UNS: A Novel Algal-Bacterial Shortcut Nitrogen Removal Process for Wastewater Treatment

UNS：一种用于废水处理的新型藻菌捷径脱氮工艺

• 批准号: 1511439
• 负责人: Sarina Ergas
• 金额: $ 33万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1511439&HistoricalAwards=false
• 关键词: UNS; Novel; Algal; Bacterial; Shortcut

1511439ErgasManagement of the nitrogen cycle was identified by the National Academy of Engineering (NAE) as one of the grand challenges of the 21st century. High ammonia strength wastewaters, such as anaerobic digestion effluents, are difficult and costly to treat in conventional biological nitrogen removal systems due to their toxicity and high oxygen requirements. The PIs overall goal is to investigate nitrogen metabolism in algal-bacterial shortcut nitrogen removal and identify the optimum conditions needed to sustain this novel process. The proposed research is significant because it has the potential to reduce the costs, energy requirements and greenhouse gas emissions associated with nitrogen removal from wastewaters. The guiding hypothesis is that the close association of algae and bacteria and light/dark cycles results in conditions within biological particles that favor shortcut nitrogen removal.Through interdisciplinary and international collaboration, the PIs propose to: 1) investigate the effects of varying operating conditions and substrates on system kinetics and performance, 2) investigate the microbial consortium, which includes algae, ammonia oxidizing bacteria, nitrite oxidizing bacteria, and denitrifying bacteria using molecular tools, 3) optimize the system design through coupled process-optimization modeling, and, 4) develop global competency in US students though an international collaboration. The interrelationships between nitrogen loading, light penetration and oxygen production will be studied using bench-scale SBPRs operated at varying illumination, temperature and loading rates. The presence and activities of algae nitrogen metabolizing microorganisms will be tracked via measurements of key chemical constituents and by using molecular biological tools to target functional genes encoding steps in the N cycle relevant to nitrification/ denitrification. Additional experiments will investigate the use of volatile fatty acids harvested from livestock waste fermentation as an electron donor for denitrification and coupling of partial algal-bacterial ammonia oxidation with anammox technology to improve system economics. A framework that couples an algal-bacterial shortcut nitrogen process models and an optimization models will be used to minimize system footprint with target total nitrogen removal as a constraint. The optimization model will allow the PIs to find the optimal operating conditions to minimize reactor footprint for different geographic regions. The PIs have assembled a team of researchers in the US and Netherlands with expertise in biological nitrogen removal and algae production, autotrophic bacterial physiology, and wastewater process and optimization modeling. Graduate and undergraduate students, secondary science teachers and HS students will receive training in interdisciplinary, globalized science and engineering research in developed and developing world contexts. We will build on student exchanges between University of South Florida and UNESCO-IHE in Delft, the Netherlands and University of South Florida's Peace Corps Masters International Program to gain additional resources (students, facilities, and research expertise), build developing world sustainable water treatment capacity and continue co-development of the shortcut algal-bacterial nitrogen removal process.

美国国家工程学院（NAE）将1511439的氮周期管理确定为21世纪的巨大挑战之一。高氨强度废水（例如厌氧消化废水）由于其毒性和高氧需求而难以在常规的生物氮去除系统中治疗很难治疗。 PIS的总体目标是研究藻类 - 细菌快捷方式氮的去除氮中的氮代谢，并确定维持这种新过程所需的最佳条件。拟议的研究很重要，因为它有可能降低与废水中去除氮相关的成本，能源需求和温室气体排放。 The guiding hypothesis is that the close association of algae and bacteria and light/dark cycles results in conditions within biological particles that favor shortcut nitrogen removal.Through interdisciplinary and international collaboration, the PIs propose to: 1) investigate the effects of varying operating conditions and substrates on system kinetics and performance, 2) investigate the microbial consortium, which includes algae, ammonia oxidizing bacteria,亚硝酸盐氧化细菌，并使用分子工具来硝化细菌，3）通过耦合过程 - 优化建模来优化系统设计，以及4）通过国际协作发展美国学生的全球能力。将使用以不同照明，温度和加载速率操作的台式SBPRS研究氮气负荷，光穿透和氧气产生之间的相互关系。藻类氮代谢微生物的存在和活性将通过测量关键化学成分的测量以及使用分子生物学工具来靶向与硝化/非硝化相关的N周期中编码步骤的功能基因。其他实验将调查从牲畜废物发酵中收获的挥发性脂肪酸作为一种电子供体，用于反硝化和部分藻类细菌氨氧化与Anammox技术的偶联，以改善系统经济学。将藻类 - 细菌快捷方式氮工艺模型和优化模型耦合的框架将用于最大程度地减少系统足迹，而目标总氮去除作为约束。优化模型将允许PI找到最佳的工作条件，以最大程度地减少不同地理区域的反应堆足迹。 PI已在美国和荷兰组成了一组研究人员，在生物氮去除和藻类生产，自养细菌生理学以及废水过程和优化建模方面具有专业知识。研究生和本科生，中学教师和HS学生将接受发达和发展世界环境中的跨学科，全球化科学和工程研究的培训。我们将建立基于南佛罗里达大学和代尔夫特大学，荷兰和南佛罗里达大学和平队国际计划之间的学生交流，以获得额外的资源（学生，设施和研究专业知识），增强发展中国家的可持续水处理能力，并继续共同开发快捷的Algal algal-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial-Bacterial nitrogen Remogen reepropal。