A Resource for Functional Genomics to Support Soybean Genetics and Breeding

合作伙伴：Robert Stupar（明尼苏达大学），Minviluz Stacey（密苏里大学），Thomas E. Clemente（内布拉斯加州大学）和C. Nathan Hancock（南卡罗莱纳大学-艾肯分校）大豆是世界范围内植物蛋白和油的最大单一来源，这种重要作物的用途现在从人类食品到石油衍生化学品的替代品。该项目的目标是创建分子工具，使遗传学家和育种家能够轻松地识别和研究与理想农艺性状相关的基因功能。该项目将为博士后和本科生提供实验室和实地研究的机会，包括来自主要本科院校的学生。此外，一年一度的研讨会将把来自全国各地的大豆研究人员聚集在一起，参加精确基因组学和筛选植物种群以确定感兴趣的新性状的实践培训。该项目开发的资源将促进大豆的改良，进而可以为更健康的饮食、工业化合物和生物柴油提供设计油和蛋白质，以满足日益增长的全球需求。这项研究将为植物研究界增加现有的正向和反向遗传资源。一项关键活动将是开发和表征公开可用的大豆快中子（FN）诱变群体，目标是获得生物学上相关的基因组覆盖率。同时，使用CRISPER/Cas9技术，在FN缺失区域内改变对大豆群落重要性状（如产量、油脂、蛋白质、耐受性和固氮性）的单个基因将发生突变，以确定对特定表型负责的基因。此外，将建立一套互补的、基于转座子的突变群体，以专门为大豆高度重复的基因组量身定制基因发现。tnt1插入突变群体将导致传统的功能缺失表型，Ac/Ds激活标记群体将导致基因错误表达，mPing转座子将用于基因沉默和过表达。这些策略有望恢复以前未在大豆中观察到的新表型。数据、表型、种子和其他信息将通过项目网站http://soymutants.uga.edu和SoyBase.org获得。

PI: Peter LaFayette (University of Georgia)coPIs: Robert Stupar (University of Minnesota), Minviluz Stacey (University of Missouri), Thomas E. Clemente (University of Nebraska), and C. Nathan Hancock (University of South Carolina - Aiken)Soybeans provide the single greatest source of vegetable protein and oil worldwide, and uses of this important crop now range from human food to alternatives for petroleum-derived chemicals. The goal of this project is to create molecular tools to make it easy for geneticists and breeders to identify and study the function of genes associated with desirable agronomic traits. The project will provide laboratory and field research opportunities for postdoctoral fellows and undergraduate students, including students from a predominantly undergraduate institution. In addition, yearly workshops will bring together soybean researchers from across the nation to participate in hands-on training in precision genomics and in screening plant populations to identify new traits of interest. The resources developed by this project will advance soybean improvement, which in turn can lead to designer oils and proteins for healthier diets, industrial compounds, and biodiesel to meet a growing global demand.This research will add to the current set of forward and reverse genetics resources available to the plant research community. A key activity will be to develop and characterize the publicly available soybean fast neutron (FN) mutagenesis population with a goal of attaining a biologically relevant amount of genomic coverage. Concurrently, individual genes within FN deleted regions that alter traits of importance to the soybean community (e.g., yield, oil, protein, stress tolerance, and nitrogen fixation) will be mutated using CRISPER/Cas9 technology to pinpoint the genes responsible for specific phenotypes. In addition, a set of complementary, transposon-based mutagenesis populations will be made to specifically tailor gene discovery for the highly duplicated genome of soybean. TheTnt1 insertional mutagenesis population will result in traditional loss-of-function phenotypes, the Ac/Ds activation tagging population will cause gene mis-expression, and the mPing transposon will be used for both gene silencing and overexpression. These strategies are expected to result in recovery of novel phenotypes not previously observed in soybean. Data, phenotypes, seeds and other information will be available via the project website at http://soymutants.uga.edu and at SoyBase.org.