A Resource for Functional Genomics to Support Soybean Genetics and Breeding

支持大豆遗传学和育种的功能基因组学资源

• 批准号: 1444581
• 负责人: Wayne Parrott
• 金额: $ 343.25万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-15 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1444581&HistoricalAwards=false
• 关键词: Resource; Functional; Genomics; Support; Soybean

PI: Peter LaFayette (University of Georgia)coPIs: Robert Stupar (University of Minnesota), Minviluz Stacey (University of Missouri), Thomas E. Clemente (University of Nebraska), and C. Nathan Hancock (University of South Carolina - Aiken)Soybeans provide the single greatest source of vegetable protein and oil worldwide, and uses of this important crop now range from human food to alternatives for petroleum-derived chemicals. The goal of this project is to create molecular tools to make it easy for geneticists and breeders to identify and study the function of genes associated with desirable agronomic traits. The project will provide laboratory and field research opportunities for postdoctoral fellows and undergraduate students, including students from a predominantly undergraduate institution. In addition, yearly workshops will bring together soybean researchers from across the nation to participate in hands-on training in precision genomics and in screening plant populations to identify new traits of interest. The resources developed by this project will advance soybean improvement, which in turn can lead to designer oils and proteins for healthier diets, industrial compounds, and biodiesel to meet a growing global demand.This research will add to the current set of forward and reverse genetics resources available to the plant research community. A key activity will be to develop and characterize the publicly available soybean fast neutron (FN) mutagenesis population with a goal of attaining a biologically relevant amount of genomic coverage. Concurrently, individual genes within FN deleted regions that alter traits of importance to the soybean community (e.g., yield, oil, protein, stress tolerance, and nitrogen fixation) will be mutated using CRISPER/Cas9 technology to pinpoint the genes responsible for specific phenotypes. In addition, a set of complementary, transposon-based mutagenesis populations will be made to specifically tailor gene discovery for the highly duplicated genome of soybean. TheTnt1 insertional mutagenesis population will result in traditional loss-of-function phenotypes, the Ac/Ds activation tagging population will cause gene mis-expression, and the mPing transposon will be used for both gene silencing and overexpression. These strategies are expected to result in recovery of novel phenotypes not previously observed in soybean. Data, phenotypes, seeds and other information will be available via the project website at http://soymutants.uga.edu and at SoyBase.org.

PI：Peter LaFayette（格鲁吉亚大学）coPI：Robert Stupar（明尼苏达大学），Minviluz Stacey（密苏里州大学），托马斯E。Clemente（内布拉斯加大学）和C. Nathan汉考克（南卡罗来纳州- Aiken大学）菜豆是全世界植物蛋白和油的最大来源，这种重要作物的用途现在从人类食物到石油衍生化学品的替代品。 该项目的目标是创建分子工具，使遗传学家和育种家能够轻松识别和研究与理想农艺性状相关的基因的功能。该项目将为博士后研究员和本科生提供实验室和实地研究机会，包括来自以本科为主的机构的学生。此外，每年的研讨会将汇集来自全国各地的大豆研究人员，参加精确基因组学和筛选植物种群以确定感兴趣的新性状的实践培训。 该项目开发的资源将促进大豆改良，从而可以为更健康的饮食、工业化合物和生物柴油提供设计师油和蛋白质，以满足不断增长的全球需求。该研究将增加植物研究界现有的正向和反向遗传学资源。一项关键活动将是开发和表征公开可用的大豆快中子（FN）诱变群体，目标是获得生物相关量的基因组覆盖率。 同时，FN缺失区域内改变大豆群落重要性状的单个基因（例如，产量，油，蛋白质，胁迫耐受性和固氮）将使用CRISPER/Cas9技术进行突变，以确定负责特定表型的基因。此外，一组互补的，转座子为基础的诱变群体将专门定制的基因发现的高度重复的基因组的大豆。Tnt 1插入突变群体将导致传统的功能丧失表型，Ac/Ds激活标签群体将导致基因错误表达，而mPing转座子将用于基因沉默和过表达。这些策略有望恢复以前在大豆中未观察到的新表型。 数据、表型、种子和其他信息将通过项目网站http://soymutants.uga.edu和http：//www.example.org提供。

项目成果

A partial T-DNA insertion near KNAT1 results in lobed Arabidopsis thaliana leaves

KNAT1 附近的部分 T-DNA 插入导致拟南芥叶片呈浅裂状

• DOI: 10.17912/micropub.biology.000253
• 发表时间: 2020
• 期刊: microPublication biology
• 作者: Moulton, K;Diaz, S;Strother, A;Hancock, CN
• 通讯作者: Hancock, CN

Targeted Insertion of the mPing Transposable Element

mPing 转座元件的定向插入

• 发表时间: 2018
• 期刊: Journal of the South Carolina Academy of Science
• 作者: Strother, Ashley E.;Diaz, Stephanie S.;Baker, Mary E.;and Hancock, C. Nathan
• 通讯作者: and Hancock, C. Nathan