Collaborative Research: Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments

合作研究：海洋酸化：微生物作为多种应激源适应性反应的哨兵：对比河口和公海环境

• 批准号: 1416673
• 负责人: Konstantinos Konstantinidis
• 金额: $ 36.64万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1416673&HistoricalAwards=false
• 关键词: Collaborative; Research; Ocean; Acidification; microbes

Collaborative Research: Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environmentsMicrobes in the oceans provide essential ecosystem services including primary production (photosynthesis) and organic matter turnover that sustain all higher marine organisms. It remains unclear how marine microorganisms will adapt to the acidification of the oceans (i.e., decrease in seawater pH due to increased atmospheric CO2) and how much their activities will be affected. It is also important to quantify how multiple stressors related to ocean acidification (OA) such as higher temperatures interact with increased acidity to represent the impact of multiple stressors on marine microbial communities. Advancing our understanding of these issues is essential in order to predict the consequences of OA on ocean life and fisheries, identify areas of the ocean that might be more vulnerable to OA, and propose modes of action to counteract potential adverse effects. This collaborative project by Duke University and Georgia Institute of Technology researchers will combine oceanographic and advanced molecular techniques to characterize the adaptive responses of microbial communities to multiple stressors associated with OA. In particular, microbial communities from estuarine and coastal ecosystems as well as open ocean waters will be incubated under conditions of increased acidity or temperature or both, and their activities will be measured and quantified. By integrating multiple types of data, this project will provide a predictive and mechanistic understanding of microbial community responses and feedbacks to OA, including essential ecosystem services. In addition to providing advanced training for undergraduate, graduate and postdoctoral students, results from this research project will be translated to broad public and K-12 education on OA through the development of an interactive museum display and iPAD application.Preliminary data from time-series observations of a coastal temperate estuary shows that pH, temperature and other stressors vary over multiple space and time scales, and this variability is relatively higher than that observed in open ocean waters. Based on this evidence, the guiding hypothesis of this work is that microbes in coastal ecosystems are better adapted to ocean acidification as well as multiple stressors compared to similar microbes from the open ocean. To quantify the adaptive genetic, physiological and biogeochemical responses of microbes to OA, the team's specific goals are to: (1) characterize complex natural microbial community responses to multiple stressors using factorial mesocosm manipulations, (2) assemble a detailed view of genomic and physiological (including transcriptional) adaptations to OA at the single species level using cultured model marine microbes (e.g. Prochlorococcus, Synechococcus, Vibrio) identified as responsive to stressors in whole community mesocosm experiments, and (3) assess the power of model microbial strains and mesocosm experiments to predict microbial community responses to natural OA variability in a temporally dynamic, temperate estuary and along a trophic/pH gradient from the Neuse-Pamlico Sound to the Sargasso Sea. By comparing an estuarine ecosystem to its open ocean counterpart, this study will assess the sensitivity of microbial structure and function in response to ocean acidification.

协作研究：海洋酸化：微生物作为对多种压力源的适应性反应的哨兵：海洋中的河口和开放海洋环境的对比提供了必不可少的生态系统服务，包括初级生产（光合作用）和有机物的离职和维持所有更高海洋生物的生态问题。 目前尚不清楚海洋微生物如何适应海洋的酸化（即由于大气中的二氧化碳增加而导致的海水pH值减少），以及它们的活性会受到多少影响。量化多种应激源与海洋酸化（OA）（例如较高温度）如何与酸度增加相互作用，以表示多种应激源对海洋微生物群落的影响，这也很重要。为了预测OA对海洋生物和渔业的后果，确定可能更容易受到OA的影响，并提出采取行动模式以抵消潜在的不利影响。杜克大学和佐治亚大学技术研究院研究人员的合作项目将结合海洋学和先进的分子技术，以表征微生物群落对与OA相关的多种压力源的适应性反应。 特别是，在酸度或温度升高或两者兼有的条件下，将孵育来自河口和沿海生态系统以及开放海水的微生物群落，并将测量和量化其活性。通过整合多种类型的数据，该项目将对微生物社区的反应和对OA的反馈（包括基本生态系统服务）提供预测性和机械理解。除了为本科，研究生和博士后学生提供高级培训外，该研究项目的结果还将通过开发互动博物馆的展示和iPad应用程序来转化为OA的广泛公共和K-12教育。从沿海温度的时间表观察到的pH，温度和其他范围的空间和时间范围相比，沿海温度的时间序列观察到了越来越高的空间和其他范围，以使其与该空间相关。基于这一证据，这项工作的指导假设是，与开阔的海洋类似的微生物相比，沿海生态系统中的微生物更好地适应了海洋酸化以及多种压力源。为了量化微生物对OA的适应性遗传，生理和生物地球化学反应，团队的具体目标是：（1）使用阶乘中型中的操纵来表征复杂的自然微生物社区对多种压力源的反应，（2）使用基因组和物理级别的criptation courtion（包括单个物理级别）（包括单个物种级别）（包括单个物理级别（包括单个物理）， Prochlorococcus, Synechococcus, Vibrio) identified as responsive to stressors in whole community mesocosm experiments, and (3) assess the power of model microbial strains and mesocosm experiments to predict microbial community responses to natural OA variability in a temporally dynamic, temperate estuary and along a trophic/pH gradient from the Neuse-Pamlico Sound to the Sargasso Sea.通过将河口生态系统与开阔海洋对应物进行比较，本研究将评估微生物结构和功能对海洋酸化的敏感性。

项目成果

Description of Candidatus Mesopelagibacter carboxydoxydans and Candidatus Anoxipelagibacter denitrificans: Nitrate-reducing SAR11 genera that dominate mesopelagic and anoxic marine zones

Candidatus Mesopelagibacter carboxydoxydans 和 Candidatus Anoxipelagibacter denitificans 的描述：主导中层和缺氧海洋区域的硝酸盐还原 SAR11 属

• DOI: 10.1016/j.syapm.2021.126185
• 发表时间: 2021
• 期刊: Systematic and Applied Microbiology
• 影响因子: 3.4
• 作者: Ruiz-Perez, Carlos A.;Bertagnolli, Anthony D.;Tsementzi, Despina;Woyke, Tanja;Stewart, Frank J.;Konstantinidis, Konstantinos T.
• 通讯作者: Konstantinidis, Konstantinos T.