Collaborative Research: Relating detrital peptide sequences to particulate organic matter diagenetic histories
合作研究:将碎屑肽序列与颗粒有机质成岩历史联系起来
基本信息
- 批准号:1458017
- 负责人:
- 金额:$ 39.92万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-02-15 至 2019-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Biological, chemical, and physical processes alter recently deposited marine sediments. During this alteration, commonly referred to as early diagenesis, the quantity and composition of organic matter is changed which influences key components of the marine carbon cycle. In this study, researchers will use analytical techniques to evaluate the geochemistry of peptides during early diagenesis, and develop methods for assessing the role bacteria play. This research is important because organic molecules such as peptides influence degradation rates and the fate of carbon and nitrogen in the ocean, and therefore have broad relevance for understanding the geochemical cycling of these key nutrients. The project will also involve research opportunities for undergraduate and graduate students, as well as encourage diversity in the next generation of ocean scientists by providing summer internship opportunities for female middle school students from underrepresented communities (Project COOL).Most diagenetic indices for evaluating how "far along" organic matter is in its early diagenesis are based on amino acid compositions. Total hydrolyzable amino acid compositions indicate, in a coarse manner, organic matter degradation status, bioavailability, and source. Further development of amino acids as tools to assess geochemical processes requires including the wealth of information held within the peptide chains, information that is currently lost during hydrolysis. A powerful approach to sequencing detrital peptides is the application of tandem mass spectroscopy followed by de novo sequencing. In this project, researchers will use de novo sequencing to evaluate peptide and protein geochemistry during early diagenesis, and to develop a peptidoglycan-specific quantification method that allows utilization of the unique peptidoglycan peptide structures found in different bacteria. Specific actions to be taken under this project include (1) applying de novo sequencing to a set of well-characterized archive samples, (2) conducting a set of sorption experiments to evaluate the propensity for different detrital peptides to adhere to mineral surfaces and be protected from degradation, and (3) developing a quantitative method for measuring peptidoglycan peptides. By comparing detrital peptides to other known quantities of the sample set, relationships between organic matter diagenesis and peptide source, sequence, and preservation will be assessed.
生物、化学和物理过程改变了新近沉积的海洋沉积物。在这种通常被称为早期成岩作用的蚀变过程中,有机物的数量和组成发生了变化,这影响了海洋碳循环的关键组成部分。在这项研究中,研究人员将使用分析技术来评估早期成岩作用期间肽的地球化学,并开发评估细菌作用的方法。这项研究很重要,因为有机分子如肽影响海洋中碳和氮的降解速率和命运,因此对于理解这些关键营养物质的地球化学循环具有广泛的相关性。该项目还将为本科生和研究生提供研究机会,并通过为来自代表性不足社区的女中学生提供暑期实习机会(项目COOL),鼓励下一代海洋科学家的多样性。大多数用于评估有机物在其早期成岩作用中“有多沿着”的成岩指数都是以氨基酸组成为基础的。总的可水解氨基酸组成以粗略的方式指示有机物质降解状态、生物利用度和来源。进一步开发氨基酸作为评估地球化学过程的工具,需要包括肽链中所包含的丰富信息,这些信息目前在水解过程中丢失。一个强大的方法来测序碎片肽是应用串联质谱法,然后从头测序。在该项目中,研究人员将使用从头测序来评估早期成岩作用期间的肽和蛋白质地球化学,并开发一种肽聚糖特异性定量方法,该方法允许利用在不同细菌中发现的独特肽聚糖肽结构。在该项目下将采取的具体行动包括:(1)对一组已充分表征的档案样品进行从头测序;(2)进行一组吸附实验,以评估不同碎屑肽粘附在矿物表面并防止降解的倾向;以及(3)开发一种定量方法来测量肽聚糖肽。通过将碎屑肽与其他已知量的样品集进行比较,将评估有机质成岩作用与肽源、序列和保存之间的关系。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Richard Keil其他文献
Beiträge Entwickelungsgeschichte des Auges vom Schwein mit Besonderer Berücksichtigung des Verhaltens der Fötalen Augenspalte
- DOI:
10.1007/bf02214211 - 发表时间:
1906-10-01 - 期刊:
- 影响因子:2.900
- 作者:
Richard Keil - 通讯作者:
Richard Keil
Hoard of fjord carbon
峡湾碳的囤积
- DOI:
10.1038/ngeo2433 - 发表时间:
2015-05-04 - 期刊:
- 影响因子:16.100
- 作者:
Richard Keil - 通讯作者:
Richard Keil
Richard Keil的其他文献
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{{ truncateString('Richard Keil', 18)}}的其他基金
RECONSTRUCTING PAST ECOLOGIES USING PEPTIDOMICS
使用肽组学重建过去的生态学
- 批准号:
2122895 - 财政年份:2021
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
In Situ Experimentation to Determine the Impact of Sinking Particles on Dentrification and Anammox in Suboxic Waters
确定沉没颗粒对缺氧水中反硝化和厌氧氨氧化影响的原位实验
- 批准号:
1153935 - 财政年份:2012
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
Collaborative Research: Development of a low-cost PHOtosynthesis, Respiration, and Carbon balance Yielding System (PHORCYS)
合作研究:开发低成本光合作用、呼吸和碳平衡产生系统(PHORCYS)
- 批准号:
1155463 - 财政年份:2012
- 资助金额:
$ 39.92万 - 项目类别:
Continuing Grant
Project COOL: Where communities and academia come together
COOL 项目:社区和学术界汇聚的地方
- 批准号:
1202654 - 财政年份:2012
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
Collaborative Research: COSEE-Ocean Learning Communities - Connecting Communities around the World's Ocean
合作研究:COSEE-海洋学习社区 - 连接世界海洋社区
- 批准号:
1038836 - 财政年份:2010
- 资助金额:
$ 39.92万 - 项目类别:
Continuing Grant
OEDG: Sound Citizen Science Apprenticeship Program for Minority Youth (Track 1)
OEDG:针对少数族裔青年的健全公民科学学徒计划(轨道 1)
- 批准号:
0914483 - 财政年份:2009
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
Linking Protein Degradation to Peptide and Amino Acid Compositions in Sinking Particles and Marine Sediments
将蛋白质降解与下沉颗粒和海洋沉积物中的肽和氨基酸组成联系起来
- 批准号:
0926395 - 财政年份:2009
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
Impact of Suboxia on Sinking Particles: Non-Redfield Diagenesis and its Potential Role in C fluxes and N Cycling
Suboxia 对沉降颗粒的影响:非红场成岩作用及其在碳通量和氮循环中的潜在作用
- 批准号:
0726522 - 财政年份:2007
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
Collaborative Research: Ocean Learning Communities - Center for Ocean Science Education Excellence
合作研究:海洋学习社区 - 海洋科学教育卓越中心
- 批准号:
0528725 - 财政年份:2005
- 资助金额:
$ 39.92万 - 项目类别:
Continuing Grant
Organic-Mineral Interactions in Coastal Margin Sediments
沿海边缘沉积物中的有机矿物相互作用
- 批准号:
0454698 - 财政年份:2005
- 资助金额:
$ 39.92万 - 项目类别:
Standard Grant
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