MRI: Acquisition of a Zeiss Elyra Super Resolution Microscope for BRC Imaging facility at Cornell

MRI：为康奈尔大学 BRC 成像设施购买 Zeiss Elyra 超分辨率显微镜

• 批准号: 1428922
• 负责人: Warren Zipfel
• 金额: $ 78.99万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1428922&HistoricalAwards=false
• 关键词: MRI; Acquisition; Zeiss; Elyra; Super

An award is made to Cornell University to support the acquisition and technical management of a new type of light microscope that provides the resolution to see and differentiate features in living cells as small as 20 nm, or 5000 times smaller than the diameter of a human hair. This is more than a 10 fold improvement over what is achievable using a conventional light microscope. In addition to enabling new avenues of biological and biomedical research that are now impossible without it, the instrument will be located in the Cornell Imaging Core Facility so that it is open and available to investigators and students from all areas of scientific research inside and outside of Cornell. The imaging facility has a steady flow of students passing through it due to its participation in the numerous outreach programs occurring at Cornell. The facility hosts educational workshops and supports STEM initiatives on campus by providing tours and demonstration modules for a wide range of outreach activities. In particular, broadening the participation of women and under-represented minorities in engineering and biotechnology is a major focus at Cornell, and the facility routinely offers unique imaging laboratory modules for programs such as CATALYST and Expand Your Horizons. The acquisition of a unique and cutting edge super-resolution light microscope will provide graduate, undergraduate and local and visiting high school students with access to a type of instrument not available anywhere else in the region. It will make students of all ages aware of the remarkable developments in technology and engineering that are continually advancing fields such as imaging, and let them see the inner workings of living cells in ways that no one has ever before seen.The newly acquired microscope will be used in variety of research projects ranging from high resolution studies of the cellular biochemical signaling machinery, to investigations into the molecular structure of cellulose in research directed at improving biofuels. It will allow for the spatial, temporal and functional relationships between, for example, proteins that regulate transcription and the gene being transcribed to be mapped and quantified. The field of super-resolution microscopy is in its infancy and new and important applications of optical super-resolution are being demonstrated in all areas of the life sciences at a remarkable rate. Cornell has a high level of microscopy and biological imaging experience and a particularly broad repertoire of applications, and as such, is an ideal site for an instrument such as this to be located. The extensive microscopy expertise available at Cornell will lead to synergistic advances in biological imaging and extend the emerging field of super resolution microscopy through the development of new methodologies tailored for the specific applications of the investigators. This type of synergy will translate into the most significant and broadest possible impact of the research carried out using the instrument, and ultimately advance the knowledge base and understanding across diverse fields as the results are published and new technology broadly utilized.

康奈尔大学（Cornell University）获得了一项奖项，以支持获取和技术管理一种新型光学显微镜，该显微镜提供的分辨率可以观察和区分小至20纳米（比人类头发直径小5000倍）的活细胞的特征。这比使用传统光学显微镜所能达到的效果提高了10倍以上。除了为生物和生物医学研究提供新的途径，现在没有它是不可能的，该仪器将位于康奈尔成像核心设施，以便它对康奈尔大学内外所有科学研究领域的研究人员和学生开放和可用。由于参与了康奈尔大学的众多外展项目，成像设施有稳定的学生流量。该设施举办教育研讨会，并通过为广泛的外展活动提供参观和示范模块，支持校园内的STEM计划。特别是，扩大女性和少数族裔在工程和生物技术领域的参与是康奈尔大学的一个主要重点，该设施经常为CATALYST和Expand Your Horizons等项目提供独特的成像实验室模块。这台独特的、尖端的超分辨率光学显微镜将为研究生、本科生、当地和来访的高中生提供一种在该地区其他地方无法获得的仪器。它将使各个年龄段的学生都意识到技术和工程方面的显著发展，这些发展正在不断推动成像等领域的发展，并让他们以前所未有的方式看到活细胞的内部运作。新获得的显微镜将用于各种研究项目，从细胞生化信号机制的高分辨率研究，到研究纤维素的分子结构，以改善生物燃料的研究。它将允许空间、时间和功能之间的关系，例如，调节转录的蛋白质和被转录的基因之间的关系被绘制和量化。超分辨率显微技术尚处于起步阶段，光学超分辨率的重要新应用正以惊人的速度在生命科学的各个领域得到展示。康奈尔大学拥有高水平的显微镜和生物成像经验，以及特别广泛的应用程序，因此是放置此类仪器的理想场所。康奈尔大学广泛的显微镜专业知识将导致生物成像的协同进步，并通过为研究人员的特定应用量身定制的新方法的开发，扩展超分辨率显微镜的新兴领域。这种类型的协同作用将转化为使用该工具进行的研究的最重要和最广泛的可能影响，并最终随着结果的发表和新技术的广泛应用而推进知识库和跨不同领域的理解。