MRI: Development of Multiscale Imaging Platform for Quantitative Analysis of Collagen Organization

PI：Camagnola，Paul J.Proposal：1429045标题：MRI：开发用于胶原组织定量分析的多尺度成像平台意义该设备和实验平台将极大地扩展威斯康星州大学麦迪逊分校的研究基础设施，并为研究具有相关胶原蛋白改变的广泛疾病状态开辟新的可能性，这可能具有转换到各种临床应用的可能性。PI开发了光学显微镜研究生课程作为生物医学工程课程的一部分。PI和共同PI都在本科生/研究生调查课程“生命的显微镜”中讲授，该课程涵盖显微镜、生物医学成像技术和生物信息学。研究生将沉浸在一个高度跨学科的研究环境中，涉及癌症生物学、成像、光学工程和成像分析工程的各个方面。他们的培训还将提供接触不同可能的职业道路的机会，包括学术界和工业界。本科生和高中生也将参与到这个项目中来。这个合作项目将开发一个用于胶原组织定量分析的多尺度成像平台，通过几个MMS组织来询问~100 nm的尺寸尺度。该系统将采用增强型背向散射(EBS)技术，通过光学散射来表征纳米结构；激光扫描二次谐波(SHG)显微镜，用于亚微米和微米尺度的胶原蛋白可视化；以及定量超声(QUS)，用于量化组织结构的更大方面。与单独的相关测量相比，集成到单一平台提供了显著的优势。该仪器还将提供光学和声学散射的科学研究，并为未来的临床诊断能力提供框架。这一仪器平台的应用将集中在研究胶原及其在正常和疾病过程中的作用和变化。

PI: Campagnola, Paul J.Proposal: 1429045Title: MRI: Development of Multiscale Imaging Platform for Quantitative Analysis of Collagen OrganizationSignificanceThe equipment and experimental platform will greatly extend the UW-Madison research infrastructure and open up new possibilities for studying a wide range of disease states that have associated collagen alteration, which may have translational possibilities to a variety of clinical applications.The PI developed an optical microscopy graduate course as part of the biomedical engineering curriculum. The PI and co-PIs all lecture in the undergraduate/graduate survey course "the Microscopy of Life" which covers microscopy, biomedical imaging techniques, and bioinformatics. The graduate students will be immersed in a highly interdisciplinary research environment that involves aspects of cancer biology, imaging, optical engineering, and imaging analysis engineering. Their training will also provide exposure to different possible career paths including academia and industry. Undergraduates and high school students will also be involved in the project.Technical DescriptionThis collaborative project will develop a "Multiscale Imaging Platform for Quantitative Analysis of Collagen Organization" to interrogate size scales of ~100 nm through several mms of tissues. This system will incorporate Enhanced BackScattering (EBS) for characterization of nanoarchitecture by optical scattering, laser scanning Second Harmonic Generation (SHG) microscopy for collagen visualization at the submicron and micron scale, and Quantitative Ultrasound (QUS) for quantifying larger aspects of tissue organization. The integration into a single platform affords significant advantages over individual correlated measurements. The instrument will also afford both scientific studies of optical and acoustic scattering and also provide the framework for future clinical diagnostic capabilities. The applications of this instrumentation platform will be focused on investigating collagen and its role and alteration in normal and diseased processes.