SGER: Biophotonics: Nanoscale Fabrication of Tissue Engineering Scaffolds by Stimulated Emission Depletion Microscopy

申请人将使用MPE受激发射耗尽(STED)显微镜作为纳米制造工具，以实现比单光束MPE(~300-500 nm)小得多的最小特征尺寸(50-100 nm)。将STED与他目前的MPE制造方法相结合，将使他能够创造具有精确定义的微米和纳米特征的组织支架，以及复制天然结构的生化成分。这种复制对于促进细胞功能，如黏附、分化、增殖以及细胞外基质(ECM)的合成和沉积是必要的。

0630545CampagnolaThe applicant will implement the use of MPE Stimulated Emission Depletion (STED) microscopy as a nanofabrication tool in order to achieve minimum features sizes (50-100 nm) that are much smaller than possible through single beam MPE (~300-500 nm). The integration of STED with his current MPE fabrications methods will allow him to create tissue scaffolds with precisely defined micron and nanometer-sized features with the biochemical compositions that reproduce the native structure. Such replication is necessary to promote cellular functions such as adhesion, differentiation, proliferation, and Extracellular Matrix (ECM) synthesis and deposition.