DISSERTATION RESEARCH: Divergence of long intergenic noncoding RNAs in Drosophila pseudoobscura, Drosophila persimilis, and their hybrids

论文研究：假暗果蝇、Persimilis 果蝇及其杂交种中长基因间非编码 RNA 的分化

• 批准号: 1405103
• 负责人: Carlos Machado
• 金额: $ 1.96万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1405103&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Divergence; long; intergenic

Long intergenic non-coding RNAs (lincRNAs) are a recently discovered group of transcripts that have no protein-coding capacity. Although they play diverse and important roles in the biology of complex organisms, almost nothing is known of their origin and evolution. The goal of this project is to use next generation RNA sequencing technology (RNA-Seq) and the vast genomic resources of the fruit fly Drosophila to characterize the forces that underlie lincRNA sequence and expression evolution. Analyses of variation in pure species cannot explain how the regulation of lincRNA expression evolves, but analyses of misexpression in hybrids provides insight into how diverse evolutionary processes shape the regulatory process. Thus, the specific goal of this dissertation improvement grant is to analyze the evolution of lincRNA regulation using RNA-Seq in D. pseudoobscura/D. persimilis hybrids. Specific aims are (1) to identify the mode of inheritance of lincRNA expression, (2) identify whether lincRNA regulatory divergence occurs through cis or trans changes, and (3) identify whether lincRNAs may underlie hybrid incompatibilities.This work will be the most comprehensive analysis of lincRNA evolution to date and the first to examine lincRNA regulatory evolution in closely related species via hybrids. It will underscore the importance of an understudied class of genes that deviates from the central dogma of biology. This grant will support the training of both a doctoral student and multiple undergraduates as well as provide a summer research experience for a local high school student or teacher from the University of Maryland's Minority Student Pipeline. The knowledge gleaned from this work will be disseminated widely, both in national presentations and in print. Understanding the evolution of gene regulatory systems provides important insights into the relationship between genotype and phenotype, which ultimately underlies our understanding of disease and the improvement of agricultural crops.

长长的基因间非编码RNA（LincrNA）是最近发现的一组没有蛋白质编码能力的转录本。尽管它们在复杂生物的生物学中扮演着多种和重要的作用，但几乎没有什么知识的起源和进化。该项目的目的是使用下一代RNA测序技术（RNA-Seq）和果蝇果蝇的庞大基因组资源来表征林克纳序列和表达演化的力的力。纯物种变异的分析无法解释lincrna表达的调节如何发展，但是杂种中Misexpression的分析提供了对各种进化过程如何影响调节过程的洞察力。因此，本论文改进的具体目标是使用D. pseudoobscura/d中的RNA-Seq分析Lincrna调节的演变。 Persimilis杂种。 Specific aims are (1) to identify the mode of inheritance of lincRNA expression, (2) identify whether lincRNA regulatory divergence occurs through cis or trans changes, and (3) identify whether lincRNAs may underlie hybrid incompatibilities.This work will be the most comprehensive analysis of lincRNA evolution to date and the first to examine lincRNA regulatory evolution in closely related species via hybrids.它将强调偏离生物学中心教条的一类研究基因的重要性。该赠款将支持对博士生和多个本科生的培训，并为马里兰大学少数族裔学生管道的当地高中生或老师提供夏季研究经验。从国家演讲和印刷中，这项工作中收集的知识将被广泛传播。了解基因调节系统的演变提供了对基因型和表型之间关系的重要见解，这最终奠定了我们对疾病的理解以及农作物的改善。