I-Corps: Silk Stabilization Technologies

目前普遍存在的存储和运输问题限制了诊断测试的准确性和用于远程使用的坚固的测试设备的分发。这些问题源于敏感定量所需的分子和试剂固有的温度稳定性，包括抗体、酶、蛋白质标准和测试样本本身(即全血、唾液、尿液等)。这个i-Corps团队提出了一种基于丝素蛋白作为稳定剂的技术平台，可以用来保护这些分子在常温下不被降解，这反过来可以扩大现场部署的诊断设备的使用案例，降低与冷藏相关的成本，延长宝贵的内部标准品或传感器上固定的化学物质的保质期，并有可能使流体生物样本的检索更加可行。丝素技术平台为诊断领域的商业发展提供了许多潜在的途径。该小组开展的初步工作侧重于调整蚕丝的提纯和格式化，以改进其稳定功能(即热保护/改进的恢复)，以满足现场环境的需要。该团队最近还展示了丝素基质在多种测试标记上提供的热稳定性增强，并展示了与许多不同的测试技术的兼容性，如免疫分析、定量实时聚合酶链式反应和各种高通量方法。该团队建议将这些核心概念简化为对热稳定性较差的现有产品进行改造，并开发由该技术实现的新产品，包括护理点设备和含有不稳定试剂的样品瓶。I-Corps基金将支持开发一种原型设备，该设备包含干燥的丝绸基质，可用于在符合人体工程学的廉价消耗品上捕获和干燥毛细血管血液。

There are pervasive storage and transport problems that currently limit the accuracy of diagnostic tests and distribution of robust testing devices for remote usage. These problems stem from the inherent temperature stabilities of the molecules and reagents required for sensitive quantitation including antibodies, enzymes, protein standards, and the test specimens themselves (i.e. whole blood, saliva, urine, etc.). This I-Corps team proposes a technology platform based on using silk fibroin as a stabilizer that can be used to protect these molecules from degradation at ambient temperatures, which in turn can expand the use cases of diagnostic devices deployed in the field, will reduce the costs associated with cold storage, improve the shelf life of precious internal standards or immobilized chemistries on sensors, and potentially make the retrieval of fluid bio-specimens more feasible.The silk technology platform offers many potential avenues for commercial development within the field of diagnostics. The initial work conducted by this team has focused on adapting silk purification and formatting to improve its stabilizing features (i.e. thermal protection / improved recovery) for needs in field settings. The team has also recently demonstrated thermal stability enhancements provided by the silk matrix across a wide number of test markers and demonstrated compatibility with numerous distinct testing techniques such as immunoassays, quantitative real-time PCR, and various high-throughput approaches. The team proposes the reduction of these core concepts to the retrofitting of existing products with poor thermal stability and the development of new products enabled by the technology, including point-of-care devices and sample vials containing labile reagents. I-Corps funds will support the development of a prototype device that incorporates a dried silk matrix and can be used to capture and dry capillary blood on an ergonomic and inexpensive consumable.