UNS: COLLABORATIVE RESEARCH: Nanoengineering biomimetic nanobrushes for pathogen sensing

合作项目1512659戈麦斯，卡门1511953麦克拉莫尔，埃里克S。这项研究将开发一种新的传感器设备，旨在检测食源性病原体。 研究人员将刺激反应材料与专门开发的生物分子结合，用于检测单核细胞增生李斯特菌（食品中存在的病原体）。 这项工作特别重要，因为我们没有对这种特定病原体具有高亲和力的抗体（识别病原体的分子）。本研究的目的是开发结合联合收割机刺激响应性聚合物纳米刷和适体的生物传感器。这种方法有望显著改善病原体检测领域。生物传感器设备将在广泛的标准上进行评价，例如灵敏度、选择性和响应时间，并进行适当的控制。 该建议的智力价值在于将刺激响应性聚合物的致动与通过固定在聚合物表面上的适体对细菌的选择性捕获相结合，以增加对靶病原体的选择性捕获和保留。在这项工作中提出的仿生方法的灵感来自于选择性招聘的共生细菌的纤毛虫驱动在短尾鱿鱼轻器官，这是能够捕获一个特定的目标物种从一个复杂的微生物背景和广泛的颗粒大小的解决方案。当与纳米材料修饰的电极组合时，预期提供两个明显的优点;即，由于较低的非特异性结合而增强对靶细菌的捕获，以及增强的检测限。 更广泛的影响：方便、快速、灵敏的食源性病原体生物传感器在食品安全和医学诊断中具有重要意义。 这项拟议的研究也是向两个合作机构代表性不足的人口传播工程研究的独特机会，并将侧重于食品安全和纳米生物传感技术。这些活动将包括在得克萨斯州A& M大学：（i）在“拓展你的视野”一日营中展示检测食源性病原体的实际应用（每年有70名六年级女生参加）;（ii）通过“妇女探索工程学”夏令营指导和接待年轻妇女;（iii）通过“丰富工程学经验”方案接待一名高中教师。在佛罗里达大学，活动将包括（i）通过大学前教育和培训中心招募中学科学教育工作者参与研究项目;（ii）使用动画软件开发在线学习模块，教授生物传感器概念;以及（iii）通过4 H生物工程夏令营协调和接待K-12学生

Collaborative Project 1512659 Gomes, Carmen 1511953 McLamore, Eric S. This research will develop a novel sensor device designed to detect foodborne pathogens. The researchers will stimuli-response material in combination with a specially developed biomolecule for detecting Listeria monocytogenes (pathogen present in foods). The proposed work is particularly important because we do not have antibodies (a molecule that recognizes the pathogen) that have high affinity for this particular pathogen. The objective of this research is to develop biosensors which combine stimuli-responsive polymer nanobrushes and aptamers. Such an approach is expected to significantly improve the field of pathogen detection. The biosensor device will be evaluated on a broad range of criteria such as sensitivity, selectivity, and response time with appropriate controls. The intellectual merit of this proposal lies in combining the actuation of stimuli-responsive polymers with selective capture of bacteria by aptamers immobilized on the polymer surface for increasing selective capture and retention of target pathogens. The biomimetic approach proposed in this work is inspired by selective recruitment of symbiotic bacteria by ciliate actuation in the bobtail squid light organ, which is capable of capturing a specific target species from a solution with a complex microbial background and a wide range of particle sizes. When combined with nanomaterial-modified electrodes, it is anticipated to provide two distinct advantages; namely, enhanced capture of target bacteria due to lower non-specific binding, and enhanced limit of detection. Broader Impacts : A convenient, rapid, and sensitive biosensor for foodborne pathogens can have significant impact in food safety and medical diagnostics. This proposed research also serves as a unique opportunity for dissemination of engineering research to underrepresented population at the two collaborating institutions and will focus on food safety and nano-biosensing technologies. These activities will include at Texas A&M University: (i) demonstrating practical applications on detecting foodborne pathogens in the Expanding Your Horizon one-day camp ( reaches 70 sixth-grade girls per year), (ii) mentoring and hosting young women through the Women Explore Engineering summer camp; and (iii) hosting a high school teacher through Enrichment Experiences in Engineering program. At University of Florida activities will include (i) recruiting secondary science educator to participate in the research project through the Center for Precollegiate Education and Training (ii) developing online learning modules using animation software to teach biosensor concepts; and (iii) coordinating and hosting K-12 students through 4H Bioengineering summer camp