UNS: COLLABORATIVE RESEARCH: Nanoengineering biomimetic nanobrushes for pathogen sensing

UNS：合作研究：用于病原体传感的纳米工程仿生纳米刷

• 批准号: 1512659
• 负责人: Zivko Nikolov
• 金额: $ 18万
• 依托单位: Texas A&M Engineering Experiment Station
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1512659&HistoricalAwards=false
• 关键词: UNS; COLLABORATIVE; RESEARCH; Nanoengineering; biomimetic

Collaborative Project 1512659 Gomes, Carmen 1511953 McLamore, Eric S. This research will develop a novel sensor device designed to detect foodborne pathogens. The researchers will stimuli-response material in combination with a specially developed biomolecule for detecting Listeria monocytogenes (pathogen present in foods). The proposed work is particularly important because we do not have antibodies (a molecule that recognizes the pathogen) that have high affinity for this particular pathogen. The objective of this research is to develop biosensors which combine stimuli-responsive polymer nanobrushes and aptamers. Such an approach is expected to significantly improve the field of pathogen detection. The biosensor device will be evaluated on a broad range of criteria such as sensitivity, selectivity, and response time with appropriate controls. The intellectual merit of this proposal lies in combining the actuation of stimuli-responsive polymers with selective capture of bacteria by aptamers immobilized on the polymer surface for increasing selective capture and retention of target pathogens. The biomimetic approach proposed in this work is inspired by selective recruitment of symbiotic bacteria by ciliate actuation in the bobtail squid light organ, which is capable of capturing a specific target species from a solution with a complex microbial background and a wide range of particle sizes. When combined with nanomaterial-modified electrodes, it is anticipated to provide two distinct advantages; namely, enhanced capture of target bacteria due to lower non-specific binding, and enhanced limit of detection. Broader Impacts : A convenient, rapid, and sensitive biosensor for foodborne pathogens can have significant impact in food safety and medical diagnostics. This proposed research also serves as a unique opportunity for dissemination of engineering research to underrepresented population at the two collaborating institutions and will focus on food safety and nano-biosensing technologies. These activities will include at Texas A&M University: (i) demonstrating practical applications on detecting foodborne pathogens in the Expanding Your Horizon one-day camp ( reaches 70 sixth-grade girls per year), (ii) mentoring and hosting young women through the Women Explore Engineering summer camp; and (iii) hosting a high school teacher through Enrichment Experiences in Engineering program. At University of Florida activities will include (i) recruiting secondary science educator to participate in the research project through the Center for Precollegiate Education and Training (ii) developing online learning modules using animation software to teach biosensor concepts; and (iii) coordinating and hosting K-12 students through 4H Bioengineering summer camp

合作项目1512659戈麦斯，卡门1511953麦克拉莫尔，埃里克S。这项研究将开发一种新的传感器设备，旨在检测食源性病原体。研究人员将刺激反应材料与一种专门开发的生物分子相结合，用于检测单核细胞增多性李斯特菌(食品中存在的病原体)。这项拟议的工作特别重要，因为我们没有对这种特定病原体具有高亲和力的抗体(识别病原体的分子)。这项研究的目的是开发结合刺激响应性聚合物纳米脉冲和适体的生物传感器。这种方法有望显著改善病原体检测领域。生物传感器装置将根据一系列标准进行评估，如灵敏度、选择性和响应时间，并进行适当的控制。这一建议的智力优点在于将刺激响应聚合物的激活与固定在聚合物表面的适体选择性捕获细菌相结合，以增加对目标病原体的选择性捕获和保留。这项工作中提出的仿生方法的灵感来自于通过纤毛虫驱动在短尾鱿鱼发光器官中选择性地招募共生细菌，该方法能够从具有复杂微生物背景和广泛颗粒大小的溶液中捕获特定的目标物种。当与纳米材料修饰的电极结合时，它有望提供两个明显的优点：即由于较低的非特异性结合而增强了对目标细菌的捕获，以及提高了检测限。更广泛的影响：一种方便、快速和灵敏的食源性病原体生物传感器可以在食品安全和医疗诊断方面产生重大影响。这项拟议的研究也是向两个合作机构中代表性不足的人群传播工程研究的独特机会，并将侧重于食品安全和纳米生物传感技术。这些活动将包括在德克萨斯农工大学：(I)在扩大你的地平线一日营(每年有70名六年级女孩参加)中展示在检测食源性病原体方面的实际应用；(Ii)通过女性探索工程夏令营指导和接待年轻女性；以及(Iii)通过丰富工程经验计划接待一名高中教师。在佛罗里达大学的活动将包括：(I)通过大学前教育和培训中心招募中等科学教育人员参与研究项目；(Ii)使用动画软件开发在线学习模块，教授生物传感器概念；以及(Iii)通过4H生物工程夏令营协调和接待K-12学生