CYTOSKELETON FUNCTION AND DYNAMICS DURING PLANT VACUOLAR AUTOPHAGY IN RESPONSE TO ENVIRONMENTAL STRESSES

植物液泡自噬响应环境胁迫期间的细胞骨架功能和动力学

• 批准号: 0515998
• 负责人: Diane Bassham
• 金额: $ 37.5万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0515998&HistoricalAwards=false
• 关键词: CYTOSKELETON; FUNCTION; DYNAMICS; DURING; PLANT

Autophagy (meaning 'self-eating') is a process that takes place in animal, plant and fungal cells during starvation and senescence (aging). During this process, cellular components are broken down into smaller molecules as a means to recycle nutrients for re-use by the organism. This degradation takes place inside a cellular compartment called the vacuole. The aim of the proposed research is to determine how components are delivered to the vacuole for degradation during nutrient starvation in the model plant Arabidopsis thaliana. The mechanism of delivery will be characterized using microscopy together with chemicals that prevent transport. In addition, the function of a gene that is likely to be involved in the transport process will be analyzed using genetic and biochemical approaches. This research is expected to provide insight into the importance of autophagy in the response of plants to nutrient starvation and to elucidate the mechanism of transport of components to the vacuole during autophagy. This will add to our knowledge of a fundamental process that is important in stress responses and disease progression in many different organisms. It also has agricultural implications, as nutrient deficiency is often encountered by crop plants in the field, and a greater understanding of the response of plants to these conditions may lead to new strategies to combat the deficiency. An integral part of this proposal is the training of graduate and undergraduate students in research. This project will provide opportunities for students to gain laboratory experience through a number of different programs, including programs designed to increase the participation of women and minority students in scientific research.

自噬（意为“自我吞噬”）是动物、植物和真菌细胞在饥饿和衰老过程中发生的过程。在此过程中，细胞成分被分解成更小的分子，作为回收营养物质供生物体再利用的一种手段。这种降解发生在称为液泡的细胞室内。拟议研究的目的是确定在模式植物拟南芥营养饥饿期间如何将成分传递到液泡进行降解。将使用显微镜和阻止运输的化学物质来表征递送机制。此外，将使用遗传和生化方法分析可能参与运输过程的基因的功能。这项研究有望深入了解自噬在植物应对营养饥饿中的重要性，并阐明自噬过程中成分运输至液泡的机制。这将增加我们对许多不同生物体的应激反应和疾病进展中重要的基本过程的了解。它还具有农业意义，因为田间作物经常遇到养分缺乏的情况，更好地了解植物对这些条件的反应可能会导致解决养分缺乏的新策略。该提案的一个组成部分是研究生和本科生的研究培训。该项目将为学生提供通过许多不同项目获得实验室经验的机会，包括旨在增加女性和少数民族学生参与科学研究的项目。