Mechanistic Analysis of How a Plant DNA Methylase Acts on Chromatin

点击翻译按钮获取中文摘要

This project is funded jointly by the Genetic Mechanisms Cluster in the Division of Molecular and Cellular Biosciences and the Plant Genome Research Program in the Division of Integrative Organismal Systems in the Directorate for Biological Sciences.Cell identity is determined and maintained by selective expression of a group of genes. This project will help uncover new molecular mechanisms for how large regions of the inherited genome are turned off or left unread, forming different types of cells in organisms like plants. To do this, structures called heterochromatin must be created and manipulated. Inside heterochromatin are structures called nucleosomes, which are packages of DNA wrapped proteins that help organize the DNA strands. The mechanism of an important protein in maize (corn), which may reduce the availability of DNA to protein interactions responsible for reading DNA will be investigated. This program will attempt to find answers to two questions: (1) how this protein chemically changes the DNA located in nucleosomes, and (2) how this protein alters nucleosome packing to create structures that can turn off or silence certain genes. Multi-disciplinary training will be given to the participating students in the form of combining a variety of biophysical approaches, as well as genetic approaches. Active participation of under-represented minorities through a partnership with the principle investigator's laboratory and the San Francisco State University's Masters Program, and leading participation by women graduate students in this program will encourage these students to apply bio-regulatory concepts to real problems in a team setting. The results from this research will also build towards a better understanding of how heterochromatin based silencing mechanisms interfere in the formation of crops with pest-resistance and enhanced growth and nutritional content.In plants and mammals heterochromatin is defined by the presence of two post-translational modifications, histone H3 lysine 9 methylation (H3K9me) and DNA cytosine methylation as well as specific protein factors that recognize these marks. In both contexts two properties of heterochromatin are considered to be essential for its function: (i) the ability to spread to adjacent genomic regions from the sites of initiation, which enables action across large stretches of the genome and (ii) the ability to condense the underlying chromatin, which is thought to inhibit DNA access and make the chromatin refractory to transcription. However the biochemical basis for how DNA and histone methylation enable repressive chromatin structures is poorly understood. The major cytosine DNA methyltransferase, CMT3 from Arabidopsis thaliana and its maize homolog ZMET2, provide an opportunity to tackle this fundamental question. CMT3 and ZMET2 have two accessory domains that recognize the H3K9 methyl mark and this recognition is essential for DNA methylation in vivo. This project will test the following hypotheses: (i) ZMET2 bridges H3K9me nucleosomes and compacts the underlying chromatin and (ii) ZMET2 activity is controlled by defined chromatin architectures. The project combines mechanistic enzymology with methods such as multi-angle light scattering, FRET and cutting edge electron cryo-microscopy. The models derived from the work on ZMET2 will be tested in vivo in the context of the related CMT3 enzyme in Arabidopsis thaliana. The mechanistic dissection here is expected to: (i) elucidate the biophysical basis for how DNA methyltransferases collaborate with histone methylation to generate heterochromatin and (ii) uncover new regulatable steps in heterochromatin formation.