SGER: Biophotonics: Nanoscale Fabrication of Tissue Engineering Scaffolds by Stimulated Emission Depletion Microscopy

SGER：生物光子学：通过受激发射损耗显微镜纳米级制造组织工程支架

• 批准号: 0630545
• 负责人: Paul Campagnola
• 金额: $ 15.94万
• 依托单位: University of Connecticut Health Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0630545&HistoricalAwards=false
• 关键词: SGER; Biophotonics; Nanoscale; Fabrication; Tissue

0630545CampagnolaThe applicant will implement the use of MPE Stimulated Emission Depletion (STED) microscopy as a nanofabrication tool in order to achieve minimum features sizes (50-100 nm) that are much smaller than possible through single beam MPE (~300-500 nm). The integration of STED with his current MPE fabrications methods will allow him to create tissue scaffolds with precisely defined micron and nanometer-sized features with the biochemical compositions that reproduce the native structure. Such replication is necessary to promote cellular functions such as adhesion, differentiation, proliferation, and Extracellular Matrix (ECM) synthesis and deposition.

申请人将使用MPE受激发射损耗（STED）显微镜作为纳米加工工具，以实现比单束MPE （~300-500 nm）小得多的最小特征尺寸（50-100 nm）。STED与他目前的MPE制造方法的整合将使他能够创建具有精确定义的微米和纳米尺寸特征的组织支架，其生化成分可以复制天然结构。这种复制是促进细胞功能如粘附、分化、增殖和细胞外基质（ECM）合成和沉积所必需的。