Deciphering the role of RNA binding proteins in RNA transport, localization and post-transcriptional processes in plants

到2050年，世界人口预计将增加到90亿以上，这就需要增加70%的粮食供应。要实现这一目标，就必须增加主要作物的产量，特别是谷物，因为谷物以蛋白质和淀粉的形式满足了世界上很大一部分人口的大量营养需求。这项研究的结果将提供新的见解和创新策略，通过操纵参与生产储存在稻米中的蛋白质和淀粉的早期步骤的酶（促进化学反应的蛋白质）来提高作物产量。此外，该项目将提供分子、细胞和遗传方法方面的最新研究培训，特别是为博士后、研究生和本科生，包括来自一个为少数群体服务的伙伴机构-洛杉矶的加州州立大学的学生提供大规模蛋白质（蛋白质组学）和基因（基因组学）研究培训。这项研究的成果将被整合到一个关于分子运动的新网站上（例如RNA和蛋白质如何在植物细胞内移动到它们发挥功能的位点）是现代植物生物学教科书中描述得很少的重要主题。尽管多年来已经产生了大量关于植物基因如何在转录和转录后水平上被控制的信息，关于RNA如何被运输和定位到植物细胞的特定亚区室，在那里它们被翻译、储存、加工或翻转，人们知之甚少。这项研究将集中在运输和定位的RNA在发育中的水稻胚乳的目的是提供一个全面的图片的RNA结合蛋白（RBP）参与，它们是如何组织成多RBP复合物，以及如何RBP可能是不同的RNA运输途径的共同因素，然而，提供方向的RNA靶向特定的个人途径。该项目将使用全基因组方法来确定特定RBP在RNA运输和定位到皮质内质网中的作用，以及它们在翻译，稳定性和/或RNA加工中的后续作用。具体目标是：（1）将细胞质定位的、细胞质相关的RBP分类为多蛋白复合物;（2）确定这些多蛋白复合物结合的RNA的组成，并鉴定相关的顺式序列;（3）通过分析基因突变，确定可能负责指定特定RNA转运途径的特定RBP的模块结构域的作用。 这些信息将为RNA的命运及其在植物细胞中的位置提供前所未有的观点，从而填补了我们对转录以外基因表达步骤的理解的主要空白。

The world population is projected to increase to over 9 billion people by 2050, necessitating a 70% increase in the food supply. Such a goal can only be accomplished by increasing the yields of the major crop plants, especially the cereals, whose grains meet substantial nutritional needs in the form of protein and starch for a significant portion of the world's population. Results from this research will provide new insights and innovative strategies to improve crop yields by manipulating the enzymes (proteins that facilitate chemical reactions) involved in the early steps of the production of the proteins and starch that are stored in rice grains. In addition, this project will provide state-of-the-art research training in molecular, cellular and genetic methods, especially large-scale studies of proteins (proteomics) and genes (genomics) for postdoctoral, graduate and undergraduate students, including students from a partnering minority-serving institution, California State University at Los Angeles. Products of this research will be integrated into a new website on molecular kinesis (how for example RNA and proteins move within plant cells to the sites where they function) an important topic that is poorly described in modern plant biology textbooks.Although considerable information has been generated over the years on how plant genes are controlled at the transcriptional and post-transcriptional levels, very little is known about how RNAs are transported and localized to specific sub-compartments of the plant cell where they are translated, stored, processed or turned-over. This research will focus on the transport and localization of RNAs in developing rice endosperm with the aim of providing a comprehensive picture of the RNA binding proteins (RBPs) involved, how they are organized into multi-RBP complexes, and how RBPs that may be common factors of distinct RNA transport pathways may, nevertheless, provide the directions for targeting RNAs to particular individual pathways. This project will use genome-wide approaches to identify the role of specific RBPs in the transport and localization of RNAs to the cortical endoplasmic reticulum and their subsequent roles in translation, stability and/or RNA processing. Specific objectives will be to: (1) categorize cytoplasmic-localized, cytoskeletal-associated RBPs into multi-protein complexes; (2) determine the composition of RNAs bound by these multi-protein complexes and identify the associated cis-sequences; and (3) determine the role of modular domains of specific RBPs that may be responsible for specifying a particular RNA transport pathway by the analysis of genetic mutations. This information will provide an unprecedented view of the fate of RNAs and their location in the plant cell and will thereby fill a major gap in our understanding of steps in gene expression beyond transcription.