DISSERTATION RESEARCH: Investigating the impact of hybridization on diversification: A case study in the plant genus Heuchera

论文研究：调查杂交对多样化的影响：以 Heuchera 属植物为例

• 批准号: 1406721
• 负责人: John Freudenstein
• 金额: $ 1.55万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1406721&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Investigating; impact; hybridization

Hybridization is broadly relevant because it is the basis for much of the diversity around us, including agricultural crops and pests. The existence of successful ancient natural hybrids between species challenges traditional notions in biology that hybrids are ephemeral dead-ends in the evolution of biodiversity. However, detecting ancient hybrids using genetic data is challenging since hybrids have a "mosaic" of genes possessing different underlying histories and traits. In this project, the investigators will test hypotheses about ancient hybridizations, specifically whether Pleistocene glaciations have enabled hybridization by causing range shifts. The proposed work will be conducted within the genus Heuchera by comparing species richness between hybrid and parental lineages in several parallel cases that have been identified. This project analyzes Coral Bells (Heuchera) from a DNA and morphological perspective to investigate the long-term contribution of hybridization to evolution. The objectives of this study include (1) the discovery of hybrids, (2) examination of relative rates of species formation with parents, and (3) reconstruction of historical geographical ranges. This study contributes to scientific infrastructure through the provision of DNA sequences for comparative studies, data for the inference of evolutionary history, descriptions of new and rare species and varieties, and the creation of museum specimens and identification keys. The project includes training of undergraduates in research methods, the creation of pedagogical exercises for undergraduate classes, and public lectures on hybridization in general and on specific aspects of the project.

杂交具有广泛的相关性，因为它是我们周围许多多样性的基础，包括农作物和害虫。古代物种之间成功的自然杂交的存在挑战了生物学中的传统观念，即杂交是生物多样性进化中短暂的死胡同。然而，利用遗传数据检测古代杂交种具有挑战性，因为杂交种具有具有不同潜在历史和性状的基因“马赛克”。在这个项目中，研究人员将测试有关古代杂交的假设，特别是更新世冰川作用是否通过引起范围变化而实现了杂交。拟议的工作将在 Heuchera 属内进行，通过比较已确定的几个平行案例中杂种和亲本谱系之间的物种丰富度。该项目从 DNA 和形态学角度分析珊瑚钟 (Heuchera)，以研究杂交对进化的长期贡献。这项研究的目标包括（1）发现杂交种，（2）检查物种形成与亲本的相对速率，以及（3）重建历史地理范围。这项研究通过提供用于比较研究的 DNA 序列、用于推断进化历史的数据、新的和稀有物种和品种的描述以及博物馆标本和识别密钥的创建，为科学基础设施做出了贡献。该项目包括对本科生进行研究方法培训、为本科生课程创建教学练习以及关于杂交的一般性和项目具体方面的公开讲座。