CAREER: SELECTIVE TRANSPORT IN BIOLOGICAL HYDROGELS - FROM DESIGN PRINCIPLES TO MECHANISMS
职业:生物水凝胶中的选择性运输 - 从设计原理到机制
基本信息
- 批准号:1454673
- 负责人:
- 金额:$ 72.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-01 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Hydrogels in Biology represent a class of molecular assemblies that are understudied, with many fascinating and important structure-function problems to discover and engineering applications to invent. Nuclear pores, for example, the tiny channels in the nuclear envelope, are filled with a hydrogel that controls the selective exchange of molecules between the nucleus and the cytoplasm. Another important biogel is mucus, made from highly hydrated biopolymers from a ombination of peptide and sugar subunits. Mucus coats all the wet surfaces in the human body, providing a selective barrier that allows nutrients and information in while keeping pathogens out. Hydrogels are an integral part of Biology and, yet, clear concepts of how they function are missing. The goal of this project is to elucidate the fundamental biophysical principles that allow biogels to act as selective barriers and filters, and the cellular mechanisms involved in building and regulating them. The project also will educate and engage community health leaders, teachers and students about the state-of-the-art science of hydrogels, emphasizing the importance of mucus barriers within the broader context of the Physics of nature's bio-materials. The research will also be the cornerstone for the development of free online teaching material, including a children's book and a video contest, enabling widespread public awareness and understanding of biological hydrogels. The nuclear pore filter is an ideal system in which to elucidate general principles of biofiltration by hydrogels. Its molecular composition is well characterized, and can also be reconstituted from well-defined phenylalanine-rich repeat units. A unique suite of quantitativ methods and theoretical tools will be used to characterize selective transport and to elucidate the relationship between a particle's biochemical properties, and its mobility across nuclear pore hydrogel barriers. The project will also engineer hydrogel filters with a precise control of selected elements of the barrier, including the size, the charge, and the cross-link chemistry of the repeat units. The PI's aim is to substantially extend the already existing technology to study hydrogelbased filtering, and advance the field toward new frontiers, by addressing the function of repeat domains, the basic building blocks of many biological gels, in the regulation of barrier selectivity. Her goal is to provide the foundation for a theoretical framework that captures general principles governing selectivity in the nuclear pore, and likely also in other important biological hydrogels such as the mucus, and bacterial biofilms.
水凝胶在生物学中代表了一类未被研究的分子组合,有许多有趣而重要的结构-功能问题有待发现,工程应用有待发明。核孔,例如核膜中的微小通道,被一种水凝胶填充,这种水凝胶控制着细胞核和细胞质之间的选择性分子交换。另一种重要的生物凝胶是粘液,由多肽和糖亚单位组合而成的高度水合的生物聚合物制成。粘液覆盖在人体所有潮湿的表面,提供了一种选择性屏障,允许营养物质和信息进入,同时将病原体拒之门外。水凝胶是生物学不可分割的一部分,然而,关于它们如何发挥作用的明确概念尚不清楚。该项目的目标是阐明生物凝胶作为选择性屏障和过滤器的基本生物物理原理,以及构建和调节它们的细胞机制。该项目还将教育和吸引社区卫生领导人、教师和学生了解水凝胶的最新科学,强调粘液屏障在自然生物材料物理学的更广泛背景下的重要性。这项研究也将成为开发免费在线教材的基石,包括一本儿童书籍和一场视频比赛,使公众能够广泛了解生物水凝胶。核孔过滤器是阐明水凝胶生物过滤一般原理的理想系统。它的分子组成具有很好的特性,也可以由定义明确的富含苯丙氨酸的重复单元重组。将使用一套独特的定量方法和理论工具来表征选择性运输,并阐明粒子的生化特性与其跨越核孔水凝胶屏障的移动性之间的关系。该项目还将设计水凝胶过滤器,精确控制屏障的选定元素,包括重复单元的大小、电荷和交联化学。PI的目的是通过解决重复结构域在调节屏障选择性中的作用,大幅扩展现有的水凝胶过滤研究技术,并将该领域推向新的前沿。重复结构域是许多生物凝胶的基本构件。她的目标是为一个理论框架提供基础,该框架捕捉到控制核孔选择性的一般原则,可能还包括其他重要的生物水凝胶,如粘液和细菌生物膜。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Engineering mucus to study and influence the microbiome
- DOI:10.1038/s41578-018-0079-7
- 发表时间:2019-02
- 期刊:
- 影响因子:83.5
- 作者:Caroline A. Werlang;Gerardo Cárcarmo-Oyarce;K. Ribbeck
- 通讯作者:Caroline A. Werlang;Gerardo Cárcarmo-Oyarce;K. Ribbeck
Molecular Characterization of Mucus Binding
粘液结合的分子表征
- DOI:10.1021/acs.biomac.8b01467
- 发表时间:2019
- 期刊:
- 影响因子:6.2
- 作者:Witten, Jacob;Samad, Tahoura;Ribbeck, Katharina
- 通讯作者:Ribbeck, Katharina
Swimming bacteria promote dispersal of non-motile staphylococcal species
游动细菌促进非运动葡萄球菌物种的扩散
- DOI:10.1038/ismej.2017.23
- 发表时间:2017
- 期刊:
- 影响因子:0
- 作者:Samad, Tahoura;Billings, Nicole;Birjiniuk, Alona;Crouzier, Thomas;Doyle, Patrick S;Ribbeck, Katharina
- 通讯作者:Ribbeck, Katharina
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Katharina Ribbeck其他文献
MIT Open Access Articles Biological hydrogels as selective diffusion barriers
麻省理工学院开放获取文章生物水凝胶作为选择性扩散屏障
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
Oliver Lieleg;Katharina Ribbeck - 通讯作者:
Katharina Ribbeck
Mucus
粘液
- DOI:
10.1002/0471743984.vse5011 - 发表时间:
2021 - 期刊:
- 影响因子:9.2
- 作者:
Abigail McShane;J. Bath;A. M. Jaramillo;Caroline Ridley;A. Walsh;C. Evans;D. Thornton;Katharina Ribbeck - 通讯作者:
Katharina Ribbeck
Prevotella are major contributors of sialidases in the human vaginal microbiome
普雷沃氏菌是人类阴道微生物群中唾液酸酶的主要贡献者
- DOI:
10.1101/2024.01.09.574895 - 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Paula Pelayo;Fatima A. Hussain;Caroline A. Werlang;Chloe Wu;B. Woolston;Katharina Ribbeck;Douglas S. Kwon;E. Balskus - 通讯作者:
E. Balskus
MIT Open Access Articles Mucin Biopolymers As Broad-Spectrum Antiviral Agents
麻省理工学院开放获取文章粘蛋白生物聚合物作为广谱抗病毒剂
- DOI:
10.1108/jfbm-09-2018-0037 - 发表时间:
2019 - 期刊:
- 影响因子:2.7
- 作者:
Oliver Lieleg;Corinna Lieleg;Joan V. Bloom;Christopher B. Buck;Katharina Ribbeck - 通讯作者:
Katharina Ribbeck
Electrostatic Interactions Control the Permeability of Mucin Hydrogels
- DOI:
10.1016/j.bpj.2009.12.337 - 发表时间:
2010-01-01 - 期刊:
- 影响因子:
- 作者:
Oliver Lieleg;Ioana Vladescu;Katharina Ribbeck - 通讯作者:
Katharina Ribbeck
Katharina Ribbeck的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Katharina Ribbeck', 18)}}的其他基金
Collaborative Research: MIM: Learning how mucus shapes and maintains microbiomes
合作研究:MIM:了解粘液如何塑造和维持微生物组
- 批准号:
2125118 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
RAPID: Identifying the Role of Mucus in COVID-19 Pathogenesis
RAPID:确定粘液在 COVID-19 发病机制中的作用
- 批准号:
2033046 - 财政年份:2020
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
相似海外基金
Regulation of selective endocytic transport
选择性内吞转运的调节
- 批准号:
2313900 - 财政年份:2023
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
Collaborative Research: Crossing the percolation threshold for selective gas transport using interconnected crystals of metal–organic frameworks in polymer-based hybrid membranes
合作研究:利用聚合物杂化膜中金属有机框架的互连晶体跨越选择性气体传输的渗滤阈值
- 批准号:
2034742 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
Defining the Mechanistic Determinants of Response to Selective Electron Transport Chain Inhibition in Cancer
定义癌症选择性电子传递链抑制反应的机制决定因素
- 批准号:
10154758 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
2-dimensional Borophene Sheets for Selective Proton Transport
用于选择性质子传输的二维硼烯片
- 批准号:
21K14710 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Defining the Mechanistic Determinants of Response to Selective Electron Transport Chain Inhibition in Cancer
定义癌症选择性电子传递链抑制反应的机制决定因素
- 批准号:
10362553 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Understanding and Controlling Ionic Behaviors in Heterostructured Metal-Organic-Frameworks for Selective Magnesium Ion Transport
了解和控制异质结构金属有机框架中的离子行为以实现选择性镁离子传输
- 批准号:
2126923 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Continuing Grant
NSF-BSF: Selective Transport of Divalent Cations through Polymeric Membranes Using Host-Guest Chemistry
NSF-BSF:利用主客体化学选择性通过聚合物膜传输二价阳离子
- 批准号:
2110138 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
Collaborative Research: Crossing the percolation threshold for selective gas transport using interconnected crystals of metal–organic frameworks in polymer-based hybrid membranes
合作研究:利用聚合物杂化膜中金属有机框架的互连晶体跨越选择性气体传输的渗滤阈值
- 批准号:
2034734 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Standard Grant
Novel Cell Function Control Using Plasma Selective Transport and Radical Reaction Delay under Tesla-class Strong Magnetic Field
特斯拉级强磁场下利用等离子体选择性传输和自由基反应延迟控制新型细胞功能
- 批准号:
21K18613 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Computer Led Design of Helical Arylamide Foldamer for Selective Water Transport
用于选择性水传输的螺旋芳酰胺折叠体的计算机设计
- 批准号:
2205220 - 财政年份:2021
- 资助金额:
$ 72.5万 - 项目类别:
Continuing Grant