Collaborative Research: Strain in Cartilaginous Fish Skeletons

骨应变的直接分析为功能形态学家提供了一种测量各种行为表现的技术。然而，由于无法使用应变计或类似工具，骨骼软骨的测量一直受到阻碍。本研究项目将使用一种新颖的方法，声学测量法，来研究软骨脊椎动物作为结构材料的功能。初步研究表明，超声显微测量法可以成功地用于测量软骨的应变。该研究将通过量化体外和体内加载过程中的应变，提高我们对脊椎动物软骨作为结构材料的功能的认识和理解。该研究还将为其他生物材料的研究开辟多种途径，这些途径不适合传统的菌株分析。广泛的软骨鱼和软骨鱼在颌骨和舌骨结构的形态和运动学上都有所不同，将在进食和通气行为中进行研究，目的有五个。形态学变化将使用三维几何形态计量学进行量化。机械性能将在体外测量，以提供基线数据，以评估软骨作为结构材料的功能。活鱼的应变将被测量来检查软骨作为骨骼元件的性能，为测量非骨骨骼元件的应变提供技术和基线。有限元分析将在体内物种中进行验证，并用于预测那些物种或不适合体内技术的结构的应变。软骨的性能，行为和形态将分析系统发育揭示生态和进化模式。一名研究员，两名研究生和至少七名本科生将在研究的各个方面接受研究培训，从数据收集到发表。该项目还将包括K-12外展、科学教师培训和本科教育。研究过程中产生的有限元模型将存档在可公开访问的BIOMESH数据库（http://www.biomesh.org/）中。

Direct analysis of bone strain has provided functional morphologists with a technique to measure performance in a tremendous variety of behaviors. However, measurement in skeletal cartilage has been hampered by the inability to use strain gauges or similar tools. This research project will use a novel approach, sonomicrometry, to investigate the function of cartilage as a structural material in cartilaginous vertebrates. Preliminary research shows that sonomicrometry, which uses ultrasound to measure deformation, can be used successfully to measure strain in cartilage. The research will advance our knowledge and understanding about the function of cartilage as a structural material in vertebrates by quantifying strain during in vitro and in vivo loading. The study will also open multiple avenues of study on other biological materials that are not amenable to traditional strain analysis.A broad range of chondrichthyan and chondrostean fishes that vary morphologically and kinematically in jaw and hyoid structures will be studied during feeding and ventilation behaviors with five aims. Morphological variation will be quantified using 3D geometric morphometrics. Mechanical properties will be measured in vitro to provide baseline data to assess the function of cartilage as a structural material. Strain in living fishes will be measured to examine cartilage performance as a skeletal element, providing a technique and baseline for measuring strain in non-bony skeletal elements. Finite element analysis will be validated from in vivo species and used to predict strain in those species or structures that are not amenable to in vivo techniques. Cartilage performance, behavior, and morphology will be analyzed phylogenetically to uncover ecological and evolutionary patterns. A research fellow, two graduate students and at least seven undergraduate students will receive research training in all aspects of the study, from data collection to publication. The project will also include K-12 outreach, science teacher-training and undergraduate education. FE models produced during the study will be archived in the publically accessible BIOMESH database (http://www.biomesh.org/).