang