SBIR Phase II: Ultra Rapid Genome Engineering in Industrial Yeast Strains

SBIR 第二阶段：工业酵母菌株的超快速基因组工程

• 批准号: 1430813
• 负责人: Noah Taylor
• 金额: $ 60.87万
• 依托单位: Enevolv, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-11-15 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1430813&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Ultra; Rapid

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is the ability to rapidly engineer yeast for production of fuels, chemicals, enzymes, and other valuable molecules. Yeast offers high value production capabilities; however, engineering new strains is very complex and expensive - costing approximately $75-100 million, and requiring 7-10 years for development with current engineering methods. This project will develop a genome engineering technology for yeast that can substantially reduce the cost and time for developing new strains for industrial biotechnology applications. This technology will increase the ability of companies to use yeast to efficiently produce high-quality products from renewable feedstocks, and help grow the overall industrial biotechnology market. This SBIR Phase II project proposes to develop the ability to perform rapid whole-genome engineering in industrial yeast strains. Current technologies to engineer cells for specific functions (e.g., chemical or fuel production) are inefficient, expensive and extremely time consuming. Multiplex Automated Genome Engineering (MAGE) is a disruptive technology that provides a powerful platform to engineer microorganisms at tremendously reduced cost. Until recently, MAGE was limited to a laboratory strain of E. coli, possessing key genetic features that enable MAGE. Recent advances have allowed us to port MAGE to yeast, and the goal of this project is to increase efficiency of the system and endow existing industrial yeasts with the capacity to undergo MAGE. The goal is to improve MAGE efficiency in yeast by developing a high-throughput system to rapidly create and quantify strain variants for their capacity to undergo the MAGE process itself. This project will establish a system to identify and optimize the requisite genetic features for MAGE in yeast, in order to rapidly engineer industrial yeast strains for specific function.

这个小企业创新研究（SBIR）第二阶段项目的更广泛的影响/商业潜力是能够快速工程酵母生产燃料，化学品，酶和其他有价值的分子。酵母提供了高价值的生产能力;然而，工程新菌株是非常复杂和昂贵的-花费约75 - 1亿美元，并需要7-10年的发展与目前的工程方法。该项目将开发一种酵母基因组工程技术，可以大大减少开发工业生物技术应用新菌株的成本和时间。这项技术将提高公司使用酵母从可再生原料中有效生产高质量产品的能力，并有助于发展整个工业生物技术市场。 该SBIR第二阶段项目旨在开发在工业酵母菌株中进行快速全基因组工程的能力。目前工程化细胞以实现特定功能的技术（例如，化学品或燃料生产）是低效的、昂贵的和极其耗时的。多重自动基因组工程（法师）是一种颠覆性的技术，它提供了一个强大的平台，以极大地降低成本来工程化微生物。直到最近，法师仅限于实验室菌株的E。大肠杆菌，具有关键的遗传特征，使法师。最近的进展使我们能够将法师移植到酵母中，本项目的目标是提高系统的效率，并赋予现有的工业酵母进行法师的能力。目标是通过开发高通量系统以快速产生和定量菌株变体以使其自身经历法师过程的能力来提高法师在酵母中的效率。本计画将建立一套系统，以鉴定并优化酵母中法师所需的遗传特性，以快速工程化工业酵母菌株，使其具有特定的功能。