IDBR: Type A - Improving 3D resolution and reducing sensitivity to spherical aberration in live, thick sample cellular imaging using novel methods in optical sectioning microscopy

IDBR：A 型 - 使用光学切片显微镜中的新方法提高活体厚样品细胞成像中的 3D 分辨率并降低对球面像差的敏感性

• 批准号: 1353904
• 负责人: Chrysanthe Preza
• 金额: $ 74.45万
• 依托单位: University of Memphis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353904&HistoricalAwards=false
• 关键词: IDBR; Type; Improving; 3D; resolution

This award is being made to the University of Memphis by the Instrumentation Development for Biological Research program in the Directorate for Biological Sciences.Several areas of biological and biomedical research critically depend on 3D imaging for accurate analysis of subcellular structures within thick cellular samples. This research project will create a new imaging system design based on integrated computational and optical techniques to overcome the depth limitations of high-resolution 3D microscope imaging. The collaborative nature of the research is a model for cooperation between basic scientists and engineers. Outreach activities associated with this project will provide opportunities for local undergraduate students and community college instructors to participate in this research, connect with biological researchers and the international microscopy community, and gain skills in modern microscopy techniques.This project will develop a computational optical system for three-dimensional (3D) microscopy imaging. The research involves integrated structured illumination optics, phase modulated imaging optics, digital signal processing, and analysis/synthesis techniques to achieve results not obtainable from traditional imaging standards. A novel partially coherent illumination configuration will be designed and tested and an imaging system uniquely capable of insensitivity to sample-induced aberration will be engineered. Digital processing algorithms that result in high-resolution, low-noise 3D images at depths of up to 100 micrometers within a thick sample will be developed. The broader impacts of this work include enabling of new capabilities developed to address currently inaccessible biological questions as well as potential societal benefits from implementation of a model for interdisciplinary education that encourages young scientists and engineers to engage each other in the drive toward innovation and discovery. Dissemination plans include a) a 3-day workshop/training session for potential users and industry partners, and b) online plans and instructions to build the system and the right to non-profit institutions to use it for research.

该奖项由生物科学理事会的生物研究仪器开发项目授予孟菲斯大学。生物和生物医学研究的多个领域严重依赖 3D 成像来准确分析厚细胞样本中的亚细胞结构。该研究项目将创建一种基于集成计算和光学技术的新成像系统设计，以克服高分辨率 3D 显微镜成像的深度限制。研究的协作性质是基础科学家和工程师之间合作的典范。与该项目相关的外展活动将为当地本科生和社区学院教师提供参与这项研究的机会，与生物研究人员和国际显微镜界建立联系，并获得现代显微镜技术的技能。该项目将开发用于三维（3D）显微镜成像的计算光学系统。该研究涉及集成结构照明光学器件、相位调制成像光学器件、数字信号处理和分析/合成技术，以实现传统成像标准无法获得的结果。将设计和测试一种新颖的部分相干照明配置，并且将设计一种独特的能够对样本引起的像差不敏感的成像系统。我们将开发数字处理算法，在厚样品中生成深度达 100 微米的高分辨率、低噪声 3D 图像。这项工作的更广泛影响包括开发新的能力来解决目前无法解决的生物学问题，以及实施跨学科教育模型的潜在社会效益，该模型鼓励年轻科学家和工程师相互参与，共同推动创新和发现。传播计划包括 a) 为潜在用户和行业合作伙伴举办为期 3 天的研讨会/培训课程，以及 b) 构建系统的在线计划和说明以及非营利机构使用该系统进行研究的权利。