SBIR Phase II: Real-Time Biosensor for Measuring Hazardous Chemical Contaminants in Ground Water

SBIR 第二阶段：用于测量地下水中有害化学污染物的实时生物传感器

• 批准号: 1431003
• 负责人: Brian Heinze
• 金额: $ 74.68万
• 依托单位: OptiEnz Sensors
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-01 至 2018-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1431003&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Real; Time

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project is to increase the efficiency of identifying, mapping, and remediating water sources contaminated with chlorinated solvents. Chlorinated solvents are used in vast quantities as solvents and degreasing agents, and in the dry cleaning industry. The necessity of monitoring chlorinated solvents in water will continue for many generations due to their widespread occurrence in water sources around the world, their toxicity, and their high stability. A significant need exists and will grow for devices that can continuously measure the concentration of chlorinated organic chemicals in water, and that can do so accurately and inexpensively. Continuous in-situ data production in the field of environmental sensing will greatly increase efficiency in all processes that require a measurement of these chemicals, having a significant impact on the costs and timelines associated with remediation of contaminated sites. This sensor will allow for continuous monitoring of possible contamination sources in the case of a contamination event. Also, it will afford easy profiling of contamination plumes in aquifers. This technology will replace the current laboratory-based measurement protocols, which are not readily adapted for in-situ, on-line, or in-the-field measurements, and cannot produce data continuously.This SBIR Phase II project proposes to develop an optical enzymatic biosensor for continuous, quantitative monitoring of chlorinated solvent concentrations in water. Chlorinated solvent contamination of groundwater is a widespread ongoing issue around the world. The biosensing concept will be implemented in an optical fiber format, in which optical biosensor tips are linked to a small electro-optical unit. Analyte detection takes place on biosensor tips that are coated with enzymes and fluorescent chemicals. The scope of the research includes two parallel strategies for obtaining highly stable detection enzymes such that long lifetime sensors are achievable, development of a system to deliver required reagents to the sensing tip, development of signal processing algorithms to enable high accuracy measurements in variable environmental conditions and speciation of chlorinated compounds within mixtures, a manufacturing study for high throughput fabrication of the sensors, and field testing of the device at multiple contamination sites. Attainment of these objectives will advance the sensing technology to a robust and readily manufactured system for chlorinated solvent monitoring with low detection limits, high accuracy, and reliability.

小企业创新研究（SBIR）第二阶段项目的更广泛影响/商业潜力是提高识别、测绘和修复受氯化溶剂污染的水源的效率。 氯化溶剂被大量用作溶剂和消毒剂，并用于干洗行业。由于水中氯化溶剂在世界各地水源中的广泛存在、其毒性和高稳定性，监测水中氯化溶剂的必要性将持续许多代。对于能够连续测量水中氯化有机化学品的浓度并且能够准确且廉价地进行测量的装置存在显著需求并且将增长。在环境传感领域连续的现场数据生产将大大提高需要测量这些化学品的所有过程的效率，对污染场地补救的成本和时间表产生重大影响。该传感器将允许在污染事件的情况下连续监测可能的污染源。此外，它还将提供对含水层中污染羽流的简单剖析。这项技术将取代目前的实验室为基础的测量协议，这是不容易适应的原位，在线，或在现场的测量，并不能产生连续的data.This SBIR第二阶段项目建议开发一个光学酶生物传感器连续，定量监测水中的氯化溶剂浓度。氯化溶剂对地下水的污染是世界各地普遍存在的问题。生物传感概念将以光纤形式实现，其中光学生物传感器尖端连接到小型电光单元。分析物检测发生在涂有酶和荧光化学物质的生物传感器尖端上。研究范围包括两种并行策略，用于获得高度稳定的检测酶，以便实现长寿命传感器，开发系统以将所需试剂输送到传感尖端，开发信号处理算法以实现在可变环境条件下的高精度测量和混合物中氯化化合物的形态，传感器高通量制造的制造研究，以及在多个污染部位对装置进行现场测试。这些目标的实现将推动传感技术成为一个强大的、易于制造的系统，用于氯化溶剂监测，具有低检测限、高准确度和可靠性。