Chemical and Structural Tools to Study Membrane Transport Proteins
研究膜转运蛋白的化学和结构工具
基本信息
- 批准号:1506420
- 负责人:
- 金额:$ 57.69万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-08-01 至 2019-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project is jointly funded by the Chemical Measurements and Imaging Program in the Division of Chemistry in the Directorate of Mathematical and Physical Sciences and the Cellular Dynamics and Function Cluster in the Division of Molecular and Cellular Biosciences in the Directorate for Biological Sciences.Proteins are biomolecules used by the cell to carry out a wide range of essential functions. The two major protein classes are often differentiated by their water solubility. While extensive methods exist to study the structure and dynamics of water-soluble proteins, water-insoluble proteins that are found within the cell membrane (membrane proteins) offer unique challenges related to their native location within lipid bilayers. In this project investigators will develop a toolbox of experiments to characterize the structure and motions of membrane proteins on the atomic level. The results of these experiments will enable for a more fundamental understanding of this class of proteins and their essential roles in biology. The broader impacts include the participation of high school, undergraduate, and graduate students, which will disseminate knowledge in research at the interface of chemistry, biology, and physics. The principal investigator of the project will also co-develop a new laboratory approach to teach historically important experiments in biochemistry with the goal of increasing knowledge retention and achievement. Although the importance of membrane proteins have been well documented in signal transduction, molecular transport, and regulation of intracellular ion concentrations, less than 2% of known protein structures consist of these hydrophobic biomolecules. This research project will develop novel solid-state NMR techniques aimed at overcoming the major hurdles plaguing the characterization of membrane protein structure and dynamics. Specifically, the intellectual merits of this research include: (a) spectroscopic innovation aimed at improving the sensitivity and resolution of membrane protein spectra under native-like conditions in lipid bilayers, (b) new approaches to characterize the orientation of membrane proteins relative to the lipid bilayer, and (c) methods to study the mechanism of ion-coupled transport. The developments of this research will improve the feasibility of characterizing large, polytopic helical membrane proteins in lipid bilayers. The broader impacts of these research activities will be to (a) increase and disseminate knowledge in the interdisciplinary field of biophysical chemistry, (b) give research opportunities to and mentorship of high school and undergraduate students, and (c) emphasize the importance of clear and concise communication to promote science and technology. The PI's activities include the participation of undergraduate and high school students (e.g., ACS Project SEED, GSTEM, and School of the Future) from underrepresented groups in science and technology, including minorities, women, and those with disabilities. The involvement of young science-minded students in research is aimed at increasing retention and improving graduate rates in STEM fields. From an educational perspective, the PI will also take an active role in co-developing a Historical Experiments in Biochemistry lab course at Spelman College with Prof. Kimberly Jackson that will emphasize important experiments in the history of biochemistry. The outcomes of the project will be disseminated to the Faculty Resource Network at New York University with the goal of bolstering achievement in undergraduate teaching and biochemistry curricula across the 50+ member institutions.
该项目由数学和物理科学理事会化学部的化学测量和成像计划以及生物科学理事会分子和细胞生物科学部的细胞动力学和功能集群共同资助。蛋白质是细胞用于执行广泛基本功能的生物分子。这两种主要的蛋白质类别通常通过它们的水溶性来区分。虽然存在广泛的方法来研究水溶性蛋白质的结构和动力学,但在细胞膜内发现的水不溶性蛋白质(膜蛋白)提供了与其在脂质双层内的天然位置相关的独特挑战。在这个项目中,研究人员将开发一个实验工具箱,以在原子水平上表征膜蛋白的结构和运动。 这些实验的结果将使我们能够更基本地了解这类蛋白质及其在生物学中的重要作用。更广泛的影响包括高中生,本科生和研究生的参与,这将传播化学,生物学和物理学接口的研究知识。该项目的主要研究者还将共同开发一种新的实验室方法,以教授具有历史意义的生物化学实验,目的是提高知识的保留和成就。尽管膜蛋白在信号转导、分子转运和细胞内离子浓度调节中的重要性已被充分证明,但只有不到2%的已知蛋白质结构由这些疏水生物分子组成。本研究计划将发展新型固态核磁共振技术,以克服膜蛋白结构和动力学表征的主要障碍。具体而言,这项研究的智力优点包括:(a)光谱创新,旨在提高膜蛋白光谱的灵敏度和分辨率的天然条件下,在脂质双层,(B)新的方法来表征膜蛋白相对于脂质双层的取向,和(c)的方法来研究离子耦合传输的机制。这项研究的进展将提高表征脂双层中大型多位螺旋膜蛋白的可行性。这些研究活动的广泛影响将是:(a)增加和传播生物物理化学跨学科领域的知识;(B)为高中生和本科生提供研究机会和指导;(c)强调简明扼要的交流对促进科学和技术的重要性。PI的活动包括本科生和高中生的参与(例如,ACS项目种子,GSTEM和未来的学校)从科学和技术,包括少数民族,妇女和残疾人代表性不足的群体。具有科学头脑的年轻学生参与研究的目的是提高STEM领域的保留率和毕业率。从教育的角度来看,PI还将积极参与Spelman学院与Kimberly杰克逊教授共同开发生物化学实验室历史实验课程,该课程将强调生物化学历史上的重要实验。该项目的成果将传播到纽约大学的教师资源网络,其目标是在50多个成员机构中加强本科教学和生物化学课程的成就。
项目成果
期刊论文数量(0)
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专利数量(0)
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Nathaniel Traaseth其他文献
Topology of Phospholamban when Bound to Ca2±ATPase by Solid-State NMR
- DOI:
10.1016/j.bpj.2008.12.2210 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Nathaniel Traaseth;Raffaello Verardi;Lei Shi;Gianluigi Veglia - 通讯作者:
Gianluigi Veglia
Deciphering Transport Mechanisms of Bacterial Efflux Pumps using NMR Spectroscopy
- DOI:
10.1016/j.bpj.2017.11.1157 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Maureen Leninger;Ampon Sae Her;Casey Mueller;James Banigan;Nathaniel Traaseth - 通讯作者:
Nathaniel Traaseth
Nathaniel Traaseth的其他文献
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{{ truncateString('Nathaniel Traaseth', 18)}}的其他基金
Tools for Probing Conformational Dynamics of Membrane Proteins
探测膜蛋白构象动力学的工具
- 批准号:
1902449 - 财政年份:2019
- 资助金额:
$ 57.69万 - 项目类别:
Standard Grant
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