Evaluation of the Role of Size Dispersity on Nanoparticle Uptake and Ecotoxicity

尺寸分散性对纳米颗粒吸收和生态毒性作用的评估

• 批准号: 1437307
• 负责人: Mohammed Baalousha
• 金额: $ 29.99万
• 依托单位: University of South Carolina at Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1437307&HistoricalAwards=false
• 关键词: Evaluation; Role; Size; Dispersity; Nanoparticle

PI: Baalousha, MohammedProposal Number: 1437307 Institution: University of South Carolina at Columbia Title: Evaluation of the Role of Size Dispersity on Nanoparticle Uptake and EcotoxicityStudies of nanoparticle (NP) toxicity to the environment often come up with contradictory results. For example, nanoparticles (NPs) have demonstrable negative impact on aquatic organisms, but divergent results have been reported on the role of NP size on their toxicity. The PI and Co-PIs hypothesize that such contradictory evidence is most likely due to the heterogeneity and polydispersity of the NPs themselves. They hypothesize that polydispersity can cause incorrect measurement of NP properties and lead to an underestimation of NP dose, particularly if the toxic effects are only due to a sub-fraction of the polydisperse suspensions. The proposal will specifically investigate the effect of NP dispersity on their properties and on their toxicities, investigating such NP properties as size, dispersity, morphology, and facet exposure, and their effect in nanotoxicity. The results of this project will help improve the design of NPs by identifying features that can induce deleterious effects, and also help Understand the fate, behavior, uptake, and toxicity of NPs. Progress in this area will aid a sustainable development of nanotechnology and its benefits. Under this proposal's plan, the PIs will work directly with K-12 institutions and underrepresented minorities (URMs) in South Carolina and produce distance-learning materials for wider outreach. The PIs have a strong track record of ensuring inclusion of under-represented groups in training and outreach activities and will recruit for the PhD candidate with outreach principles in mind. The University of South Carolina has one of the highest numbers of African American students for a Carnegie One research intensive university (13%).To achieve their goals, the PIs must first rigorously characterize the NPs they synthesize and then correlate the various properties of the NPs with their observed toxicities. To that end, the PIs will seek to establish a reproducible protocol for the synthesis of poly- and mono-dispersed NPs and their separation into a set of monodisperse subfractions. They will then characterize the NPs (e.g., size, dispersity, morphology, facets) using a multi-method approach and quantify the uptake and toxicity of polydisperse versus monodisperse subfractions of NPs using the standardized polyhaline estuarine copepod model, Amphiascus tenuiremis.With this approach, the PI and Co-PIs seek to provide a methodology that can be widely used by the scientific community for similar and other studies, as well as in other fields such as drug delivery, where NP size dispersity has been realized as an issue of confounding results in the literature.

主要研究者：Baalousha，Mohammed提案编号：1437307机构：哥伦比亚的南卡罗来纳州大学标题：尺寸分散性对纳米颗粒吸收和生态毒性作用的评估纳米颗粒（NP）对环境毒性的研究经常得出相互矛盾的结果。例如，纳米颗粒（NPs）对水生生物有明显的负面影响，但关于纳米颗粒大小对其毒性的作用，已有不同的结果报道。PI和Co-PI假设这种矛盾的证据很可能是由于NP本身的异质性和多分散性。他们假设多分散性可能导致NP性质的不正确测量，并导致NP剂量的低估，特别是如果毒性作用仅归因于多分散悬浮液的亚部分。该提案将专门研究NP分散性对其性质和毒性的影响，研究NP的大小，分散性，形态和小面暴露等性质，以及它们在纳米毒性中的作用。该项目的结果将有助于通过识别可能引起有害影响的特征来改进NP的设计，并有助于了解NP的命运，行为，摄取和毒性。这一领域的进展将有助于纳米技术及其益处的可持续发展。根据该提案的计划，PI将直接与南卡罗来纳州的K-12机构和代表性不足的少数民族（URM）合作，并制作远程学习材料，以进行更广泛的宣传。PI在确保将代表性不足的群体纳入培训和外联活动方面有着良好的记录，并将在考虑到外联原则的情况下招聘博士候选人。南卡罗来纳州大学是卡内基一号研究密集型大学中非洲裔美国学生人数最多的大学之一（13%）。为了实现他们的目标，PI必须首先严格描述他们合成的NP，然后将NP的各种性质与观察到的毒性相关联。为此，PI将寻求建立一种可重复的方案，用于合成多分散和单分散的NP并将其分离成一组单分散亚组分。然后，他们将表征NP（例如，大小，分散性，形态，小平面），并使用标准化的多盐河口桡足类模型，Amphiacetabenuiremis量化多分散与单分散纳米颗粒亚组分的吸收和毒性。通过这种方法，PI和Co-PI寻求提供一种方法，可以广泛用于科学界的类似和其他研究，以及其他领域，如药物输送，其中NP尺寸分散度在文献中被认为是混淆结果的问题。

项目成果

Particle size determines the accumulation of platinum nanoparticles in the estuarine amphipod, Leptocheirus plumulosus

粒径决定铂纳米颗粒在河口端足类动物 Leptocheirus penulosus 中的积累

• DOI: 10.1039/d1en00713k
• 发表时间: 2022
• 期刊: Environmental Science: Nano
• 作者: Sikder, Mithun;Eudy, Emily;Cai, Bo;Chandler, G. Thomas;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

The concentration-dependent aggregation of Ag NPs induced by cystine

• DOI: 10.1016/j.scitotenv.2016.02.212
• 发表时间: 2016-07-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, K.;Gibson, I.;Baalousha, M.
• 通讯作者: Baalousha, M.

Effect of phosphate buffer on aggregation kinetics of citrate-coated silver nanoparticles induced by monovalent and divalent electrolytes

• DOI: 10.1016/j.scitotenv.2016.12.117
• 发表时间: 2017-03-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, K.;Baalousha, M.
• 通讯作者: Baalousha, M.

Synthesis, characterization, and environmental behaviors of monodispersed platinum nanoparticles

• DOI: 10.1016/j.jcis.2019.01.036
• 发表时间: 2019-03-22
• 期刊: JOURNAL OF COLLOID AND INTERFACE SCIENCE
• 影响因子: 9.9
• 作者: Sikder, Mithun;Wang, Jingjing;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

Potential impact of natural organic ligands on the colloidal stability of silver nanoparticles

• DOI: 10.1016/j.scitotenv.2017.12.299
• 发表时间: 2018-06-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, Kamelia;Marrone, Brandon;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed