Control of Storage Protein Biosynthesis by mRNA Targeting

植物种子被用作食物来源和生产生物能源的原料。到2050年，世界人口预计将超过90亿，因此必须生产比以往更多的粮食。尽管在了解种子发育过程中种子基因是如何在细胞核中调控的方面已经付出了相当大的努力，但对细胞质中控制其rna表达的细胞事件知之甚少。该实验室之前的研究表明，种子rna在离开细胞核时不会立即翻译成蛋白质。相反，它们形成大颗粒，沿着几种不同的途径运输到皮质区，皮质区位于质膜下方，非常有利于蛋白质合成。在这个项目中，将采用综合分子、遗传和细胞成像方法来阐明RNA转运和定位的三个广泛领域。它们是(1)鉴定被某一运输途径定位的RNA种类，(2)鉴定和表征特定RNA结合蛋白如何相互作用以识别其靶RNA并将其引导到特定的RNA运输途径，以及(3)确定膜相关蛋白在RNA运输中的作用。这项研究将为rna和蛋白质如何在植物细胞中定位和积累提供新的见解。此外，它可能导致对控制碳和氮的利用及其转化为储存储量的机制的新见解，从而有助于提高种子质量和作物生产力。该项目将培养博士后、研究生和本科水平的年轻科学家，将为这些年轻科学家提供进行国际研究的机会，并将加强正在进行的国际合作研究，以增加粮食和能源种子的产量。

Plant seeds are used as a source of food and as a feedstock for the production of bioenergy. As the world population is projected to surpass 9 billion people by 2050, more food will have to be produced than ever before. Although considerable effort has been directed at understanding how seed genes are regulated in the nucleus during seed development, very little is known about the cellular events that control the expression of their RNAs in the cytoplasm. Previous efforts from this laboratory have demonstrated that seed RNAs are not immediately translated into protein when they exit the nucleus. Instead, they form large particles which are transported along several different pathways to the cortical region, a site located underneath the plasma membrane and highly conducive for protein synthesis. In this project, an integrated molecular, genetic and cell imaging approach will be undertaken to elucidate three broad areas of RNA transport and localization. They are (1) to identify the RNA species, which are localized by one of the transport pathways, (2) to identify and characterize how specific RNA binding proteins interact to recognize their target RNAs and direct them to a specific RNA transport pathway, and (3) to determine the role of membrane-associated proteins in RNA transport. This study will provide new insights in how RNAs and, in turn, proteins are localized and accumulated in plant cells. Moreover, it may lead to new insights into the mechanisms that control the utilization of carbon and nitrogen and their conversion into storage reserves and, thereby, aid in efforts to increase seed quality and crop productivity. The project will train young scientists at the postdoctoral, graduate and undergraduate levels, will provide these younger scientists the opportunity for international research, and will strengthen an ongoing international collaborative study to increase seed production for food and energy.