Peptide Derivatized Poly(ester urea)s for Regenerative Medicine

用于再生医学的肽衍生聚（酯脲）

• 批准号: 1507420
• 负责人: Matthew Becker
• 金额: $ 39万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1507420&HistoricalAwards=false
• 关键词: Peptide; Derivatized; Poly; ester; urea

Nontechnical: This award by the Biomaterials program in the Division of Materials Research to the University of Akron aims at discovering systematic method for controllably tethering bioactive peptides to novel polymer scaffolds for use in regenerative medicine applications. This award is also co-funded by the Biomedical Engineering program in CBET/ENG. If successful, this work will optimize peptide concentrations that enhance cell proliferation and accelerate stem cell differentiation. This knowledge would help guide the rational design of future peptide-functionalized polymer surfaces, scaffolds and hydrogels used by the wider tissue engineering community. The biological measurements will drive the design of advanced bioactive and biomimetic materials for use in many applications where synthetic materials contact biological systems. The research team is involved in the educational training components of the project. The effort will provide advanced, multidisciplinary training to undergraduate and graduate students, orthopedic surgery residents, and high school students in areas of molecular and cell biology, chemistry and polymer science. The outcomes from the research are being directly incorporated into graduate level and undergraduate courses being taught by Professor Becker. Undergraduate students from the University of Akron, and summer interns enrolled in the research experiences for undergraduates (REU) program will participate extensively. The research team's outreach activities include mentoring and provide educational and research experience for students from local high schools.Technical: This award by the Biomaterials program in the Division of Materials Research and the Biomedical Engineering program in CBET/ENG to the University of Akron aims at discovering a systematic method for controllably tethering multiple bioactive peptides to novel poly(ester urea) scaffolds post-3D printing for use in regenerative medicine applications. The biomaterials community clearly understands that the molecular presentation and spatial distribution of peptides and growth factors can dramatically influence many important aspects of cell behavior. This award aims to translate previously discovered GRGDS and BMP-2 peptide concentrations to translationally-relevant degradable poly(ester urea) constructs in two-dimensional thin films and three-dimensional printed scaffolds. The project will impact several fields of biomaterials including (1) developing new methods for functionalization of degradable polymer scaffolds with bioactive peptides post 3D printing and (2) new methods for characterizing surface concentrations of these peptides and (3) identification of synergistic peptide concentrations that influence hMSC cell proliferation, lineage commitment, and differentiation. The project offers multiple opportunities for the educational training of students (both undergraduate and graduate) and medical residents in advanced polymer science, state of the art chemical functionalization and translationally-relevant strategies for advanced manufacturing of 3D scaffolds. These skills will be critical to public and private entities aiming to move 3D printed scaffolds into mainstream device and regenerative medicine applications.

非技术性：该奖项由阿克伦大学材料研究部生物材料计划颁发，旨在发现可控制地将生物活性多肽与用于再生医学应用的新型聚合物支架捆绑在一起的系统方法。该奖项也由CBET/ENG的生物医学工程项目共同资助。如果成功，这项工作将优化促进细胞增殖和加速干细胞分化的多肽浓度。这些知识将有助于指导未来更广泛的组织工程界使用的多肽功能化聚合物表面、支架和水凝胶的合理设计。生物测量将推动先进的生物活性和仿生材料的设计，用于合成材料接触生物系统的许多应用。研究小组参与了该项目的教育培训部分。这项努力将为本科生和研究生、整形外科住院医生和高中生提供分子和细胞生物学、化学和聚合物科学领域的高级、多学科培训。这项研究的结果将被直接纳入贝克尔教授教授的研究生和本科课程。阿克伦大学的本科生和暑期实习生将广泛参与本科生研究体验(REU)项目。研究团队的推广活动包括指导当地高中生，并为他们提供教育和研究经验。技术：该奖项由材料研究部的生物材料计划和CBET/ENG的生物医学工程计划授予阿克伦大学，旨在发现一种系统的方法，在3D打印后将多个生物活性多肽可控地捆绑到新型聚酯尿素支架上，用于再生医学应用。生物材料界清楚地认识到，多肽和生长因子的分子呈现和空间分布可以极大地影响细胞行为的许多重要方面。该奖项旨在将之前发现的GRGDS和BMP-2多肽浓度转化为二维薄膜和三维打印支架中与翻译相关的可降解聚(酯)尿素结构。该项目将对生物材料的几个领域产生影响，包括(1)开发3D打印后带有生物活性多肽的可降解聚合物支架的功能化新方法，(2)表征这些多肽表面浓度的新方法，以及(3)鉴定影响hMSC细胞增殖、谱系承诺和分化的协同多肽浓度。该项目为学生(本科生和研究生)和住院医生提供了多种教育培训机会，内容包括先进的聚合物科学、最先进的化学功能化以及先进的3D支架制造的翻译相关策略。这些技能将对旨在将3D打印支架应用于主流设备和再生医学应用的公共和私人实体至关重要。